2019
Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells
Dong MB, Wang G, Chow RD, Ye L, Zhu L, Dai X, Park JJ, Kim HR, Errami Y, Guzman CD, Zhou X, Chen KY, Renauer PA, Du Y, Shen J, Lam SZ, Zhou JJ, Lannin DR, Herbst RS, Chen S. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell 2019, 178: 1189-1204.e23. PMID: 31442407, PMCID: PMC6719679, DOI: 10.1016/j.cell.2019.07.044.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBreast NeoplasmsCD8-Positive T-LymphocytesCell Line, TumorClustered Regularly Interspaced Short Palindromic RepeatsCytokinesFemaleHumansImmunologic MemoryImmunotherapyMaleMiceMice, KnockoutNF-kappa BProgrammed Cell Death 1 ReceptorRNA HelicasesRNA, Guide, CRISPR-Cas SystemsTranscriptomeConceptsCRISPR screensTarget discoveryGenome-scale CRISPR screensCD8 TRNA helicase DHX37Vivo CRISPR screensGenetic screenGenome scaleTranscriptomic profilingBiochemical interrogationAntigen-specific CD8 TAnti-tumor immune responseFunctional regulatorTriple-negative breast cancerDHX37Essential roleTim-3PD-1Cytokine productionTumor infiltrationImmunotherapy targetImmunotherapy settingsRegulatorBreast cancerT cells
2017
Analysis of immunological mechanisms exerted by HBsAg-HBIG therapeutic vaccine combined with Adefovir in chronic hepatitis B patients
Zhou C, Li C, Gong G, Wang S, Zhang J, Xu D, Guo L, Ren H, Xu M, Xie Q, Pan C, Xu J, Hu Z, Geng S, Zhou X, Wang X, Zhou X, Mi H, Zhao G, Yu W, Wen Y, Huang L, Wang X, Wang B. Analysis of immunological mechanisms exerted by HBsAg-HBIG therapeutic vaccine combined with Adefovir in chronic hepatitis B patients. Human Vaccines & Immunotherapeutics 2017, 13: 1989-1996. PMID: 28665747, PMCID: PMC5612521, DOI: 10.1080/21645515.2017.1335840.Peer-Reviewed Original ResearchMeSH KeywordsAdenineAdjuvants, ImmunologicAdultAntigen-Antibody ComplexCombined Modality TherapyFemaleHepatitis B Surface AntigensHepatitis B VaccinesHepatitis B, ChronicHumansInterleukin-10Interleukin-17Interleukin-2MaleOrganophosphonatesTh1 CellsTh2 CellsTransforming Growth Factor betaTumor Necrosis Factor-alphaYoung AdultConceptsChronic hepatitis B patientsHepatitis B patientsT cellsImmunized groupsB patientsImmunological mechanismsTherapeutic vaccinesHost-specific immune responseIL-2 levelsSero-conversion rateSpecific immune responseEarly inflammatory responseCHB patientsTc17 cellsTreg cellsCytokine profileIL-17AIL-10Saline groupCell CD4More injectionsAdjuvant controlsClinical trialsInflammatory responseNormal saline