2018
Fast, in vivo voltage imaging using a red fluorescent indicator
Kannan M, Vasan G, Huang C, Haziza S, Li JZ, Inan H, Schnitzer MJ, Pieribone VA. Fast, in vivo voltage imaging using a red fluorescent indicator. Nature Methods 2018, 15: 1108-1116. PMID: 30420685, PMCID: PMC6516062, DOI: 10.1038/s41592-018-0188-7.Peer-Reviewed Original ResearchConceptsOptical toolsOptical toolboxUnparalleled temporal resolutionRed fluorescent indicatorVoltage imagingOptical electrophysiologyModest illumination intensitiesHigh-throughput strategyVoltage indicatorsIllumination intensityNew hueAcute brain slicesMultispectral imagingGreen probesSubthreshold voltageTemporal resolutionEnhanced sensitivityPostsynaptic potentialsMRuby3Brain slicesHippocampal rhythmsActivity monitorFunctional imagingImagingGEVIsA Dexterous, Glove-Based Teleoperable Low-Power Soft Robotic Arm for Delicate Deep-Sea Biological Exploration
Phillips BT, Becker KP, Kurumaya S, Galloway KC, Whittredge G, Vogt DM, Teeple CB, Rosen MH, Pieribone VA, Gruber DF, Wood RJ. A Dexterous, Glove-Based Teleoperable Low-Power Soft Robotic Arm for Delicate Deep-Sea Biological Exploration. Scientific Reports 2018, 8: 14779. PMID: 30283051, PMCID: PMC6170437, DOI: 10.1038/s41598-018-33138-y.Peer-Reviewed Original ResearchConceptsMarine biologistsDeep-sea organismsMultitude of organismsCoral reefsMarine organismsMarine systemsShallow marine systemsBiological interactionsBiological explorationOrganismsDeep seaSuction samplerBiologistsBiological samplingScuba divingDelicate interactionSCUBABiodiversityField trialsFaunaReefsEcosystemsInteractionPhysiologyClawsGenetically encoded fluorescent voltage indicators: are we there yet?
Platisa J, Pieribone VA. Genetically encoded fluorescent voltage indicators: are we there yet? Current Opinion In Neurobiology 2018, 50: 146-153. PMID: 29501950, PMCID: PMC5984684, DOI: 10.1016/j.conb.2018.02.006.Peer-Reviewed Original Research
2017
Optimizing recruitment and retention of adolescents in ED research: Findings from concussion biomarker pilot study
Mbachu SN, Pieribone VA, Bechtel KA, McCarthy ML, Melnick ER. Optimizing recruitment and retention of adolescents in ED research: Findings from concussion biomarker pilot study. The American Journal Of Emergency Medicine 2017, 36: 884-887. PMID: 28918968, DOI: 10.1016/j.ajem.2017.09.014.Peer-Reviewed Original ResearchDirected Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexElectric StimulationEmbryo, MammalianGreen Fluorescent ProteinsHEK293 CellsHumansLuminescent ProteinsMiceModels, MolecularMolecular BiologyMutagenesis, Site-DirectedMutationNeuronsPatch-Clamp TechniquesRecombinant Fusion ProteinsTransfectionVoltage-Sensitive Dye Imaging
2016
Dickkopf-related Protein 3 as a Sensitive and Specific Marker for Cerebrospinal Fluid Leaks
Michaelides EM, Kuang H, Pieribone VA. Dickkopf-related Protein 3 as a Sensitive and Specific Marker for Cerebrospinal Fluid Leaks. Otology & Neurotology 2016, 37: 299-303. PMID: 26837002, DOI: 10.1097/mao.0000000000000954.Peer-Reviewed Original ResearchA Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices
Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. Journal Of Neuroscience 2016, 36: 2458-2472. PMID: 26911693, PMCID: PMC4764664, DOI: 10.1523/jneurosci.3484-15.2016.Peer-Reviewed Original ResearchConceptsVoltage indicatorsBlue-shifted channelrhodopsinRed-shifted fluorescent proteinsFluorescent voltage indicatorsWide-field fluorescence microscopyBlue excitationOptical imagingOptical electrophysiologyLow phototoxicityAutofluorescent backgroundLight photoactivationSingle-trial recordingsTemporal resolutionChannelrhodopsinIntrinsic advantagesExcitationVoltage oscillationsFluorescence microscopyOscillationsGreen indicatorsChromophoreMicroscopyResolution
2015
Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment
Gruber DF, Gaffney JP, Mehr S, DeSalle R, Sparks JS, Platisa J, Pieribone VA. Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment. PLOS ONE 2015, 10: e0140972. PMID: 26561348, PMCID: PMC4641735, DOI: 10.1371/journal.pone.0140972.Peer-Reviewed Original ResearchConceptsAcid-binding proteinFluorescent proteinFatty acid-binding proteinDuplication eventsGene duplication eventsStrong positive selectionNew fluorescent proteinsMarine environmentAdaptive evolutionEvolutionary switchCryptic genusVertebrate ordersVertebrate taxaPhylogenetic reconstructionProtein familyPhylogenetic analysisSequence motifsSister groupEvolutionary adaptationTranscriptomic analysisPositive selectionFatty acid binding proteinAcid binding proteinBinding proteinBright green fluorescence
2014
Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight
Han Z, Jin L, Chen F, Loturco JJ, Cohen LB, Bondar A, Lazar J, Pieribone VA. Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight. PLOS ONE 2014, 9: e113873. PMID: 25419571, PMCID: PMC4242678, DOI: 10.1371/journal.pone.0113873.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAmino AcidsAnimalsCells, CulturedFluorescenceFluorescent DyesGreen Fluorescent ProteinsHEK293 CellsHumansHydrogen-Ion ConcentrationKineticsLuminescent ProteinsMembrane PotentialsMicroscopy, ConfocalMutation, MissenseNeuronsPatch-Clamp TechniquesPrenylationRatsRecombinant Fusion ProteinsSpectrometry, Fluorescence
2013
Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics
Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA. Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics. PLOS ONE 2013, 8: e81295. PMID: 24312287, PMCID: PMC3842285, DOI: 10.1371/journal.pone.0081295.Peer-Reviewed Original Research
2012
Design Constraints for Mobile, High-Speed Fluorescence Brain Imaging in Awake Animals
Osman A, Park JH, Dickensheets D, Platisa J, Culurciello E, Pieribone VA. Design Constraints for Mobile, High-Speed Fluorescence Brain Imaging in Awake Animals. IEEE Transactions On Biomedical Circuits And Systems 2012, 6: 446-453. PMID: 23853231, DOI: 10.1109/tbcas.2012.2226174.Peer-Reviewed Original ResearchA Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials
Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T. A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials. PLOS ONE 2012, 7: e43454. PMID: 22970127, PMCID: PMC3435330, DOI: 10.1371/journal.pone.0043454.Peer-Reviewed Original ResearchSingle Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe
Jin L, Han Z, Platisa J, Wooltorton JR, Cohen LB, Pieribone VA. Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe. Neuron 2012, 75: 779-785. PMID: 22958819, PMCID: PMC3439164, DOI: 10.1016/j.neuron.2012.06.040.Peer-Reviewed Original ResearchGenetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics
Baker BJ, Jin L, Han Z, Cohen LB, Popovic M, Platisa J, Pieribone V. Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics. Journal Of Neuroscience Methods 2012, 208: 190-196. PMID: 22634212, PMCID: PMC3398169, DOI: 10.1016/j.jneumeth.2012.05.016.Peer-Reviewed Original Research
2011
Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells
Jin L, Baker B, Mealer R, Cohen L, Pieribone V, Pralle A, Hughes T. Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells. Journal Of Neuroscience Methods 2011, 199: 1-9. PMID: 21497167, PMCID: PMC3281265, DOI: 10.1016/j.jneumeth.2011.03.028.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell LineCell Line, TumorCell MembraneCytosolDNA Transposable ElementsFluorescent DyesHumansKidneyLuminescent ProteinsMembrane PotentialsMembrane ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceModels, MolecularMutation, MissenseNeuroblastomaPatch-Clamp TechniquesPeptide FragmentsProtein FoldingProtein MultimerizationProtein Structure, SecondaryProtein Structure, TertiaryRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsTransfectionConceptsShaker subunitsYellow fluorescent proteinEndoplasmic reticulumMammalian cellsNon-fluorescent halvesMisfolded monomersPlasma membrane expressionFluorescent protein VenusShaker potassium channelFluorescent protein (FP) voltage sensorsMembrane localizationPlasma membraneFluorescent proteinRandom insertionMembrane expressionSubunitsMembrane potentialIntracellular fluorescencePotassium channelsCellsFluorescent probeΔF/FVoltage sensorTetramerizationProtein
2008
Genetically encoded fluorescent sensors of membrane potential
Baker BJ, Mutoh H, Dimitrov D, Akemann W, Perron A, Iwamoto Y, Jin L, Cohen LB, Isacoff EY, Pieribone VA, Hughes T, Knöpfel T. Genetically encoded fluorescent sensors of membrane potential. Brain Cell Biology 2008, 36: 53. PMID: 18679801, PMCID: PMC2775812, DOI: 10.1007/s11068-008-9026-7.Peer-Reviewed Original Research
2007
Clinical Evaluation of Ganaxolone in Pediatric and Adolescent Patients with Refractory Epilepsy
Pieribone VA, Tsai J, Soufflet C, Rey E, Shaw K, Giller E, Dulac O. Clinical Evaluation of Ganaxolone in Pediatric and Adolescent Patients with Refractory Epilepsy. Epilepsia 2007, 48: 1870-1874. PMID: 17634060, DOI: 10.1111/j.1528-1167.2007.01182.x.Peer-Reviewed Original ResearchConceptsRefractory epilepsyAdverse eventsPilot studyDose escalation scheduleExtension periodDose-escalation trialEscalation scheduleSubstantial respondersAntiepileptic therapyTreat analysisGeneralized epilepsyModerate respondersAdolescent patientsClinical evaluationGanaxoloneClinical studiesPediatric subjectsPotential efficacyAnticonvulsant activityEpilepsyAdolescent subjectsOral suspensionExtension phaseDose rangeFurther studies
2006
Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells
Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK. Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells. Journal Of Neuroscience Methods 2006, 161: 32-38. PMID: 17126911, DOI: 10.1016/j.jneumeth.2006.10.005.Peer-Reviewed Original Research
2004
A role for talin in presynaptic function
Morgan JR, Di Paolo G, Werner H, Shchedrina VA, Pypaert M, Pieribone VA, De Camilli P. A role for talin in presynaptic function. Journal Of Cell Biology 2004, 167: 43-50. PMID: 15479735, PMCID: PMC2172527, DOI: 10.1083/jcb.200406020.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBlotting, WesternCell AdhesionClathrinEndocytosisGlutathione TransferaseHumansLampreysLipid MetabolismMicroscopy, ConfocalMicroscopy, Electron, TransmissionPeptidesPhosphotransferases (Alcohol Group Acceptor)Protein BindingProtein Structure, TertiaryRatsSynapsesTalinTime FactorsConceptsSynaptic vesicle endocytosisClathrin-mediated synaptic vesicle endocytosisClathrin-coated pitsEndocytic defectsMajor phosphatidylinositolActin cytoskeletonVesicle endocytosisActin dynamicsKinase interactionTalinPIP kinasePresynaptic compartmentFunctional linkCell adhesionNeuronal synapsesEndocytosisSynaptic rolePresynaptic functionSynaptic actinActinPhosphatidylinositolSynaptic structureIgammaClathrinCytoskeleton
2000
Severe deficiencies in dopamine signaling in presymptomatic Huntington's disease mice
Bibb J, Yan Z, Svenningsson P, Snyder G, Pieribone V, Horiuchi A, Nairn A, Messer A, Greengard P. Severe deficiencies in dopamine signaling in presymptomatic Huntington's disease mice. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 6809-6814. PMID: 10829080, PMCID: PMC18747, DOI: 10.1073/pnas.120166397.Peer-Reviewed Original ResearchConceptsMedium spiny neuronsDisease miceSpiny neuronsStriatal medium spiny neuronsHuntington's diseaseSevere deficiencyHuntington's disease miceHD model miceModel miceDopaminergic neurotransmissionHD miceSelective neurodegenerationHD pathologyMiceDARPP-32DopamineHuman huntingtinBehavioral phenotypesDiseaseNeuronsIon channelsTotal levelsDisease-causing formHuntingtinDeficiency