2013
Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics
Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA. Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics. PLOS ONE 2013, 8: e81295. PMID: 24312287, PMCID: PMC3842285, DOI: 10.1371/journal.pone.0081295.Peer-Reviewed Original Research
2011
Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells
Jin L, Baker B, Mealer R, Cohen L, Pieribone V, Pralle A, Hughes T. Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells. Journal Of Neuroscience Methods 2011, 199: 1-9. PMID: 21497167, PMCID: PMC3281265, DOI: 10.1016/j.jneumeth.2011.03.028.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell LineCell Line, TumorCell MembraneCytosolDNA Transposable ElementsFluorescent DyesHumansKidneyLuminescent ProteinsMembrane PotentialsMembrane ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceModels, MolecularMutation, MissenseNeuroblastomaPatch-Clamp TechniquesPeptide FragmentsProtein FoldingProtein MultimerizationProtein Structure, SecondaryProtein Structure, TertiaryRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsTransfectionConceptsShaker subunitsYellow fluorescent proteinEndoplasmic reticulumMammalian cellsNon-fluorescent halvesMisfolded monomersPlasma membrane expressionFluorescent protein VenusShaker potassium channelFluorescent protein (FP) voltage sensorsMembrane localizationPlasma membraneFluorescent proteinRandom insertionMembrane expressionSubunitsMembrane potentialIntracellular fluorescencePotassium channelsCellsFluorescent probeΔF/FVoltage sensorTetramerizationProtein
2006
Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells
Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK. Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells. Journal Of Neuroscience Methods 2006, 161: 32-38. PMID: 17126911, DOI: 10.1016/j.jneumeth.2006.10.005.Peer-Reviewed Original Research
1999
Inhibition of neurotransmitter release in the lamprey reticulospinal synapse by antibody-mediated disruption of SNAP-25 function
Low P, Norlin T, Risinger C, Larhammar D, Pieribone V, Shupliakov O, Brodin L. Inhibition of neurotransmitter release in the lamprey reticulospinal synapse by antibody-mediated disruption of SNAP-25 function. European Journal Of Cell Biology 1999, 78: 787-793. PMID: 10604655, DOI: 10.1016/s0171-9335(99)80029-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAxonsCell MembraneImmunohistochemistryIn Situ HybridizationLampreysMembrane ProteinsMicroinjectionsMicroscopy, ElectronNerve Tissue ProteinsNeurotransmitter AgentsProtein BindingRecombinant Fusion ProteinsSpinal CordSynapsesSynaptic TransmissionSynaptic VesiclesSynaptosomal-Associated Protein 25ConceptsPlasma membraneSynaptic vesiclesSNAP-25Lamprey giant reticulospinal synapsePresynaptic plasma membraneSynaptic vesicle exocytosisLamprey reticulospinal synapseSNARE protein SNAP-25Protein SNAP-25Synaptic vesicle clustersNeurotransmitter releaseSNARE complexMolecular machineryVesicle exocytosisMembrane fusionVesicle cyclingPresynaptic microinjectionVesicle clustersSyntaxinPresynaptic membraneVesiclesElectron microscopic analysisAntibody-mediated disruptionMembraneMicroscopic analysis