2016
Akt‐mediated foxo1 inhibition is required for liver regeneration
Pauta M, Rotllan N, Fernández-Hernando A, Langhi C, Ribera J, Lu M, Boix L, Bruix J, Jimenez W, Suárez Y, Ford DA, Baldán A, Birnbaum MJ, Morales-Ruiz M, Fernández-Hernando C. Akt‐mediated foxo1 inhibition is required for liver regeneration. Hepatology 2016, 63: 1660-1674. PMID: 26473496, PMCID: PMC5177729, DOI: 10.1002/hep.28286.Peer-Reviewed Original ResearchConceptsAkt/protein kinase BCellular eventsProtein kinase BAkt2-deficient miceAbsence of Akt1Lipid droplet formationContribution of AktAkt2-null miceLiver regenerationAbnormal cellular eventsTranscription factorsAKT-FOXO1Kinase BLiver-specific deletionSuccessful liver regenerationPartial hepatectomyHepatic regenerative capabilityAKT1Chronic liver diseaseFOXO1 inhibitionCell proliferationEssential roleImpaired liver regenerationIntracellular mediatorsEfficient liver regenerationMicroRNAs as regulators of endothelial cell functions in cardiometabolic diseases
Araldi E, Suárez Y. MicroRNAs as regulators of endothelial cell functions in cardiometabolic diseases. Biochimica Et Biophysica Acta 2016, 1861: 2094-2103. PMID: 26825686, PMCID: PMC5039046, DOI: 10.1016/j.bbalip.2016.01.013.Peer-Reviewed Original ResearchConceptsSmall non-coding RNAsLipid/energy metabolismCarlos Fernández-HernandoRegulation of ECNon-coding RNAsRole of miRNAsEndothelial cellsYajaira SuárezTissue homeostasisCell deathEndothelial cell functionEnvironmental stimuliEnergy metabolismMicroRNAsCell proliferationImportant functionsPotential therapeutic applicationsCell functionMiRNAsDifferent cardiometabolic diseasesMetabolic imbalanceEC dysfunctionTherapeutic applicationsPresent reviewRelated diseases
2015
Disruption of the mevalonate pathway induces dNTP depletion and DNA damage
Sánchez C, Martín J, Jin JS, Dávalos A, Zhang W, de la Peña G, Martínez-Botas J, Rodríguez-Acebes S, Suárez Y, Hazen MJ, Gómez-Coronado D, Busto R, Cheng YC, Lasunción MA. Disruption of the mevalonate pathway induces dNTP depletion and DNA damage. Biochimica Et Biophysica Acta 2015, 1851: 1240-1253. PMID: 26055626, DOI: 10.1016/j.bbalip.2015.06.001.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateCarboxy-LyasesCell Cycle CheckpointsCell Line, TumorCell ProliferationCheckpoint Kinase 1DeoxyribonucleosidesDNA DamageDNA ReplicationGene Expression RegulationHalogenationHemiterpenesHistonesHL-60 CellsHumansLymphocytesMevalonic AcidOrganophosphorus CompoundsProtein KinasesRNA, Small InterferingSignal TransductionConceptsMevalonate diphosphate decarboxylaseDiphosphate decarboxylaseCell cycle progressionDNA replicationCycle progressionMevalonate pathwayDNA damageDNA damage responseNon-sterol isoprenoidsCell proliferationInhibition of Chk1Cholesterol biosynthesis pathwayMassive cell deathSubsequent DNA damageΓ-H2AX formationCell cycle arrestReplication stressBiosynthesis pathwayΓ-H2AX fociChk1 activationDamage responseIsopentenyl diphosphateMitosis completionCell divisionDNTP depletion
2012
Mir-33 regulates cell proliferation and cell cycle progression
Cirera-Salinas D, Pauta M, Allen RM, Salerno AG, Ramírez CM, Chamorro-Jorganes A, Wanschel AC, Lasuncion MA, Morales-Ruiz M, Suarez Y, Baldan A, Esplugues E, Fernández-Hernando C. Mir-33 regulates cell proliferation and cell cycle progression. Cell Cycle 2012, 11: 922-933. PMID: 22333591, PMCID: PMC3323796, DOI: 10.4161/cc.11.5.19421.Peer-Reviewed Original ResearchConceptsCell cycle progressionCyclin-dependent kinase 6Cycle progressionCell proliferationCell cycle regulationMiR-33Expression of genesCyclin D1Cell cycle arrestSREBP genesCycle regulationFatty acid metabolismHost genesPosttranscriptional levelGene expressionIntronic sequencesKinase 6Cellular growthCritical regulatorCycle arrestCellular levelLiver regenerationGenesMiR-33 expressionAcid metabolism
2008
Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis
Suárez Y, Fernández-Hernando C, Yu J, Gerber SA, Harrison KD, Pober JS, Iruela-Arispe ML, Merkenschlager M, Sessa WC. Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 14082-14087. PMID: 18779589, PMCID: PMC2544582, DOI: 10.1073/pnas.0804597105.Peer-Reviewed Original ResearchConceptsEndothelial miRNAsPostnatal angiogenesisMiR-17Posttranscriptional gene regulationLoss of DicerGeneration of miRNAsExpression of miRNAsCluster miR-17Cell-specific inactivationGene regulationAngiogenic responseEndothelial microRNAsInduced expressionMiRNAsAspects of developmentEndothelial cell proliferationVariety of stimuliCell proliferationDicerThrombospondin-1Exogenous VEGFMicroRNAsExpressionEndothelial cellsRegulation
2005
Sterol stringency of proliferation and cell cycle progression in human cells
Suárez Y, Fernández C, Ledo B, Martín M, Gómez-Coronado D, Lasunción MA. Sterol stringency of proliferation and cell cycle progression in human cells. Biochimica Et Biophysica Acta 2005, 1734: 203-213. PMID: 15904877, DOI: 10.1016/j.bbalip.2005.02.003.Peer-Reviewed Original ResearchConceptsCell cycle progressionCycle progressionG2/M phaseMammalian cellsCell proliferationCell growthM phaseRegulator of proliferationSterol response elementCholesterol-free mediumPlasma membraneCell cycleResponse elementIsoprenoid derivativesStructural roleHuman cellsCholesterol biosynthesisSKF 104976Sterol analoguesDifferent sterolsHuman leukemiaCell processesCholesterol analoguesHL-60Concentration-dependent manner
2002
A double mutant [N543H+2393del9] allele in the LDL receptor gene in familial hypercholesterolemia: effect on plasma cholesterol levels and cardiovascular disease
Castillo S, Reyes G, Tejedor D, Mozas P, Suarez Y, Lasuncion M, Cenarro A, Civeira F, Alonso R, Mata P, Pocovi M, Group of FH O. A double mutant [N543H+2393del9] allele in the LDL receptor gene in familial hypercholesterolemia: effect on plasma cholesterol levels and cardiovascular disease. Human Mutation 2002, 20: 477-477. PMID: 12442279, DOI: 10.1002/humu.9087.Peer-Reviewed Original ResearchConceptsDouble mutant alleleLDL receptor geneFamilial hypercholesterolemiaHomozygous patientsReceptor geneSpanish FH patientsCholesterol-lowering treatmentLDL cholesterol reductionPlasma cholesterol levelsAbility of LDLMitogen-stimulated lymphocytesCholesterol levelsCholesterol reductionFH patientsCardiovascular diseasePatientsHomozygous FHHeterozygous patientsUnrelated patientsCytometric analysisHypercholesterolemiaLDL bindingDefective LDL bindingCell proliferationGenetic disordersDifferential effects of ergosterol and cholesterol on Cdk1 activation and SRE‐driven transcription
Suárez Y, Fernández C, Ledo B, Ferruelo AJ, Martín M, Vega MA, Gómez‐Coronado D, Lasunción MA. Differential effects of ergosterol and cholesterol on Cdk1 activation and SRE‐driven transcription. The FEBS Journal 2002, 269: 1761-1771. PMID: 11895447, DOI: 10.1046/j.1432-1327.2002.02822.x.Peer-Reviewed Original ResearchConceptsHuman cellsCdk1 activationCell cycle machineryCell membrane formationCell cycle progressionCholesterol-free mediumCell cycle arrestG2/M phaseSpecific regulatorsCycle machineryGene constructsYeast sterolCycle progressionCell cycleCell proliferation inhibitionCycle arrestAction of cholesterolUCN-01Cell growthCyclin B1 expressionSKF 104976Cholesterol homeostasisM phaseMembrane formationCell proliferation
2001
Dose-dependent effects of lovastatin on cell cycle progression. Distinct requirement of cholesterol and non-sterol mevalonate derivatives
Martı́nez-Botas J, Ferruelo A, Suárez Y, Fernández C, Gómez-Coronado D, Lasunción M. Dose-dependent effects of lovastatin on cell cycle progression. Distinct requirement of cholesterol and non-sterol mevalonate derivatives. Biochimica Et Biophysica Acta 2001, 1532: 185-194. PMID: 11470239, DOI: 10.1016/s1388-1981(01)00125-1.Peer-Reviewed Original ResearchConceptsCell proliferationLow-density lipoprotein cholesterolCell cycle progressionDose-dependent effectCell cycle distributionCell cycleCycle progressionLipoprotein cholesterolConcentrations of lovastatinCholesterol supplyCycle distributionCholesterolLovastatinHuman cell linesCell linesCholesterol biosynthesisCholesterol-free mediumNormal cell cyclingM phaseProgressionProliferationPresent studyHL-60Mevalonate derivativesCell cycling