Self-complementary AAV Virus (scAAV) Safe and Long-term Gene Transfer in the Trabecular Meshwork of Living Rats and Monkeys
Buie L, Rasmussen C, Porterfield E, Ramgolam V, Choi V, Markovic-Plese S, Samulski R, Kaufman P, Borrás T. Self-complementary AAV Virus (scAAV) Safe and Long-term Gene Transfer in the Trabecular Meshwork of Living Rats and Monkeys. Investigative Ophthalmology & Visual Science 2009, 51: 236-248. PMID: 19684004, PMCID: PMC2869048, DOI: 10.1167/iovs.09-3847.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDependovirusEnzyme-Linked Immunosorbent AssayFemaleFluorescein AngiographyFluorescent Antibody Technique, IndirectGene ExpressionGene Transfer TechniquesGenetic VectorsGonioscopyGreen Fluorescent ProteinsIntraocular PressureMacaca fascicularisMaleOligonucleotide Array Sequence AnalysisRatsRats, Inbred BNRats, WistarReverse Transcriptase Polymerase Chain ReactionTonometry, OcularTrabecular MeshworkTransduction, GeneticConceptsHuman trabecular meshwork cellsTrabecular meshworkIntraocular pressureImmune responseCell-mediated immune responsesAnterior segment tissuesLow immune responseGene therapyTrabecular meshwork cellsSingle doseSingle dosesAAV vectorsCynomolgus monkeysFaster onsetGoldmann tonometerTransgene expressionLong-term gene transferRatsSegment tissuesProliferation assaysAdverse effectsMeshwork cellsSafe deliveryLiving ratsMonkeys