2013
Enhancers and Super Enhancers Are Associated With Genes That Control Phenotypic Traits In Primary Human Erythroid Cells
Schulz V, Lezon-Geyda K, Maksimova Y, Gallagher P. Enhancers and Super Enhancers Are Associated With Genes That Control Phenotypic Traits In Primary Human Erythroid Cells. Blood 2013, 122: 1200. DOI: 10.1182/blood.v122.21.1200.1200.Peer-Reviewed Original ResearchTranscriptional start siteRegulation of programsSuper enhancersErythroid cellsGene expressionErythroid enhancerTF motifsTranscription factorsHistone H3 lysine 4 monomethylationSpecific enhancersH3K27ac modificationGWAS catalogGene locusHistone H3 lysine 27 acetylationCell type-specific enhancersPrimary human erythroid cellsH3 lysine 27 acetylationAlpha-globin gene clusterDisease-associated genetic variantsGenome-wide mapsNHGRI GWAS catalogSubset of enhancersHundreds of kilobasesLysine 27 acetylationSpecialized cell types
2012
Identification of Biologicaly Relevant Enhancers in Human Erythroid Cells
Su M, Steiner L, Bogardus H, Schulz V, Hardison R, Gallagher P. Identification of Biologicaly Relevant Enhancers in Human Erythroid Cells. Blood 2012, 120: 368. DOI: 10.1182/blood.v120.21.368.368.Peer-Reviewed Original ResearchTranscriptional start siteHuman erythroid cellsCell type-specific enhancersErythroid transcription factorsErythroid-specific genesTranscription factorsErythroid cellsCandidate enhancersSpecific enhancersSignificant enrichmentGene expressionPrimary human erythroid cellsRegulation of programsDisease-associated genetic variantsGenome-wide mapsHundreds of kilobasesSpecialized cell typesTranscription factor sitesKb of genesGene expression analysisParallel gene expression analysisGenomic vicinityGenomic methodologiesEvolutionary analysisHigh conservation
2011
Unbiased Identification of Functional Barrier Insulators in Primary Human Erythroid Cells,
Steiner L, Schulz V, Maksimova Y, Seidel N, Bodine D, Gallagher P. Unbiased Identification of Functional Barrier Insulators in Primary Human Erythroid Cells,. Blood 2011, 118: 3385. DOI: 10.1182/blood.v118.21.3385.3385.Peer-Reviewed Original ResearchHuman erythroid cellsPrimary human erythroid cellsPosition-effect variegationInsulator functionErythroid cellsCpG methylationBarrier insulatorsGene promoterCell type-specific gene expressionRepressive histone mark H3K27me3Unbiased identificationParallel sequencingUpstream stimulatory factor proteinsCTCF ChIP-seqMethyl-Binding DomainGenome-wide bindingGenome-wide mapsRole of CTCFCell type-specific mannerGenome-wide scaleHistone mark H3K27me3Chicken erythroid cellsDirection of transcriptionMultiple regulatory proteinsChIP-seq studiesIntegrated Genome-Wide CTCF and CohesinSA1 Occupancy and Expression Analyses in Erythropoiesis
Schulz V, Steiner L, Maksimova Y, Gallagher P. Integrated Genome-Wide CTCF and CohesinSA1 Occupancy and Expression Analyses in Erythropoiesis. Blood 2011, 118: 1305. DOI: 10.1182/blood.v118.21.1305.1305.Peer-Reviewed Original ResearchSites of CTCFCTCF sitesCell-type specificIntergenic regionErythroid cellsChromatin domainsCohesin complexCell typesGene promoterRefSeq genesLong-range chromatin interactionsPrimary human erythroid cellsRepressive chromatin marksCell type-specific mannerDistal intergenic regionsMacromolecule catabolic processHigh-throughput sequencingHuman erythroid cellsMRNA transcriptome analysisChromatin marksChromatin interactionsChromosome segregationInduction of apoptosisCTCF bindingTranscriptional activator