2020
Cav-1 (Caveolin-1) Deficiency Increases Autophagy in the Endothelium and Attenuates Vascular Inflammation and Atherosclerosis
Zhang X, Ramírez CM, Aryal B, Madrigal-Matute J, Liu X, Diaz A, Torrecilla-Parra M, Suárez Y, Cuervo AM, Sessa WC, Fernández-Hernando C. Cav-1 (Caveolin-1) Deficiency Increases Autophagy in the Endothelium and Attenuates Vascular Inflammation and Atherosclerosis. Arteriosclerosis Thrombosis And Vascular Biology 2020, 40: 1510-1522. PMID: 32349535, PMCID: PMC7253189, DOI: 10.1161/atvbaha.120.314291.Peer-Reviewed Original ResearchConceptsCav-1 deficiencyCav-1-deficient miceCav-1Autophagic fluxCholesterol-rich membrane domainsCav-1 interactsATG5-ATG12 complexEndothelial Cav-1 expressionRegulation of autophagyNovel molecular mechanismExtracellular matrix remodelingAutophagosome componentsMembrane domainsLipid raftsAutophagosome formationPlasma membraneCav-1 expressionMolecular mechanismsLDL transcytosisCellular localizationImportant regulatorAutophagyAutophagy contributesRelevant regulatorMatrix remodeling
2016
SREBP-1c/MicroRNA 33b Genomic Loci Control Adipocyte Differentiation
Price NL, Holtrup B, Kwei SL, Wabitsch M, Rodeheffer M, Bianchini L, Suárez Y, Fernández-Hernando C. SREBP-1c/MicroRNA 33b Genomic Loci Control Adipocyte Differentiation. Molecular And Cellular Biology 2016, 36: 1180-1193. PMID: 26830228, PMCID: PMC4800797, DOI: 10.1128/mcb.00745-15.Peer-Reviewed Original ResearchConceptsWhite adipose tissueCyclin-dependent kinase 6MiR-33bSREBP-1Adipocyte differentiationReceptor-γ target genesPeroxisome proliferator-activated receptor-γ target genesDevelopment of obesityStandard mouse modelSterol regulatory element-binding protein 2Lipid droplet formationLipid droplet accumulationIntronic microRNAsHost genesTarget genesMouse modelKinase 6Adipose tissueMetabolic diseasesNovel roleImportant regulatorHuman preadipocytesDroplet accumulationVivo assessmentProtein 2
2015
The miR-199–dynamin regulatory axis controls receptor-mediated endocytosis
Aranda JF, Canfrán-Duque A, Goedeke L, Suárez Y, Fernández-Hernando C. The miR-199–dynamin regulatory axis controls receptor-mediated endocytosis. Journal Of Cell Science 2015, 128: 3197-3209. PMID: 26163491, PMCID: PMC4582188, DOI: 10.1242/jcs.165233.Peer-Reviewed Original ResearchConceptsClathrin heavy chainReceptor-mediated endocytosisIntracellular traffickingLow-density lipoprotein receptorGene expressionMiR-199aSmall non-coding RNAsNon-coding RNAsTarget gene expressionDynamin genesEukaryotic cellsHuman cell linesEndocytic transportGTPase familyCav-1 expressionUnexpected layerCaveolin-1Intronic sequencesIntracellular transportPhysiological processesEndocytosisImportant regulatorMiR-199bCell linesGenes
2008
Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence
Schleicher M, Shepherd BR, Suarez Y, Fernandez-Hernando C, Yu J, Pan Y, Acevedo LM, Shadel GS, Sessa WC. Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence. Journal Of Cell Biology 2008, 180: 101-112. PMID: 18195103, PMCID: PMC2213620, DOI: 10.1083/jcb.200706072.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCells, CulturedCellular SenescenceCytoskeletonElectron Transport Complex IEndothelial CellsEndothelium, VascularHumansMiceMice, Inbred StrainsMitochondriaMitochondrial MembranesNeovascularization, PhysiologicNeuropeptidesPhosphatidylinositol 3-KinasesProhibitinsProto-Oncogene Proteins c-aktRac GTP-Binding ProteinsRac1 GTP-Binding ProteinReactive Oxygen SpeciesRepressor ProteinsSignal TransductionConceptsProhibitin 1Mitochondrial functionKnockdown of PHB1Inner mitochondrial membraneEndothelial cell motilityEndothelial cellsCytoskeletal rearrangementsMitochondrial membraneVivo angiogenesis assaysCell motilityAngiogenic capacityCellular senescenceReactive oxygen speciesMitochondrial productionFunctional blood vesselsImportant regulatorSenescenceAngiogenesis assayTube formationOxygen speciesVascular homeostasisCellsVascular systemRac1Yeast