2002
A protein kinase A–dependent molecular switch in synapsins regulates neurite outgrowth
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P. A protein kinase A–dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neuroscience 2002, 5: 431-437. PMID: 11976703, DOI: 10.1038/nn840.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesBucladesineCells, CulturedConserved SequenceCyclic AMP-Dependent Protein KinasesEmbryo, NonmammalianEnzyme ActivatorsEnzyme InhibitorsGenes, ReporterMicroinjectionsMolecular Sequence DataMutagenesis, Site-DirectedNeuritesNeuronsPhosphorylationRNA, MessengerSequence Homology, Amino AcidSynapsinsXenopus laevisConceptsProtein kinase ANeurite outgrowthCAMP/protein kinase APhospho-specific antibodiesSingle amino acid residueX. laevis embryosAmino acid residuesDependent molecular switchPhosphorylation of synapsinCyclic AMPEmbryonic neuronal culturesNeuronal cell lineProtein kinaseConstitutive phosphorylationKinase AMolecular approachesMolecular switchAcid residuesLaevis embryosPKA inhibitorPotential molecular approachPhosphorylationGrowth-promoting actionCell linesSynapsin
1999
Regulation of Synaptotagmin I Phosphorylation by Multiple Protein Kinases
Hilfiker S, Pieribone V, Nordstedt C, Greengard P, Czernik A. Regulation of Synaptotagmin I Phosphorylation by Multiple Protein Kinases. Journal Of Neurochemistry 1999, 73: 921-932. PMID: 10461881, DOI: 10.1046/j.1471-4159.1999.0730921.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCalcium-Binding ProteinsCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesCasein Kinase IICell DifferentiationClathrinConserved SequenceHumansIsoenzymesMembrane GlycoproteinsMolecular Sequence DataNerve Tissue ProteinsPC12 CellsPeptide MappingPhosphoamino AcidsPhosphorylationProtein Kinase CProtein KinasesProtein Serine-Threonine KinasesRatsSynaptosomesSynaptotagmin ISynaptotagminsConceptsProtein kinase CSynaptotagmin IProtein kinaseKinase IISynaptic vesicle life cycleCasein kinase IIMultiple protein kinasesClathrin-coated vesiclesDependent protein kinase IICalcium-triggered exocytosisProtein kinase IICytoplasmic domainTryptic phosphopeptidesPhosphorylation stateRat synaptotagmin IKinase CCalcium sensorPhosphorylationKinaseSame residuesSynaptic vesiclesPC12 cellsPhorbol esterThr112I phosphorylationSynapsins as regulators of neurotransmitter release
Hilfiker S, Pieribone V, Czernik A, Kao H, Augustine G, Greengard P. Synapsins as regulators of neurotransmitter release. Philosophical Transactions Of The Royal Society B Biological Sciences 1999, 354: 269-279. PMID: 10212475, PMCID: PMC1692497, DOI: 10.1098/rstb.1999.0378.Peer-Reviewed Original ResearchConceptsSynapsin functionSynaptic vesiclesSynaptic vesicle life cycleReserve poolNeuron-specific phosphoproteinNeurotransmitter releaseSelective disruptionVesicle dockingMultigene familyNumerous proteinsCytoskeletal meshworkAbundant proteinsCytoskeletal proteinsMolecular mechanismsProtein componentsSynapsinSynapse formationPresynaptic nerve terminalsVesiclesProteinSecond roleRelease of neurotransmittersLife cycleCurrent understandingSquid giant synapse