1998
Signal Sequence Recognition in Posttranslational Protein Transport across the Yeast ER Membrane
Plath K, Mothes W, Wilkinson B, Stirling C, Rapoport T. Signal Sequence Recognition in Posttranslational Protein Transport across the Yeast ER Membrane. Cell 1998, 94: 795-807. PMID: 9753326, DOI: 10.1016/s0092-8674(00)81738-9.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportCross-Linking ReagentsEndoplasmic ReticulumFungal ProteinsHSP70 Heat-Shock ProteinsLysineMembrane ProteinsMembrane Transport ProteinsMutagenesisPhenylalaninePhotochemistryProtein Processing, Post-TranslationalProtein Sorting SignalsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSEC Translocation ChannelsConceptsPosttranslational protein transportSignal sequence recognitionSignal sequenceProtein transportYeast ER membraneProtein-conducting channelYeast endoplasmic reticulumSequence recognitionTransmembrane domain 2Cross-linking studiesATP-independent reactionER membraneCotranslational translocationPolypeptide transportProtein channelsEndoplasmic reticulumDomain 2Membrane componentsSec61pLipid bilayersGeneral mechanismSequenceSec62pKar2pMammals
1994
Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane.
Mothes W, Prehn S, Rapoport T. Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane. The EMBO Journal 1994, 13: 3973-3982. PMID: 8076593, PMCID: PMC395317, DOI: 10.1002/j.1460-2075.1994.tb06713.x.Peer-Reviewed Original ResearchMeSH KeywordsAffinity LabelsAmino Acid SequenceAzirinesBenzoatesBiological TransportCell CompartmentationCross-Linking ReagentsDNA Mutational AnalysisEndoplasmic ReticulumLysineMembrane ProteinsModels, BiologicalMolecular Sequence DataProlactinProtein BiosynthesisProtein PrecursorsSEC Translocation ChannelsStructure-Activity RelationshipUltraviolet RaysConceptsSec61 alphaNascent chainsProtein interactsSignal sequenceProtein-conducting channelSecretory protein preprolactinNascent polypeptide chainsEndoplasmic reticulum membranePhoto-crosslinking approachSec61p complexER membraneMembrane proteinsMembrane environmentSecretory proteinsPolypeptide segmentsReticulum membranePolypeptide chainTranslocation processHydrophobic coreRibosomesProtein environmentProteinTranslocationPhotoreactive groupSequence