2016
Discovery of a Coregulatory Interaction between Kaposi's Sarcoma-Associated Herpesvirus ORF45 and the Viral Protein Kinase ORF36
Avey D, Tepper S, Pifer B, Bahga A, Williams H, Gillen J, Li W, Ogden S, Zhu F. Discovery of a Coregulatory Interaction between Kaposi's Sarcoma-Associated Herpesvirus ORF45 and the Viral Protein Kinase ORF36. Journal Of Virology 2016, 90: 5953-5964. PMID: 27099309, PMCID: PMC4907238, DOI: 10.1128/jvi.00516-16.Peer-Reviewed Original ResearchConceptsKaposi's sarcoma-associated herpesvirusKSHV ORF36Viral protein kinaseSarcoma-associated herpesvirusProtein kinaseKSHV lytic replicationKaposi's Sarcoma-Associated Herpesvirus ORF45Infectious progeny virionsHerpesvirus protein kinasesProgeny virionsLytic replicationKinase-dead mutantBacterial artificial chromosome mutagenesisBasic N-terminusCoregulatory interactionsChromosome mutagenesisORF45 proteinViral life cycleAcidic patchKSHV proteinsORF36Human tumor virusKinase activityORF45N-terminus
2015
Phosphoproteomic Analysis of KSHV-Infected Cells Reveals Roles of ORF45-Activated RSK during Lytic Replication
Avey D, Tepper S, Li W, Turpin Z, Zhu F. Phosphoproteomic Analysis of KSHV-Infected Cells Reveals Roles of ORF45-Activated RSK during Lytic Replication. PLOS Pathogens 2015, 11: e1004993. PMID: 26133373, PMCID: PMC4489790, DOI: 10.1371/journal.ppat.1004993.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCell LineGene Expression Regulation, ViralGene Knockout TechniquesHerpesviridae InfectionsHerpesvirus 8, HumanHost-Parasite InteractionsHumansImmediate-Early ProteinsImmunoprecipitationMass SpectrometryPhosphorylationProteomicsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRibosomal Protein S6 Kinases, 90-kDaTransfectionVirus ReplicationConceptsKaposi's Sarcoma-Associated HerpesvirusKSHV lytic replicationProgeny virion productionKSHV ORF45Putative substratesRSK activityExtracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK) pathwayGene expressionEfficient KSHV lytic replicationLytic replicationP90 ribosomal S6 kinaseMitogen-activated protein kinase pathwayRSK-dependent phosphorylationHost cell machineryRibosomal S6 kinaseProtein kinase pathwayVirion productionActivation of RSKComplex secondary structureKSHV lytic gene expressionTranscriptional regulatorsDifferential phosphorylationLytic gene expressionCellular genesCell machinery