2016
O-Glycosylation of a Secretory Granule Membrane Enzyme Is Essential for Its Endocytic Trafficking*
Vishwanatha KS, Bäck N, Lam TT, Mains RE, Eipper BA. O-Glycosylation of a Secretory Granule Membrane Enzyme Is Essential for Its Endocytic Trafficking*. Journal Of Biological Chemistry 2016, 291: 9835-9850. PMID: 26961877, PMCID: PMC4850319, DOI: 10.1074/jbc.m115.711838.Peer-Reviewed Original ResearchConceptsHigh molecular weight complexesPAM-1Molecular weight complexesEndocytic traffickingCytosolic domainBlue native PAGE analysisAtT-20 corticotrope tumor cellsWeight complexesCrucial post-translational modificationPost-translational modificationsO-glycosylation sitesPeptidylglycine αFurin-like convertasesNative PAGE analysisSoluble fragmentCorticotrope tumor cellsAlternative splicingEndocytic pathwayCatalytic domainEndocytic compartmentsGlycosylation sitesO-glycosylationMultivesicular bodiesMembrane enzymeEndoproteolytic cleavage
2010
N-Glycosylation at the SynCAM (Synaptic Cell Adhesion Molecule) Immunoglobulin Interface Modulates Synaptic Adhesion*
Fogel AI, Li Y, Giza J, Wang Q, Lam TT, Modis Y, Biederer T. N-Glycosylation at the SynCAM (Synaptic Cell Adhesion Molecule) Immunoglobulin Interface Modulates Synaptic Adhesion*. Journal Of Biological Chemistry 2010, 285: 34864-34874. PMID: 20739279, PMCID: PMC2966101, DOI: 10.1074/jbc.m110.120865.Peer-Reviewed Original ResearchConceptsN-glycosylationTrans-synaptic interactionsN-glycansSite-specific N-glycansSynaptic cell adhesion molecule 1Site-specific N-glycosylationTrans-synaptic adhesionPost-translational modificationsSelect adhesion moleculesMutational studiesSynaptic adhesionGlycosylation sitesHeterophilic interactionsIg1 domainSynapse inductionPostsynaptic membraneAdhesion moleculesNeurobiological questionsSynaptic cleftStructural modelingPresynaptic terminalsDifferential mannerSialic acidCell adhesion molecule-1Adhesion