2009
Self-complementary AAV Virus (scAAV) Safe and Long-term Gene Transfer in the Trabecular Meshwork of Living Rats and Monkeys
Buie L, Rasmussen C, Porterfield E, Ramgolam V, Choi V, Markovic-Plese S, Samulski R, Kaufman P, Borrás T. Self-complementary AAV Virus (scAAV) Safe and Long-term Gene Transfer in the Trabecular Meshwork of Living Rats and Monkeys. Investigative Ophthalmology & Visual Science 2009, 51: 236-248. PMID: 19684004, PMCID: PMC2869048, DOI: 10.1167/iovs.09-3847.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDependovirusEnzyme-Linked Immunosorbent AssayFemaleFluorescein AngiographyFluorescent Antibody Technique, IndirectGene ExpressionGene Transfer TechniquesGenetic VectorsGonioscopyGreen Fluorescent ProteinsIntraocular PressureMacaca fascicularisMaleOligonucleotide Array Sequence AnalysisRatsRats, Inbred BNRats, WistarReverse Transcriptase Polymerase Chain ReactionTonometry, OcularTrabecular MeshworkTransduction, GeneticConceptsHuman trabecular meshwork cellsTrabecular meshworkIntraocular pressureImmune responseCell-mediated immune responsesAnterior segment tissuesLow immune responseGene therapyTrabecular meshwork cellsSingle doseSingle dosesAAV vectorsCynomolgus monkeysFaster onsetGoldmann tonometerTransgene expressionLong-term gene transferRatsSegment tissuesProliferation assaysAdverse effectsMeshwork cellsSafe deliveryLiving ratsMonkeys
2008
Simvastatin Inhibits IL-17 Secretion by Targeting Multiple IL-17-Regulatory Cytokines and by Inhibiting the Expression of IL-17 Transcription Factor RORC in CD4+ Lymphocytes
Zhang X, Jin J, Peng X, Ramgolam V, Markovic-Plese S. Simvastatin Inhibits IL-17 Secretion by Targeting Multiple IL-17-Regulatory Cytokines and by Inhibiting the Expression of IL-17 Transcription Factor RORC in CD4+ Lymphocytes. The Journal Of Immunology 2008, 180: 6988-6996. PMID: 18453621, DOI: 10.4049/jimmunol.180.10.6988.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCD4-Positive T-LymphocytesCytokinesEnzyme-Linked Immunosorbent AssayFemaleFlow CytometryGene ExpressionHumansImmunologic FactorsInterleukin-17MaleMonocytesMultiple SclerosisNuclear ProteinsOligonucleotide Array Sequence AnalysisReceptors, Cytoplasmic and NuclearReverse Transcriptase Polymerase Chain ReactionSimvastatinSuppressor of Cytokine Signaling 3 ProteinSuppressor of Cytokine Signaling ProteinsT-Lymphocyte SubsetsConceptsAutoimmune responseMultiple sclerosisImmunomodulatory mechanismsT cellsIL-23 gene expressionTranscription factor RORCIL-17 productionIL-17 secretionIL-27 productionChronic inflammatory diseaseNovel immunomodulatory mechanismGood safety profileIL-17 transcriptionPromising therapeutic approachCholesterol-lowering agentsHuman autoimmune responseImmunomodulatory agentsSafety profileAutoimmune diseasesIL-6IL-4Inflammatory diseasesSpecific effectsTherapeutic approachesIFN-gamma