2019
Sirtuin 6 Attenuates Kaposi's Sarcoma-Associated Herpesvirus Reactivation by Suppressing Ori-Lyt Activity and Expression of RTA
Hu M, Armstrong N, Seto E, Li W, Zhu F, Wang P, Tang Q. Sirtuin 6 Attenuates Kaposi's Sarcoma-Associated Herpesvirus Reactivation by Suppressing Ori-Lyt Activity and Expression of RTA. Journal Of Virology 2019, 93: 10.1128/jvi.02200-18. PMID: 30651359, PMCID: PMC6430549, DOI: 10.1128/jvi.02200-18.Peer-Reviewed Original ResearchMeSH KeywordsCell LineCell Line, TumorDNA ReplicationDNA, ViralGene Expression Regulation, ViralHEK293 CellsHerpesvirus 8, HumanHumansImmediate-Early ProteinsNuclear ProteinsPromoter Regions, GeneticRepressor ProteinsRNA, Small InterferingSarcoma, KaposiSirtuinsTrans-ActivatorsViral ProteinsVirus LatencyVirus ReplicationConceptsKaposi's sarcoma-associated herpesvirusViral DNA replicationReactivation of KSHVSarcoma-associated herpesvirusDNA replicationGene expressionViral gene expressionKSHV promotersHistone deacetylasesPromoter activityKSHV reactivationKaposi's Sarcoma-Associated Herpesvirus ReactivationCell linesDepletion of SIRT6KSHV gene expressionChromatin immunoprecipitation assaysExpression of RTAViral protein productionReduced gene expressionCellular regulatorsImmunoprecipitation assaysOverexpression of SIRT6Multiple promotersKSHV genomeOri-Lyt
2015
A Survey of the Interactome of Kaposi's Sarcoma-Associated Herpesvirus ORF45 Revealed Its Binding to Viral ORF33 and Cellular USP7, Resulting in Stabilization of ORF33 That Is Required for Production of Progeny Viruses
Gillen J, Li W, Liang Q, Avey D, Wu J, Wu F, Myoung J, Zhu F. A Survey of the Interactome of Kaposi's Sarcoma-Associated Herpesvirus ORF45 Revealed Its Binding to Viral ORF33 and Cellular USP7, Resulting in Stabilization of ORF33 That Is Required for Production of Progeny Viruses. Journal Of Virology 2015, 89: 4918-4931. PMID: 25694600, PMCID: PMC4403494, DOI: 10.1128/jvi.02925-14.Peer-Reviewed Original ResearchConceptsUbiquitin-specific protease 7Kaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusKSHV lytic replicationORF45 proteinKaposi's Sarcoma-Associated Herpesvirus ORF45Lytic replicationCarboxyl-terminal 19 amino acidsKSHV ORF45Copurified proteinsConsensus motifMultifunctional proteinKinase proteinInteractomeCarboxyl terminusKSHV genomeORF45Tegument proteinsWeight complexesProtein accumulationORF33Progeny virionsAmino acidsHuman cancersProtein
2014
Activation of p90 Ribosomal S6 Kinases by ORF45 of Kaposi's Sarcoma-Associated Herpesvirus Is Critical for Optimal Production of Infectious Viruses
Fu B, Kuang E, Li W, Avey D, Li X, Turpin Z, Valdes A, Brulois K, Myoung J, Zhu F. Activation of p90 Ribosomal S6 Kinases by ORF45 of Kaposi's Sarcoma-Associated Herpesvirus Is Critical for Optimal Production of Infectious Viruses. Journal Of Virology 2014, 89: 195-207. PMID: 25320298, PMCID: PMC4301102, DOI: 10.1128/jvi.01937-14.Peer-Reviewed Original ResearchConceptsRibosomal S6 kinaseP90 ribosomal S6 kinaseKaposi's sarcoma-associated herpesvirusViral gene expressionKSHV lytic replicationSarcoma-associated herpesvirusRSK activationS6 kinaseGene expressionVirus-infected cellsLytic reactivationWild-type virus-infected cellsMutant virus-infected cellsBacterial artificial chromosomeAlanine-scanning mutagenesisLytic replicationCritical rolePhosphoproteomic profilesArtificial chromosomesKinase ERKScanning mutagenesisProgeny viral particlesKSHV genomeInfectious progeny virionsORF45