2014
In Vitro Culture Increases the Frequency of Stochastic Epigenetic Errors at Imprinted Genes in Placental Tissues from Mouse Concepti Produced Through Assisted Reproductive Technologies1
de Waal E, Mak W, Calhoun S, Stein P, Ord T, Krapp C, Coutifaris C, Schultz RM, Bartolomei MS. In Vitro Culture Increases the Frequency of Stochastic Epigenetic Errors at Imprinted Genes in Placental Tissues from Mouse Concepti Produced Through Assisted Reproductive Technologies1. Biology Of Reproduction 2014, 90: 22, 1-12. PMID: 24337315, PMCID: PMC4076403, DOI: 10.1095/biolreprod.113.114785.Peer-Reviewed Original ResearchConceptsEpigenetic errorsEpigenetic defectsART-conceived offspringImprinted genesMammalian embryosDNA methylationEpigenetic profilesPreimplantation developmentEpigenetic abnormalitiesAssisted Reproductive TechnologyMammalian speciesMouse conceptiMouse embryosExpression profilesEmbryonic tissuesAbnormal methylationGenesEmbryosPlacental tissueIVF embryosVitro CultureMethylationHigh oxygen tensionLow birth weightMillions of couples
2013
Loss of DNMT1o Disrupts Imprinted X Chromosome Inactivation and Accentuates Placental Defects in Females
McGraw S, Oakes CC, Martel J, Cirio MC, de Zeeuw P, Mak W, Plass C, Bartolomei MS, Chaillet JR, Trasler JM. Loss of DNMT1o Disrupts Imprinted X Chromosome Inactivation and Accentuates Placental Defects in Females. PLOS Genetics 2013, 9: e1003873. PMID: 24278026, PMCID: PMC3836718, DOI: 10.1371/journal.pgen.1003873.Peer-Reviewed Original ResearchConceptsImprinted X-chromosome inactivationX-chromosome inactivationChromosome inactivationImprinted X inactivationDNA methylation eventsX-inactivation centerDNA methylation patternsKey regulatory regionsGenomic imprintsMethylation maintenanceGenomic imprintingImprinted lociFemale blastocystsMethylation eventsExtraembryonic tissuesBiallelic expressionMethylation patternsRegulatory regionsPreimplantation developmentAffected lociX chromosomeX inactivationMouse embryosDNA hypomethylationPreimplantation embryos
2011
National study of factors influencing assisted reproductive technology outcomes with male factor infertility
Nangia AK, Luke B, Smith JF, Mak W, Stern JE, Group T. National study of factors influencing assisted reproductive technology outcomes with male factor infertility. Fertility And Sterility 2011, 96: 609-614. PMID: 21733503, DOI: 10.1016/j.fertnstert.2011.06.026.Peer-Reviewed Original ResearchConceptsClinical intrauterine gestationMale factor infertilityFactor infertilityICSI cyclesTubal ligationAssisted Reproductive Technology Clinic Outcome Reporting System databaseLB rateMale factor infertility casesHistoric cohort studyReproductive technology cyclesClinic-based dataReproductive technology outcomesMultivariate logistic regressionReporting System databaseCohort studyIntrauterine gestationICSI outcomesInfertility casesWorse outcomesOdds ratioMAIN OUTCOMESperm injectionInfertilityLogistic regressionSperm collectionDisruption of a conserved region of Xist exon 1 impairs Xist RNA localisation and X-linked gene silencing during random and imprinted X chromosome inactivation.
Senner CE, Nesterova TB, Norton S, Dewchand H, Godwin J, Mak W, Brockdorff N. Disruption of a conserved region of Xist exon 1 impairs Xist RNA localisation and X-linked gene silencing during random and imprinted X chromosome inactivation. Development (Cambridge, England) 2011, 138: 1541-50. PMID: 21389056, PMCID: PMC3062423, DOI: 10.1242/dev.056812.Peer-Reviewed Original Research
2009
Polycystic ovarian syndrome and the risk of cardiovascular disease and thrombosis.
Mak W, Dokras A. Polycystic ovarian syndrome and the risk of cardiovascular disease and thrombosis. Seminars In Thrombosis And Hemostasis 2009, 35: 613-20. PMID: 20013528, DOI: 10.1055/s-0029-1242715.Peer-Reviewed Original Research
2004
Reactivation of the paternal X chromosome in early mouse embryos.
Mak W, Nesterova TB, de Napoles M, Appanah R, Yamanaka S, Otte AP, Brockdorff N. Reactivation of the paternal X chromosome in early mouse embryos. Science (New York, N.Y.) 2004, 303: 666-9. PMID: 14752160, DOI: 10.1126/science.1092674.Peer-Reviewed Original Research
2002
Mitotically stable association of polycomb group proteins eed and enx1 with the inactive x chromosome in trophoblast stem cells.
Mak W, Baxter J, Silva J, Newall AE, Otte AP, Brockdorff N. Mitotically stable association of polycomb group proteins eed and enx1 with the inactive x chromosome in trophoblast stem cells. Current Biology : CB 2002, 12: 1016-20. PMID: 12123576.Peer-Reviewed Original Research