2011
High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells
Lee MY, Bozkulak E, Schliffke S, Amos PJ, Ren Y, Ge X, Ehrlich BE, Qyang Y. High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells. Biochemical And Biophysical Research Communications 2011, 416: 51-57. PMID: 22079290, PMCID: PMC3237870, DOI: 10.1016/j.bbrc.2011.10.140.Peer-Reviewed Original ResearchConceptsMurine embryonic stem cellsEmbryonic stem cellsBone morphogenetic proteinEB culturesCardiac differentiationCardiomyocyte productionEmbryoid body culturesStem cellsCardiac-specific genesEarly cardiac developmentCardiogenic growth factorsNK2 transcription factorCardiac regenerative medicineMESC linesTranscription factorsCardiac developmentMorphogenetic proteinsEmbryoid bodiesDifferentiation systemDifferentiation periodFunctional cardiomyocytesHigh-density culturesMyosin light chain 2vRegenerative medicineCell-based therapies
1999
Cell-Type-Dependent Activity of the Ubiquitous Transcription Factor USF in Cellular Proliferation and Transcriptional Activation
Qyang Y, Luo X, Lu T, Ismail P, Krylov D, Vinson C, Sawadogo M. Cell-Type-Dependent Activity of the Ubiquitous Transcription Factor USF in Cellular Proliferation and Transcriptional Activation. Molecular And Cellular Biology 1999, 19: 1508-1517. PMID: 9891084, PMCID: PMC116079, DOI: 10.1128/mcb.19.2.1508.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCell DivisionCell LineDNADNA-Binding ProteinsGene ExpressionGenes, ReporterHeLa CellsHumansImmediate-Early ProteinsModels, BiologicalMolecular Sequence DataRecombinant Fusion ProteinsSubcellular FractionsTrans-ActivatorsTranscription FactorsTranscriptional ActivationTransfectionUpstream Stimulatory FactorsViral Envelope ProteinsConceptsUSF-specific regionDNA-binding activityUSF proteinsSaos-2 cellsTranscriptional activationAutonomous transcriptional activation domainsTranscriptional activityTranscriptional activation domainTranscription factor USFActivity of USFHelix transcription factorCellular proliferationCell linesActivation domainUSF activityUSF functionSaos-2 osteosarcoma cell lineSubcellular localizationTranscription factorsInitiator elementReporter geneMutational analysisTransient cotransfectionFusion proteinOsteosarcoma cell lines