2012
A Designed Zinc-finger Transcriptional Repressor of Phospholamban Improves Function of the Failing Heart
Zhang HS, Liu D, Huang Y, Schmidt S, Hickey R, Guschin D, Su H, Jovin IS, Kunis M, Hinkley S, Liang Y, Hinh L, Spratt SK, Case CC, Rebar EJ, Ehrlich BE, Ehrlich B, Gregory P, Giordano F. A Designed Zinc-finger Transcriptional Repressor of Phospholamban Improves Function of the Failing Heart. Molecular Therapy 2012, 20: 1508-1515. PMID: 22828502, PMCID: PMC3412484, DOI: 10.1038/mt.2012.80.Peer-Reviewed Original ResearchConceptsHeart failureZinc finger protein transcription factorsSingle gene regulationZinc finger transcriptional repressorDiverse DNA sequencesProtein transcription factorsDisease-related genesDisease-related proteinsGene repressionZFP TFsTranscriptional repressorTranscription factorsDNA sequencesPotent repressionPLN expressionHuman diseasesRepressorContractile functionDrug targetsFailing HeartTherapeutic inhibitionAnimal modelsReuptake kineticsRepressionTherapeutic interventions
2006
p204 Protein Overcomes the Inhibition of the Differentiation of P19 Murine Embryonal Carcinoma Cells to Beating Cardiac Myocytes by Id Proteins*
Ding B, Liu CJ, Huang Y, Yu J, Kong W, Lengyel P. p204 Protein Overcomes the Inhibition of the Differentiation of P19 Murine Embryonal Carcinoma Cells to Beating Cardiac Myocytes by Id Proteins*. Journal Of Biological Chemistry 2006, 281: 14893-14906. PMID: 16556596, DOI: 10.1074/jbc.m511748200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell Line, TumorFeedback, PhysiologicalGATA4 Transcription FactorGene Expression RegulationHomeobox Protein Nkx-2.5Homeodomain ProteinsInhibitor of Differentiation Protein 1Inhibitor of Differentiation Protein 2Inhibitor of Differentiation ProteinsMiceMyocytes, CardiacNuclear ProteinsPhosphoproteinsTranscription FactorsTranscriptional ActivationConceptsD proteinsP204 proteinP19 cellsP19 murine embryonal carcinoma cellsEmbryonal carcinoma stem cellsNuclear export signalMurine embryonal carcinoma cellsP19 embryonal carcinoma stem cellsCardiac transcription factor GATA4Embryonal carcinoma cellsTranscription factor GATA4Carcinoma stem cellsExport signalSynergistic transactivationTranscription factorsNKX2.5 proteinPositive feedback loopCardiac myocytesDifferentiation proteinGATA4ProteinStem cellsDifferentiationP204Carcinoma cellsp204 Is Required for the Differentiation of P19 Murine Embryonal Carcinoma Cells to Beating Cardiac Myocytes ITS EXPRESSION IS ACTIVATED BY THE CARDIAC GATA4, NKX2.5, AND TBX5 PROTEINS*
Ding B, Liu CJ, Huang Y, Hickey RP, Yu J, Kong W, Lengyel P. p204 Is Required for the Differentiation of P19 Murine Embryonal Carcinoma Cells to Beating Cardiac Myocytes ITS EXPRESSION IS ACTIVATED BY THE CARDIAC GATA4, NKX2.5, AND TBX5 PROTEINS*. Journal Of Biological Chemistry 2006, 281: 14882-14892. PMID: 16556595, DOI: 10.1074/jbc.m511747200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCell Line, TumorChromatinGATA4 Transcription FactorGene Expression Regulation, DevelopmentalHomeobox Protein Nkx-2.5Homeodomain ProteinsMiceMolecular Sequence DataMuscle, SkeletalMyocytes, CardiacNuclear ProteinsPhosphoproteinsT-Box Domain ProteinsTranscription FactorsConceptsNuclear export signalExport signalTranscription factorsP19 cellsC2C12 skeletal muscle myoblastsP19 murine embryonal carcinoma cellsEmbryonal carcinoma stem cellsAdult mouse tissuesMurine embryonal carcinoma cellsTBX5 transcription factorSkeletal muscle myoblastsEmbryonal carcinoma cellsCarcinoma stem cellsP204 expressionP204 proteinExpression of GATA4Regulatory regionsTBX5 proteinReporter constructsAntisense RNAMuscle myoblastsCardiac myocytesMouse tissuesStem cellsGATA4An engineered VEGF‐activating zinc finger protein transcription factor improves blood flow and limb salvage in advanced‐age mice
Yu J, Lei L, Liang Y, Hinh L, Hickey RP, Huang Y, Liu D, Yeh JL, Rebar E, Case C, Spratt K, Sessa WC, Giordano FJ. An engineered VEGF‐activating zinc finger protein transcription factor improves blood flow and limb salvage in advanced‐age mice. The FASEB Journal 2006, 20: 479-481. PMID: 16423874, DOI: 10.1096/fj.04-3670fje.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAgingAmino Acid SequenceAnimalsBlood Flow VelocityFeasibility StudiesGene Expression RegulationGenes, SyntheticGenetic TherapyGenetic VectorsHindlimbIschemiaLaser-Doppler FlowmetryMiceMice, Inbred C57BLMolecular Sequence DataNeovascularization, PhysiologicProtein EngineeringRecombinant ProteinsRNA, MessengerStructure-Activity RelationshipTranscription FactorsVascular Endothelial Growth Factor AZinc FingersConceptsLimb salvageBlood flowHindlimb ischemiaC57/BL6 micePeripheral vascular diseaseVascular endothelial growth factorPotential clinical utilityEndothelial growth factorExpression of VEGFABL6 miceIschemic limbsVascular diseaseIschemic hindlimbMurine modelClinical utilityVessel countProtein transcription factorsGrowth factorProtein levelsSalvage
2004
Loss of HIF-1α in endothelial cells disrupts a hypoxia-driven VEGF autocrine loop necessary for tumorigenesis
Tang N, Wang L, Esko J, Giordano FJ, Huang Y, Gerber HP, Ferrara N, Johnson RS. Loss of HIF-1α in endothelial cells disrupts a hypoxia-driven VEGF autocrine loop necessary for tumorigenesis. Cancer Cell 2004, 6: 485-495. PMID: 15542432, DOI: 10.1016/j.ccr.2004.09.026.Peer-Reviewed Original ResearchAnimalsCell HypoxiaCell ProliferationCells, CulturedChemotaxisEndothelial CellsGene Expression RegulationHypoxia-Inducible Factor 1, alpha SubunitMiceNeoplasmsNeovascularization, PathologicTranscription FactorsVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2Wound Healing
2002
Induction of angiogenesis in a mouse model using engineered transcription factors
Rebar EJ, Huang Y, Hickey R, Nath AK, Meoli D, Nath S, Chen B, Xu L, Liang Y, Jamieson AC, Zhang L, Spratt SK, Case CC, Wolffe A, Giordano FJ. Induction of angiogenesis in a mouse model using engineered transcription factors. Nature Medicine 2002, 8: 1427-1432. PMID: 12415262, DOI: 10.1038/nm1202-795.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAngiogenesis Inducing AgentsAnimalsDrug DesignGene Expression RegulationGenetic TherapyMiceModels, AnimalMolecular Sequence DataNeovascularization, PhysiologicProtein EngineeringRecombinant ProteinsTranscription FactorsVascular Endothelial Growth Factor AZinc FingersConceptsTranscription factorsEndogenous genesZinc finger protein transcription factorsProtein transcription factorsWhole-organism modelDNA sequencesInduced expressionGenesInduction of angiogenesisZFPExpression of VEGFAProtein VEGFExpressionGrowth factorStimulation of angiogenesisTissue cultureVascular endothelial growth factorExperimental wound healingEndothelial growth factorWound healingNatural arraysAngiogenesisVivoCDNAMouse model