2024
Endothelial TGF-β Signaling Regulates Endothelial-Mesenchymal Transition During Arteriovenous Fistula Remodeling in Mice With Chronic Kidney Disease.
Zhang W, Gonzalez L, Li X, Bai H, Li Z, Taniguchi R, Langford J, Ohashi Y, Thaxton C, Yukihiko A, Yatsula B, Martin K, Goodwin J, Tellides G, Long X, Shu C, Dardik A. Endothelial TGF-β Signaling Regulates Endothelial-Mesenchymal Transition During Arteriovenous Fistula Remodeling in Mice With Chronic Kidney Disease. Arteriosclerosis Thrombosis And Vascular Biology 2024 PMID: 39297205, DOI: 10.1161/atvbaha.124.320933.Peer-Reviewed Original ResearchChronic kidney diseaseTGF-b signalingArteriovenous fistula patencyArteriovenous fistulaKidney diseaseAVF patencyEndothelial cellsAssociated with endothelial injuryArteriovenous fistula diameterImprove AVF patencyIncreased outward remodelingReduced patencyInduced endothelial-to-mesenchymal transitionAortocaval fistula modelSmooth muscle cell proliferationArteriovenous fistula failureAttenuated EndMTEnd-stage kidney diseaseHuman arteriovenous fistulaeSmooth muscle cellsMuscle cell proliferationEndothelial-to-mesenchymal transitionMouse endothelial cellsInhibition of EndMTEndothelial-mesenchymal transitionEarly thrombus formation is required of eccentric and heterogenous neointimal hyperplasia under disturbed flow
Bai H, Li Z, Zhang W, Thaxton C, Ohashi Y, Gonzalez L, Kano M, Yatsula B, Hwa J, Dardik A. Early thrombus formation is required of eccentric and heterogenous neointimal hyperplasia under disturbed flow. Journal Of Thrombosis And Haemostasis 2024 PMID: 39173878, DOI: 10.1016/j.jtha.2024.07.028.Peer-Reviewed Original ResearchEndothelial cell lossArteriovenous fistulaSmooth muscle cellsNeointimal hyperplasiaHeterogeneous neointimaCell lossThrombus formationMT/mG reporter miceHuman arteriovenous fistulaeInhibit neointimal hyperplasiaEarly thrombus formationAntiplatelet therapyC57BL/6 miceReporter miceAVF failureWistar ratsVenous systemJuxta-anastomotic areaMuscle cellsAnimal modelsVenous outflowEarly thrombusEndothelial cellsArterial inflowThrombusThe predictive role of the platelet-to-lymphocyte ratio for the risk of non-alcoholic fatty liver disease and cirrhosis: a nationwide cross-sectional study
Yan C, Zhang W, Xiao Y, Sun Y, Peng X, Cai W. The predictive role of the platelet-to-lymphocyte ratio for the risk of non-alcoholic fatty liver disease and cirrhosis: a nationwide cross-sectional study. Frontiers In Endocrinology 2024, 15: 1376894. PMID: 39040676, PMCID: PMC11260703, DOI: 10.3389/fendo.2024.1376894.Peer-Reviewed Original ResearchPlatelet-to-lymphocyte ratioNon-alcoholic fatty liver diseaseNAFLD populationRestricted cubic splinesFatty liver diseasePatients progress to cirrhosisNondiabetic populationLiver diseaseDiabetic populationNational Health and Nutrition Examination SurveyRisk of non-alcoholic fatty liver diseaseAssociated with non-alcoholic fatty liver diseaseHealth and Nutrition Examination SurveyAssociated with cirrhosisTwo-piecewise linear regressionProgression to cirrhosisNutrition Examination SurveyLogistic regression analysisNationwide cross-sectional studyMonitoring disease progressionCross-sectional studyNAFLD incidenceDisease progressionCirrhosisExamination SurveyHeterogeneous gene expression during early arteriovenous fistula remodeling suggests that downregulation of metabolism predicts adaptive venous remodeling
Ohashi Y, Protack C, Aoyagi Y, Gonzalez L, Thaxton C, Zhang W, Kano M, Bai H, Yatsula B, Alves R, Hoshina K, Schneider E, Long X, Perry R, Dardik A. Heterogeneous gene expression during early arteriovenous fistula remodeling suggests that downregulation of metabolism predicts adaptive venous remodeling. Scientific Reports 2024, 14: 13287. PMID: 38858395, PMCID: PMC11164895, DOI: 10.1038/s41598-024-64075-8.Peer-Reviewed Original ResearchConceptsGene expression patternsArteriovenous fistula groupArteriovenous fistulaExpression patternsHeterogeneous gene expressionGene expression changesOutcomes of arteriovenous fistulaVenous remodelingArteriovenous fistula maturationPostoperative day 7Reduce patient morbidityRNA sequencingUpregulation of metabolismBioinformatics analysisGene expressionDownregulation of metabolismMetabolic pathwaysExpression changesGenesAVF maturationAVF remodelingFistula creationC57BL/6 miceClinical outcomesPatient morbidityAbstract 3092: Smooth Muscle Cell Tgf-β Receptor Knockout Improves Arteriovenous Fistula Maturation Via Attenuation Of Autophagy
Zhang W, Bai H, Gonzalez L, Li Z, Ohashi Y, Thaxton C, aoyagi Y, Yatsula B, Kano M, Xie Y, Cai Y, Chang S, Dardik A. Abstract 3092: Smooth Muscle Cell Tgf-β Receptor Knockout Improves Arteriovenous Fistula Maturation Via Attenuation Of Autophagy. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: a3092-a3092. DOI: 10.1161/atvb.44.suppl_1.3092.Peer-Reviewed Original ResearchChronic kidney diseaseSmooth muscle cellsEnd-stage renal diseaseArteriovenous fistulaHuman arteriovenous fistulaeControl miceC57BL/6J miceCKD showRate of primary failureBasilic vein transpositionTGF-bModel of arteriovenous fistulaTGF-b1 expressionArteriovenous fistula failureArteriovenous fistula maturationWild type miceControl C57BL/6J miceIncreased markers of autophagyTGF-b signalingVein transpositionAortocaval fistulaAVF maturationReceptor knockoutMT/mG miceSham nephrectomyAbstract 1130: Tenascin-C In Arteriovenous Fistula Failure: Unraveling Venous Remodeling Dynamics
Gonzalez L, Zhang W, Ohashi Y, Vazquez-Padron R, Dardik A. Abstract 1130: Tenascin-C In Arteriovenous Fistula Failure: Unraveling Venous Remodeling Dynamics. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: a1130-a1130. DOI: 10.1161/atvb.44.suppl_1.1130.Peer-Reviewed Original ResearchHuman dermal microvascular endothelial cellsInferior vena cavaThrombomodulin expressionTNC expressionPost-AVF creationWild-type C57BL/6 miceMale wild-type C57BL/6 miceOne-year patency rateDecreased thrombomodulin expressionIncreased thrombomodulin expressionArteriovenous fistula failureTenascin-CSites of tissue injuryTranslational therapeutic strategiesDermal microvascular endothelial cellsNF-kB expressionMicrovascular endothelial cellsAVF patencyVena cavaAVF creationSham surgeryC57BL/6 miceFistula failureAVF failureSuperior patencyDisturbed flow in the juxta-anastomotic area of an arteriovenous fistula correlates with endothelial loss, acute thrombus formation, and neointimal hyperplasia
Bai H, Varsanik M, Thaxton C, Ohashi Y, Gonzalez L, Zhang W, Aoyagi Y, Kano M, Yatsula B, Li Z, Pocivavsek L, Dardik A. Disturbed flow in the juxta-anastomotic area of an arteriovenous fistula correlates with endothelial loss, acute thrombus formation, and neointimal hyperplasia. AJP Heart And Circulatory Physiology 2024, 326: h1446-h1461. PMID: 38578237, PMCID: PMC11380968, DOI: 10.1152/ajpheart.00054.2024.Peer-Reviewed Original ResearchConceptsEndothelial cell lossOutflow veinChronic kidney diseaseJuxta-anastomotic areaMouse AVF modelNeointimal hyperplasiaThrombus formationAcute thrombus formationCell lossHuman AVF maturationGene OntologyAVF maturationAVF patencyEarly thrombus formationArteriovenous fistulaC57BL/6 miceClinical failureWistar ratsEndothelial lossKidney diseaseImmune responseEndothelial cellsHyperplasiaMiceAVFA central arteriovenous fistula reduces systemic hypertension in a mouse model
Brahmandam A, Alves R, Liu H, Gonzalez L, Aoyagi Y, Ohashi Y, Langford J, Thaxton C, Taniguchi R, Zhang W, Bai H, Yatsula B, Dardik A. A central arteriovenous fistula reduces systemic hypertension in a mouse model. JVS Vascular Science 2024, 5: 100191. PMID: 38510938, PMCID: PMC10951512, DOI: 10.1016/j.jvssci.2024.100191.Peer-Reviewed Original ResearchCentral arteriovenous fistulaArteriovenous fistulaBlood pressureAng IISystemic hypertensionVenous remodelingArteriovenous fistula creationPCNA-positive cellsPostoperative day 7Systemic blood pressureSystolic blood pressureCollagen I depositionNormotensive miceRefractory hypertensionHypertensive miceC57BL6/J miceHypertensive environmentTreated patientsAngiotensin IIMouse modelDay 7HypertensionMiceFistulaAng
2023
Empagliflozin inhibits neointimal hyperplasia through attenuating endothelial-to-mesenchymal transition via TAK-1/NF-κB pathway
Zhang W, Li X, Li M, He H, Yang C, Wang M, Liu D, Zhang L, Shu C. Empagliflozin inhibits neointimal hyperplasia through attenuating endothelial-to-mesenchymal transition via TAK-1/NF-κB pathway. European Journal Of Pharmacology 2023, 954: 175826. PMID: 37321472, DOI: 10.1016/j.ejphar.2023.175826.Peer-Reviewed Original ResearchConceptsNeointimal hyperplasiaEmpagliflozin treatmentTreatment groupsControl groupExpression levelsFSP-1NF-κB expression levelsTAK-1Western blottingCarotid ligation injuryInflammation-induced EndMTKi-67-positive cellsP-NF-κBMale C57BL/6J miceInhibits Neointimal HyperplasiaVascular neointimal hyperplasiaInflammatory gene mRNA expressionNF-κB pathwayNF-κB signalingMRNA expression levelsGene mRNA expressionVehicle treatmentArtery ligationLigation injuryInflammatory cellsSulfasalazine induces autophagy inhibiting neointimal hyperplasia following carotid artery injuries in mice
Zhang W, Yan C, Xiao Y, Sun Y, Lin Y, Li Q, Cai W. Sulfasalazine induces autophagy inhibiting neointimal hyperplasia following carotid artery injuries in mice. Frontiers In Bioengineering And Biotechnology 2023, 11: 1199785. PMID: 37288359, PMCID: PMC10242098, DOI: 10.3389/fbioe.2023.1199785.Peer-Reviewed Original ResearchVascular smooth muscle cellsNeointimal hyperplasiaTreatment groupsSmooth muscle cellsLigation injurySSZ groupWestern blottingControl groupMuscle cellsExpression levelsVascular smooth muscle cell proliferationLC3-IILC3-II expression levelsCarotid ligation injurySmooth muscle cell proliferationCarotid artery injuryCell nuclear antigen expressionPrevention of restenosisRatio of intimaCell proliferationMuscle cell proliferationP62 protein expressionNuclear antigen expressionPhosphorylation of mTORArtery injuryEphB4 monomer inhibits chronic graft vasculopathy in an aortic transplant model
Langford J, Gonzalez L, Taniguchi R, Brahmandam A, Zhang W, Dardik A. EphB4 monomer inhibits chronic graft vasculopathy in an aortic transplant model. JVS Vascular Science 2023, 4: 100109. PMID: 37519335, PMCID: PMC10372308, DOI: 10.1016/j.jvssci.2023.100109.Peer-Reviewed Original ResearchAortic transplant modelAllograft vasculopathyT cellsTransplant modelEphrin-B2Immune cell accumulationChronic graftCardiac transplantChronic rejectionTransplanted graftAortic allograftsNeointima formationCell accumulationTissue damageMonocyte adhesionLess neointima formationVasculopathyAdherent monocytesEndothelial surfaceReceptor EphB4MacrophagesGraftMonocytesImportant targetCellsSex hormones impact early maturation and immune response in the arteriovenous fistula mouse model
Satam K, Ohashi Y, Thaxton C, Gonzalez L, Setia O, Bai H, Aoyagi Y, Xie Y, Zhang W, Yatsula B, Martin K, Cai Y, Dardik A. Sex hormones impact early maturation and immune response in the arteriovenous fistula mouse model. AJP Heart And Circulatory Physiology 2023, 325: h77-h88. PMID: 37145957, PMCID: PMC10243550, DOI: 10.1152/ajpheart.00049.2023.Peer-Reviewed Original ResearchConceptsIntact female miceAVF maturationSex hormonesT cellsFemale miceArteriovenous fistulaMale miceMouse modelHigher IL-10Arteriovenous fistula creationImmune cell recruitmentSex-specific therapiesHormone receptor signalingSex differencesHuman AVF maturationAVF surgeryMale patientsClinical outcomesFemale patientsFistula maturationIL-10C57BL/6 miceInferior outcomesVenous adaptationFistula creationEndothelial Glucocorticoid Receptor Deficiency Affects Arteriovenous Fistula Maturation
Zhang W, Gonzalez L, Thaxton C, Ohashi Y, Aoyagi Y, Xie Y, Cai Y, Shu C, Goodwin J, Dardik A. Endothelial Glucocorticoid Receptor Deficiency Affects Arteriovenous Fistula Maturation. JVS Vascular Science 2023, 4: 100147. DOI: 10.1016/j.jvssci.2023.100147.Peer-Reviewed Original Research
2022
First-in-Human Implantation of Gutter-Free Design Stent-Graft in in situ Fenestration TEVAR for Aortic Arch Pathology
Li X, Shu C, Wang L, Li Q, Fang K, Luo M, Zhang W, Zhou Y, Zhou H. First-in-Human Implantation of Gutter-Free Design Stent-Graft in in situ Fenestration TEVAR for Aortic Arch Pathology. Frontiers In Cardiovascular Medicine 2022, 9: 911689. PMID: 35845060, PMCID: PMC9279855, DOI: 10.3389/fcvm.2022.911689.Peer-Reviewed Original ResearchThoracic endovascular aortic repairAortic stent graftStent graftPolytetrafluoroethylene coatingSkirt structurePrevention of endoleaksLeft subclavian arteryAortic arch aneurysmAortic arch pathologiesEndovascular aortic repairFuture multi-centre studiesMulti-center studyFabric structureStent graft fenestrationLocalized dissectionArch aneurysmArch pathologiesAortic repairSubclavian arteryLong-term evaluationGraft fenestrationGraftHuman implantationEndoleakEndograftSex differences in arterial identity correlate with neointimal hyperplasia after balloon injury
Gao M, Gao X, Taniguchi R, Brahmandam A, Matsubara Y, Liu J, Liu H, Zhang W, Dardik A. Sex differences in arterial identity correlate with neointimal hyperplasia after balloon injury. Molecular Biology Reports 2022, 49: 8301-8315. PMID: 35715609, PMCID: PMC9463237, DOI: 10.1007/s11033-022-07644-2.Peer-Reviewed Original ResearchConceptsBalloon injuryFemale ratsArterial remodelingNeointimal proliferationCarotid arteryNeointimal thicknessSex differencesLeft common carotid arteryAtherosclerotic arterial diseaseSmooth muscle cell proliferationRight carotid arteryCarotid balloon injuryMale Sprague-DawleyCommon carotid arteryBaseline sex differencesMuscle cell proliferationErythropoietin-producing hepatocellular (Eph) receptorsBackgroundEndovascular treatmentArterial diseaseArterial injuryClinical outcomesSex hormonesUninjured controlsArterial identitySprague-DawleySimultaneous Endovascular Repair Is Not Associated With Increased Risk for Thoracic and Abdominal Aortic Pathologies: Early and Midterm Outcomes
Zhang W, Zhang L, Li X, Li M, Qiu J, Wang M, Shu C. Simultaneous Endovascular Repair Is Not Associated With Increased Risk for Thoracic and Abdominal Aortic Pathologies: Early and Midterm Outcomes. Frontiers In Cardiovascular Medicine 2022, 9: 883708. PMID: 35711338, PMCID: PMC9197242, DOI: 10.3389/fcvm.2022.883708.Peer-Reviewed Original ResearchThoracic endovascular repairEndovascular aneurysm repairAortic pathologyEndovascular repairMean survival rateSurvival rateSimultaneous endovascular repairAbdominal aortic diseaseAbdominal aortic pathologyMaximal aortic diameterConcomitant thoracicHospital stayMidterm outcomesPostoperative complicationsAortic repairPostoperative outcomesRepair patientsAneurysm repairSingle centerAortic diameterAortic diseaseMinimal morbidityAortic segmentsEffective treatmentProcedure timeEndothelial Cell TGF-β (Transforming Growth Factor-Beta) Signaling Regulates Venous Adaptive Remodeling to Improve Arteriovenous Fistula Patency
Taniguchi R, Ohashi Y, Lee JS, Hu H, Gonzalez L, Zhang W, Langford J, Matsubara Y, Yatsula B, Tellides G, Fahmy TM, Hoshina K, Dardik A. Endothelial Cell TGF-β (Transforming Growth Factor-Beta) Signaling Regulates Venous Adaptive Remodeling to Improve Arteriovenous Fistula Patency. Arteriosclerosis Thrombosis And Vascular Biology 2022, 42: 868-883. PMID: 35510552, PMCID: PMC9233042, DOI: 10.1161/atvbaha.122.317676.Peer-Reviewed Original ResearchConceptsArteriovenous fistulaSMC proliferationAVF patencyCollagen densityMouse aortocaval fistula modelTGF-β receptor IArteriovenous fistula patencyAortocaval fistula modelInhibition of TGFPredetermined time pointsTGF-β inhibitionTGF-β signalingTGF-β receptorDisruption of TGFFistula patencyAVF failureWall thicknessVascular accessVenous remodelingSuccessful hemodialysisDoppler ultrasoundFistula modelReceptor IPatencyTGFLong-term outcome of spontaneous isolated superior mesenteric artery dissection in different angiographic types
Liu D, Shu C, Li M, Li X, Yang C, Zhang W. Long-term outcome of spontaneous isolated superior mesenteric artery dissection in different angiographic types. Vascular 2022, 31: 668-677. PMID: 35344454, DOI: 10.1177/17085381221079992.Peer-Reviewed Original ResearchConceptsMesenteric artery dissectionSISMAD patientsAngiographic typeFollow-upArtery dissectionOptimal therapeutic optionType III patientsType I patientsEmergency surgical interventionPeriodic follow-upLong-term outcomesType II patientsType II groupIII patientsI patientsMedian ageII patientsRadiological reviewRetrospective studyTherapeutic optionsSurgical interventionSmoking historyClinical dataSISMADII group
2021
Roles of MicroRNAs in Peripheral Artery In‐Stent Restenosis after Endovascular Treatment
Wang M, Zhang W, Zhang L, Wang L, Li J, Shu C, Li X. Roles of MicroRNAs in Peripheral Artery In‐Stent Restenosis after Endovascular Treatment. BioMed Research International 2021, 2021: 9935671. PMID: 34368362, PMCID: PMC8337102, DOI: 10.1155/2021/9935671.Peer-Reviewed Original ResearchConceptsPercutaneous transluminal angioplastyEndovascular repairStent restenosisPeripheral arterial diseaseNovel therapeutic targetPotential therapeutic strategyMajor pathological processesTransluminal angioplastyArterial diseaseEndovascular treatmentLimited complicationsPeripheral arteriesStent implantationEndothelial denudationInflammatory responseClinical studiesNeointimal hyperplasiaTherapeutic strategiesRole of microRNAsTherapeutic targetRestenosisPathological processesLong-term successRole of miRNAsUnderlying mechanism
2020
Diagnosis and outcomes of surgical treatment of carotid bifurcation tumors
Li X, Zhang W, Shu C, Li Q, Zhang L, Zhu J. Diagnosis and outcomes of surgical treatment of carotid bifurcation tumors. Journal Of International Medical Research 2020, 48: 0300060520976495. PMID: 33317387, PMCID: PMC7739102, DOI: 10.1177/0300060520976495.Peer-Reviewed Original ResearchConceptsOperative timeBifurcation tumorsBlood lossClinicopathological featuresShamblin type ICarotid body tumorLymph node metastasisMean tumor volumePatients' clinicopathological featuresSpearman correlation analysisMalignant CBTsPostoperative complicationsComplication gradeConservative treatmentSurgical complicationsNode metastasisSurgical treatmentBody tumorsExamination findingsPathological examinationSurgical managementRetrospective studySurgical difficultyTumor sizeFrequent lesions