2016
Evaluation of the Nicotinic Acetylcholine Receptor-Associated Proteome at Baseline and Following Nicotine Exposure in Human and Mouse Cortex
McClure-Begley TD, Esterlis I, Stone KL, Lam TT, Grady SR, Colangelo CM, Lindstrom JM, Marks MJ, Picciotto MR. Evaluation of the Nicotinic Acetylcholine Receptor-Associated Proteome at Baseline and Following Nicotine Exposure in Human and Mouse Cortex. ENeuro 2016, 3: eneuro.0166-16.2016. PMID: 27559543, PMCID: PMC4985585, DOI: 10.1523/eneuro.0166-16.2016.Peer-Reviewed Original ResearchConceptsPutative interacting proteinsQuantitative proteomic assessmentProtein-protein interactionsTemporal cortex tissueChaperone familyMood disordersInteracting proteinProtein complexesPresynaptic neurotransmitter releaseNovel etiological mechanismsNicotine exposureProteomic assessmentProteomeNicotinic acetylcholine receptorsMouse cortexProteinHigh-affinity nAChRsΒ2 subunitCortex of miceMaintenance of smokingNew treatment targetsResult of smokingNeurotransmitter releaseAcetylcholine receptorsPrimary function
2002
Mapping of protein:protein contact surfaces by hydrogen/deuterium exchange, followed by on-line high-performance liquid chromatography–electrospray ionization fourier-transform ion-cyclotron-resonance mass analysis
Lam TT, Lanman JK, Emmett MR, Hendrickson CL, Marshall AG, Prevelige PE. Mapping of protein:protein contact surfaces by hydrogen/deuterium exchange, followed by on-line high-performance liquid chromatography–electrospray ionization fourier-transform ion-cyclotron-resonance mass analysis. Journal Of Chromatography A 2002, 982: 85-95. PMID: 12489858, DOI: 10.1016/s0021-9673(02)01357-2.Peer-Reviewed Original ResearchConceptsHigh-Performance Liquid Chromatography-Electrospray IonizationMass analysisHydrogen/deuterium exchangeLiquid Chromatography-Electrospray IonizationNuclear magnetic resonance analysisX-ray diffractionFront-end separationMagnetic resonance analysisBackbone amide hydrogensProtein contact surfacesAmide hydrogensDeuterium exchangeConventional X-ray diffractionH/2H exchangeReaction periodResonance analysisLow concentrationsDiffractionProtein complexesComplexedHydrogenComplexesIonizationSeparationSurface