2021
A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin.
Brown S, Ren Y, Suter D, Mattoo S. A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin. Bio-protocol 2021, 11: e4158. PMID: 34692908, PMCID: PMC8481015, DOI: 10.21769/bioprotoc.4158.Peer-Reviewed Original ResearchPhosphorylation of cortactinActin cytoskeletal dynamicsNon-phosphorylated proteinActin-binding proteinsRecombinant protein expressionCell-free systemTumor cell metastasisNeuronal growth conesBacterial kinasesLamellipodia protrusionBag cell neuronsPhosphorylation assaysNon-neuronal cellsFilopodia formationSrc kinaseInduced proteinsCortactinTyrosine kinaseEfficient expressionKinaseCell migrationPhosphorylationBiochemical studiesCell metastasisGrowth cones
2019
A single tyrosine phosphorylation site in cortactin is important for filopodia formation in neuronal growth cones
Ren Y, He Y, Brown S, Zbornik E, Mlodzianoski M, Ma D, Huang F, Mattoo S, Suter D. A single tyrosine phosphorylation site in cortactin is important for filopodia formation in neuronal growth cones. Molecular Biology Of The Cell 2019, 30: 1817-1833. PMID: 31116646, PMCID: PMC6727743, DOI: 10.1091/mbc.e18-04-0202.Peer-Reviewed Original ResearchConceptsTyrosine phosphorylation sitesPhosphorylation sitesFilopodia formationNeuronal growth conesSingle tyrosine phosphorylation sitePhosphorylated cortactinGrowth conesPhospho-mimetic mutantVitro kinase assaysFilopodia lengthFunction of cortactinTyrosine-phosphorylated cortactinFormation of filopodiaSrc tyrosine kinaseCortactin mutantMultiple actinPhosphorylation substratesActin organizationCellular processesLength of filopodiaKinase assaysCortactinTyrosine kinaseNonneuronal cellsFilopodia