2015
VEGF-Induced Expression of miR-17–92 Cluster in Endothelial Cells Is Mediated by ERK/ELK1 Activation and Regulates Angiogenesis
Chamorro-Jorganes A, Lee MY, Araldi E, Landskroner-Eiger S, Fernández-Fuertes M, Sahraei M, del Rey M, van Solingen C, Yu J, Fernández-Hernando C, Sessa WC, Suárez Y. VEGF-Induced Expression of miR-17–92 Cluster in Endothelial Cells Is Mediated by ERK/ELK1 Activation and Regulates Angiogenesis. Circulation Research 2015, 118: 38-47. PMID: 26472816, PMCID: PMC4703066, DOI: 10.1161/circresaha.115.307408.Peer-Reviewed Original ResearchConceptsMiR-17Elk1 activationEndothelial angiogenic functionEC proliferationRegulation of angiogenesisTranscription activationTranscriptional programsGenetic evidenceCluster expressionTumor angiogenesisAngiogenic sproutingVEGF stimulationRescue experimentsRetinal angiogenesisRegulate angiogenesisLines of evidenceEndothelial cell functionAngiogenic switchPhysiological retinal angiogenesisAngiogenic functionDevelopmental retinal angiogenesisCell functionTumor developmentRegulationCrucial mediator
2014
Autoregulation of glypican-1 by intronic microRNA-149 fine tunes the angiogenic response to FGF2 in human endothelial cells
Chamorro-Jorganes A, Araldi E, Rotllan N, Cirera-Salinas D, Suárez Y. Autoregulation of glypican-1 by intronic microRNA-149 fine tunes the angiogenic response to FGF2 in human endothelial cells. Journal Of Cell Science 2014, 127: 1169-1178. PMID: 24463821, PMCID: PMC3953812, DOI: 10.1242/jcs.130518.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarcinoma, Lewis LungCells, CulturedFibroblast Growth Factor 2Gene ExpressionGlypicansHuman Umbilical Vein Endothelial CellsHumansMaleMiceMicroRNAsNeoplasm TransplantationNeovascularization, PathologicNeovascularization, PhysiologicReceptor, Fibroblast Growth Factor, Type 1RNA InterferenceSignal Transduction
2012
miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish
Stahlhut C, Suárez Y, Lu J, Mishima Y, Giraldez AJ. miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish. Development 2012, 139: 4356-4365. PMID: 23132244, PMCID: PMC3509730, DOI: 10.1242/dev.083774.Peer-Reviewed Original ResearchConceptsMiR-1/206Post-transcriptional modulatorsMiRNA-target interactionsMiR-1Appropriate physiological responsesRegulation of VEGFAZebrafish developmentEmbryonic developmentTarget protectorNovel functionPrecise regulationGene expressionMorphogenetic activityDevelopmental angiogenesisPutative targetsRegulate angiogenesisEssential processMiR-206Physiological responsesCellular communicationVEGFA expressionGrowth factorVascular endothelial growth factorExpressionAngiogenesis
2011
MicroRNA-16 and MicroRNA-424 Regulate Cell-Autonomous Angiogenic Functions in Endothelial Cells via Targeting Vascular Endothelial Growth Factor Receptor-2 and Fibroblast Growth Factor Receptor-1
Chamorro-Jorganes A, Araldi E, Penalva LO, Sandhu D, Fernández-Hernando C, Suárez Y. MicroRNA-16 and MicroRNA-424 Regulate Cell-Autonomous Angiogenic Functions in Endothelial Cells via Targeting Vascular Endothelial Growth Factor Receptor-2 and Fibroblast Growth Factor Receptor-1. Arteriosclerosis Thrombosis And Vascular Biology 2011, 31: 2595-2606. PMID: 21885851, PMCID: PMC3226744, DOI: 10.1161/atvbaha.111.236521.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAnimalsCattleCell MovementCell ProliferationCells, CulturedEndothelium, VascularHumansMiceMice, SCIDMicroRNAsNeovascularization, PhysiologicReceptor, Fibroblast Growth Factor, Type 1Signal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsVEGF receptor 2MiR-424 overexpressionPosttranscriptional regulationGrowth factor receptor 1MiR-424Factor receptor 1MiR-16Vascular endothelial growth factorFibroblast growth factor receptor 1Endothelial cellsMature miR-16Basic fibroblast growth factor (bFGF) treatmentFibroblast growth factor treatmentGrowth factorCellular processesBioinformatics approachPrimary transcriptAbility of ECsDownstream componentsTarget genesVascular endothelial growth factor receptor 2Receptor 1Endothelial growth factor receptor 2Cord formationLentiviral overexpression
2010
Microregulation of Plaque Neovascularization
Suárez Y. Microregulation of Plaque Neovascularization. Arteriosclerosis Thrombosis And Vascular Biology 2010, 30: 1500-1501. PMID: 20631347, DOI: 10.1161/atvbaha.110.209551.Commentaries, Editorials and Letters
2009
MicroRNAs As Novel Regulators of Angiogenesis
Suárez Y, Sessa WC. MicroRNAs As Novel Regulators of Angiogenesis. Circulation Research 2009, 104: 442-454. PMID: 19246688, PMCID: PMC2760389, DOI: 10.1161/circresaha.108.191270.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenic ProteinsAnimalsCardiovascular DiseasesEndothelial CellsGene Expression RegulationGenetic TherapyHumansMicroRNAsNeovascularization, PathologicNeovascularization, PhysiologicConceptsInvolvement of miRNAsShort noncoding RNAsPosttranscriptional regulationNoncoding RNAsNovel regulatorKey regulatorNegative regulatorGene expressionAspects of developmentNew blood vesselsRegulatorVascular biologyCurrent experimental evidencePotential therapeutic applicationsMiRNAsMicroRNAsAngiogenic processEndothelial cellsRegulationAbnormal angiogenesisTherapeutic applicationsAngiogenesisRNABiologyHomeostasis
2008
Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis
Suárez Y, Fernández-Hernando C, Yu J, Gerber SA, Harrison KD, Pober JS, Iruela-Arispe ML, Merkenschlager M, Sessa WC. Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 14082-14087. PMID: 18779589, PMCID: PMC2544582, DOI: 10.1073/pnas.0804597105.Peer-Reviewed Original ResearchConceptsEndothelial miRNAsPostnatal angiogenesisMiR-17Posttranscriptional gene regulationLoss of DicerGeneration of miRNAsExpression of miRNAsCluster miR-17Cell-specific inactivationGene regulationAngiogenic responseEndothelial microRNAsInduced expressionMiRNAsAspects of developmentEndothelial cell proliferationVariety of stimuliCell proliferationDicerThrombospondin-1Exogenous VEGFMicroRNAsExpressionEndothelial cellsRegulationProhibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence
Schleicher M, Shepherd BR, Suarez Y, Fernandez-Hernando C, Yu J, Pan Y, Acevedo LM, Shadel GS, Sessa WC. Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence. Journal Of Cell Biology 2008, 180: 101-112. PMID: 18195103, PMCID: PMC2213620, DOI: 10.1083/jcb.200706072.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCells, CulturedCellular SenescenceCytoskeletonElectron Transport Complex IEndothelial CellsEndothelium, VascularHumansMiceMice, Inbred StrainsMitochondriaMitochondrial MembranesNeovascularization, PhysiologicNeuropeptidesPhosphatidylinositol 3-KinasesProhibitinsProto-Oncogene Proteins c-aktRac GTP-Binding ProteinsRac1 GTP-Binding ProteinReactive Oxygen SpeciesRepressor ProteinsSignal TransductionConceptsProhibitin 1Mitochondrial functionKnockdown of PHB1Inner mitochondrial membraneEndothelial cell motilityEndothelial cellsCytoskeletal rearrangementsMitochondrial membraneVivo angiogenesis assaysCell motilityAngiogenic capacityCellular senescenceReactive oxygen speciesMitochondrial productionFunctional blood vesselsImportant regulatorSenescenceAngiogenesis assayTube formationOxygen speciesVascular homeostasisCellsVascular systemRac1Yeast
2006
Vascularization and engraftment of a human skin substitute using circulating progenitor cell‐derived endothelial cells
Shepherd BR, Enis DR, Wang F, Suarez Y, Pober JS, Schechner JS, Shepherd B, Enis D, Wang F, Suarez Y, Pober J, Scheduier J. Vascularization and engraftment of a human skin substitute using circulating progenitor cell‐derived endothelial cells. The FASEB Journal 2006, 20: 1739-1741. PMID: 16807367, DOI: 10.1096/fj.05-5682fje.Peer-Reviewed Original Research