2015
miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice
Goedeke L, Rotllan N, Ramírez CM, Aranda JF, Canfrán-Duque A, Araldi E, Fernández-Hernando A, Langhi C, de Cabo R, Baldán Á, Suárez Y, Fernández-Hernando C. miR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice. Atherosclerosis 2015, 243: 499-509. PMID: 26520906, PMCID: PMC4975922, DOI: 10.1016/j.atherosclerosis.2015.09.033.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAdaptor Proteins, Signal TransducingAnimalsATP Binding Cassette Transporter 1BiomarkersChlorocebus aethiopsCholesterolComputational BiologyCOS CellsDatabases, GeneticDiet, High-FatGene Expression RegulationGene Regulatory NetworksHep G2 CellsHumansLiverMacaca mulattaMaleMice, Inbred C57BLMicroRNAsReceptors, LDLTime FactorsTransfectionTriglyceridesConceptsWild-type miceHepatic lipid levelsMiR-27b expressionLipid levelsHepatic lipidsABCA1 expressionMiR-27bWeeks of treatmentExpression of ABCA1Potential therapeutic targetABCA1 protein levelsCellular cholesterol effluxMiR-27b functionsMiR-27b overexpressionMouse hepatic cellsHepatic LDLRHepatic ABCA1Human hepatic Huh7 cellsHepatic cholesterolWestern dietCardiovascular diseaseTherapeutic administrationLDLR expressionTreatment groupsCholesterol efflux
2013
A Regulatory Role for MicroRNA 33* in Controlling Lipid Metabolism Gene Expression
Goedeke L, Vales-Lara FM, Fenstermaker M, Cirera-Salinas D, Chamorro-Jorganes A, Ramírez CM, Mattison JA, de Cabo R, Suárez Y, Fernández-Hernando C. A Regulatory Role for MicroRNA 33* in Controlling Lipid Metabolism Gene Expression. Molecular And Cellular Biology 2013, 33: 2339-2352. PMID: 23547260, PMCID: PMC3648071, DOI: 10.1128/mcb.01714-12.Peer-Reviewed Original ResearchConceptsMiR-33Gene expressionRegulatory roleTarget gene networkKey transcriptional regulatorTarget gene expressionMetabolism gene expressionIntronic microRNAsHuman hepatic cellsLipid metabolismSterol regulatory element-binding protein 2Transcriptional regulatorsSister strandsGene networksLipid metabolism gene expressionSteady-state levelsHost genesFatty acid metabolismFatty acid oxidationKey enzymeLipid homeostasisPassenger strandMicroRNA-33Functional roleProtein 2
2011
MicroRNA-16 and MicroRNA-424 Regulate Cell-Autonomous Angiogenic Functions in Endothelial Cells via Targeting Vascular Endothelial Growth Factor Receptor-2 and Fibroblast Growth Factor Receptor-1
Chamorro-Jorganes A, Araldi E, Penalva LO, Sandhu D, Fernández-Hernando C, Suárez Y. MicroRNA-16 and MicroRNA-424 Regulate Cell-Autonomous Angiogenic Functions in Endothelial Cells via Targeting Vascular Endothelial Growth Factor Receptor-2 and Fibroblast Growth Factor Receptor-1. Arteriosclerosis Thrombosis And Vascular Biology 2011, 31: 2595-2606. PMID: 21885851, PMCID: PMC3226744, DOI: 10.1161/atvbaha.111.236521.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAnimalsCattleCell MovementCell ProliferationCells, CulturedEndothelium, VascularHumansMiceMice, SCIDMicroRNAsNeovascularization, PhysiologicReceptor, Fibroblast Growth Factor, Type 1Signal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsVEGF receptor 2MiR-424 overexpressionPosttranscriptional regulationGrowth factor receptor 1MiR-424Factor receptor 1MiR-16Vascular endothelial growth factorFibroblast growth factor receptor 1Endothelial cellsMature miR-16Basic fibroblast growth factor (bFGF) treatmentFibroblast growth factor treatmentGrowth factorCellular processesBioinformatics approachPrimary transcriptAbility of ECsDownstream componentsTarget genesVascular endothelial growth factor receptor 2Receptor 1Endothelial growth factor receptor 2Cord formationLentiviral overexpressionMicroRNA-758 Regulates Cholesterol Efflux Through Posttranscriptional Repression of ATP-Binding Cassette Transporter A1
Ramirez CM, Dávalos A, Goedeke L, Salerno AG, Warrier N, Cirera-Salinas D, Suárez Y, Fernández-Hernando C. MicroRNA-758 Regulates Cholesterol Efflux Through Posttranscriptional Repression of ATP-Binding Cassette Transporter A1. Arteriosclerosis Thrombosis And Vascular Biology 2011, 31: 2707-2714. PMID: 21885853, PMCID: PMC3298756, DOI: 10.1161/atvbaha.111.232066.Peer-Reviewed Original ResearchConceptsATP-binding cassette transporter A1Cassette transporter A1Posttranscriptional regulationCellular cholesterol effluxUnbiased genome-wide screenMiR-758Cholesterol effluxGenome-wide screenExpression of ABCA1Cholesterol-loaded macrophagesIntracellular cholesterol accumulationPosttranscriptional repressionNovel miRNABioinformatics analysisMouse cellsMajor regulatorHuman cellsLuciferase reporterMacrophage cholesterol effluxMouse peritoneal macrophagesPeritoneal macrophagesABCA1 geneABCA1 expressionQuantitative real-time reverse transcription-polymerase chain reactionHigh-density lipoprotein levels