2023
High dietary K+ intake inhibits proximal tubule transport
Wang T, Liu T, Xu S, Frindt G, Weinstein A, Palmer L. High dietary K+ intake inhibits proximal tubule transport. American Journal Of Physiology. Renal Physiology 2023, 325: f224-f234. PMID: 37318989, PMCID: PMC10396284, DOI: 10.1152/ajprenal.00013.2023.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFree-flow micropunctureUrine volumeFractional excretion of Na<sup>+</sup>Proximal tubulesFiltration rateFractional NaNephron segmentsExcretion of Na<sup>+</sup>Dietary K<sup>+</sup>NHE3 protein expressionExchanger isoform 3Increased urine volumeIncreased fractional excretionVolume reabsorptionAbsolute NaKidney functionProtein expressionIsoform 3PT transportAnesthetized animalsReabsorptionWestern blottingRatsExcretionHigh Dietary K Intake Inhibits Proximal Tubule Transport and Reduces GFR
Wang T, Liu T, Xu S, Frindt G, Palmer L, Weinstein A. High Dietary K Intake Inhibits Proximal Tubule Transport and Reduces GFR. Physiology 2023, 38: 5731363. DOI: 10.1152/physiol.2023.38.s1.5731363.Peer-Reviewed Original ResearchGlomerular filtration rateReduced glomerular filtration rateK + excretionProximal tubulesTubular fluidConnecting tubuleUrine volumeTreated ratsNephron segmentsReduction of glomerular filtration rateProximal tubule transportLengths of proximal tubulesDistal nephron segmentsProximal nephron segmentsFree-flow micropunctureNa + absorptionHK intakeIncreased FENaTubule transportFractional deliveryFiltration rateKidney functionIncreased secretionFluid absorptionFITC-inulin
2022
Regulation of Glomerulotubular Balance IV: Implication of Aquaporin 1 in Flow-Dependent Proximal Tubule Transport and Cell Volume
Du Z, Yan Q, Weinstein A, Wang T. Regulation of Glomerulotubular Balance IV: Implication of Aquaporin 1 in Flow-Dependent Proximal Tubule Transport and Cell Volume. Journal Of The American Society Of Nephrology 2022, 33: 142-143. DOI: 10.1681/asn.20223311s1142d.Peer-Reviewed Original ResearchRegulation of glomerulotubular balance. IV. Implication of aquaporin 1 in flow-dependent proximal tubule transport and cell volume
Du Z, Yan Q, Shen E, Weinstein A, Wang T. Regulation of glomerulotubular balance. IV. Implication of aquaporin 1 in flow-dependent proximal tubule transport and cell volume. American Journal Of Physiology. Renal Physiology 2022, 323: f642-f653. PMID: 36108052, PMCID: PMC9705020, DOI: 10.1152/ajprenal.00167.2022.Peer-Reviewed Original ResearchConceptsWater channel aquaporin-1Proximal tubulesKO miceAquaporin-1Water reabsorptionMouse PTNo significant differenceWild-typeTight junctionsRat kidneyAquaporin-1 knockoutSignificant differenceAcid-base parametersKidney proximal tubulesRenal clearance experimentsWT miceKnockout miceUrine flowFlow stimulationFluid absorptionUrine pHClearance experimentsMouse kidneyMiceRats
2021
Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice
Xu S, Li J, Yang L, Wang CJ, Liu T, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice. Pflügers Archiv - European Journal Of Physiology 2021, 473: 1749-1760. PMID: 34455480, PMCID: PMC8528772, DOI: 10.1007/s00424-021-02611-5.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFemale miceUrine volumeNaCl cotransporterLow K intakeMeasurement of functionSex differencesNatriuretic responseFractional excretionK intakeTransport protein expressionFiltration rateNCC abundanceNCC expressionRenal clearanceDistal nephronLK dietCation excretionPlasma KNCC proteinNKCC2 expressionWestern blottingProtein expressionMiceFENaRestoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease
Du Z, Tian X, Ma M, Somlo S, Weinstein AM, Wang T. Restoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease. JCI Insight 2021, 6: e146041. PMID: 33886508, PMCID: PMC8262298, DOI: 10.1172/jci.insight.146041.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateGlomerulotubular balanceRenal cyst formationCyst formationReceptor 1 antagonistPolycystic kidney diseaseKidney weightUntreated miceDA1 antagonistControl miceKidney diseaseFiltration rateFractional reabsorptionCystic indexMouse modelCyst growthConditional KOHCO3- absorptionHeterozygous miceSame antagonistsMicePT transportAntagonistEpithelial ciliaHCO3- transportDeletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse
Thomson R, Dynia DW, Burlein S, Thomson BR, Booth C, Knauf F, Wang T, Aronson P. Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse. American Journal Of Physiology. Renal Physiology 2021, 320: f1106-f1122. PMID: 33938239, PMCID: PMC8285649, DOI: 10.1152/ajprenal.00556.2020.Peer-Reviewed Original ResearchConceptsKsp-cadherinCell adhesion moleculeAtypical memberKidney developmentMammalian kidneyAdult mammalian kidneyBasolateral membraneNormal kidney developmentEpithelial cellsAdhesion moleculesMutant animalsExpression analysisSpecific expressionE-cadherin expressionWestern blot analysisEpithelial phenotypePrincipal proteinE-cadherinBlot analysisMouse linesAquaporin-2CadherinCritical roleDevelopmental delayKnockout mice
2020
Replicated risk CACNA1C variants for major psychiatric disorders may serve as potential therapeutic targets for the shared depressive endophenotype.
Guo X, Fu Y, Zhang Y, Wang T, Lu L, Luo X, Wang K, Huang J, Xie T, Zheng C, Yang K, Tong J, Zuo L, Kang L, Tan Y, Jiang K, Li CR, Luo X. Replicated risk CACNA1C variants for major psychiatric disorders may serve as potential therapeutic targets for the shared depressive endophenotype. 2020, 4 PMID: 34046650, PMCID: PMC8153461.Peer-Reviewed Original ResearchMajor psychiatric disordersGenome-wide association studiesL-type voltage-gated calcium channelsMajor depressive disorderMRNA expression regulationPsychiatric disordersTherapeutic targetBipolar disorderChronic mild stress (CMS) ratsHuman hippocampusExpression regulationDepressive endophenotypeType voltage-gated calcium channelsRole of venlafaxineVoltage-gated calcium channelsPotential therapeutic targetRisk genesSignificant mRNA expressionVenlafaxine treatmentDepressive disorderStress ratsDepressive symptomsIndependent cohortCalcium channelsMRNA expressionRole of potassium channels in female reproductive system
Kim J, Song K, Xu B, Wang T. Role of potassium channels in female reproductive system. Obstetrics & Gynecology Science 2020, 63: 565-576. PMID: 32838485, PMCID: PMC7494774, DOI: 10.5468/ogs.20064.Peer-Reviewed Original ResearchPotassium channelsFemale reproductive systemSulfonylurea receptorVoltage-gated (KvGonadotropin-releasing hormone neuronsEndothelium-derived hyperpolarizing factorSmall-conductance KCaHypothalamus-pituitary-ovarian axisFunction of potassium channelsReproductive systemProduction of progesteroneLuteal granulosa cellsRegulation of hormone releaseUterine quiescenceMyometrial contractionsInward rectifierSecretion of nitric oxideHormone neuronsHyperpolarizing factorResistance arteriesGranulosa cellsChannel activityHormone releaseLate pregnancySmall-conductanceSex Difference In Kidney Electrolyte Transport III: Impact of Low K intake on Thiazide‐Sensitive Cation Excretion in Male and Female Mice
Xu S, Li J, Yang L, Wang C, Liu T, Weinstein A, Palmer L, Wang T. Sex Difference In Kidney Electrolyte Transport III: Impact of Low K intake on Thiazide‐Sensitive Cation Excretion in Male and Female Mice. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.04495.Peer-Reviewed Original ResearchNa-Cl co-transporterNa/H exchanger isoform 3Glomerular filtration rateFemale miceHigh K intakeUrine volumeENaC protein expressionCation excretionDietary potassium intakeLow K intakeIncreased Na transportSex differencesNCC abundanceThiazide-sensitiveNCC expressionNa/H exchangeNa reabsorptionNHE2 expressionPotassium intakeNCC proteinDistal nephronNCC activityK intakeFiltration rateMale miceSex Differences in Maxi‐K channel and Klotho Expressions in ROMK Bartter’s Mouse Kidney
Kim J, Song K, Lee E, Xu S, Wang T. Sex Differences in Maxi‐K channel and Klotho Expressions in ROMK Bartter’s Mouse Kidney. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.04872.Peer-Reviewed Original ResearchMaxi-K channelsKO miceKlotho expressionMouse kidneyBartter's syndromeWT miceMaxi-KFemale WTRenal outer medullary potassium channelSurvival rateWT mouse kidneysUrinary potassium excretionProtein levelsBK channelsFemale mouse kidneyPotassium excretionVolume depletionROMKElectrolyte imbalancePotassium channelsBK expressionII phenotypeLife-threateningSex differencesBlood pressure
2019
Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice
Li J, Xu S, Yang L, Yang J, Wang CJ, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice. American Journal Of Physiology. Renal Physiology 2019, 317: f967-f977. PMID: 31390232, PMCID: PMC6843050, DOI: 10.1152/ajprenal.00125.2019.Peer-Reviewed Original ResearchConceptsHK dietCation excretionWT animalsFemale animalsMale animalsSex differencesRenal clearance experimentsGlomerular filtration rateR KO miceExchanger isoform 3Distal NaHK intakeWestern blot analysisFractional excretionUrine volumeFiltration rateFemale miceKO miceNHE3 abundanceClearance experimentsNCC abundanceLittle sex differenceKnockout miceENaC expressionExcretionAetiology of bipolar disorder: contribution of the L-type voltage-gated calcium channels
Guo X, Liu D, Wang T, Luo X. Aetiology of bipolar disorder: contribution of the L-type voltage-gated calcium channels. General Psychiatry 2019, 32: e100009. PMID: 31179430, PMCID: PMC6551429, DOI: 10.1136/gpsych-2018-100009.Peer-Reviewed Original ResearchImpact of High K Intake on Thiazide‐Sensitive Cation Excretion in Male and Female Wild‐type and AT1a Receptor Knockout Mice
Li J, Xu S, Wang C, Weinstein A, Palmer L, Wang T. Impact of High K Intake on Thiazide‐Sensitive Cation Excretion in Male and Female Wild‐type and AT1a Receptor Knockout Mice. The FASEB Journal 2019, 33: 864.4-864.4. DOI: 10.1096/fasebj.2019.33.1_supplement.864.4.Peer-Reviewed Original ResearchNa/H exchanger isoform 3Na-Cl co-transporterFemale wild-typeGlomerular filtration rateKO miceCation excretionNa/H exchangeDistal NaAng IIUrine volumeFemale miceThiazide-sensitive Na-Cl co-transporterApical membrane of distal tubulesAT1a receptor knockout miceHK dietWild-typeResponse to high KAT1a-KO miceK + excretionReceptor knockout miceDistal nephron segmentsFemale KO miceGroups of miceRenal clearance experimentsAT1A KOSustained‐release of hydrogen sulfide by GYY4137 protected diabetic‐induced kidney injury
Yu Y, Xu S, Ji D, Wang X, Wang T. Sustained‐release of hydrogen sulfide by GYY4137 protected diabetic‐induced kidney injury. The FASEB Journal 2019, 33: 567.2-567.2. DOI: 10.1096/fasebj.2019.33.1_supplement.567.2.Peer-Reviewed Original ResearchDKD miceDiabetic kidney diseaseWestern blottingLevels of apoptosisUntreated groupSustained-releaseUrine albuminTUNEL stainingSignaling moleculesPodocyte foot processesDiabetic kidney disease patientsAdministration of GYY4137Urinary albumin concentrationGaseous signaling moleculeProtective effectApoptosisCD-1 miceExpression of CBSBeta-lyaseExpression levelsFASEB JournalFoot processesHigh-fat dietFusion of foot processesMouse kidney
2018
Potassium conservation is impaired in mice with reduced renal expression of Kir4.1
Malik S, Lambert E, Zhang J, Wang T, Clark H, Cypress M, Goldman B, Porter GA, Pena S, Nino W, Gray D. Potassium conservation is impaired in mice with reduced renal expression of Kir4.1. American Journal Of Physiology. Renal Physiology 2018, 315: f1271-f1282. PMID: 30110571, PMCID: PMC6293297, DOI: 10.1152/ajprenal.00022.2018.Peer-Reviewed Original ResearchMeSH KeywordsAlkalosisAnimalsAquaporin 3Gene Knockdown TechniquesGenotypeHypercalcemiaHyperkalemiaHypernatremiaKidney Concentrating AbilityMice, Inbred C57BLMice, KnockoutNephronsPhenotypePhosphorylationPotassium Channels, Inwardly RectifyingPotassium, DietaryRenal ReabsorptionSolute Carrier Family 12, Member 3ConceptsMg-free dietSevere urinary concentrating defectReduced renal expressionRenal K wastingSeSAME/EAST syndromeDistal convoluted tubuleKir4.1 protein expressionWild-type miceUrinary concentrating defectCre-LoxP methodologyAquaporin 3 expressionRelative hypercalcemiaRenal expressionPotassium conservationMetabolic alkalosisNCC expressionChannel Kir4.1Distal nephronKnockout miceConvoluted tubulesCotransporter expressionEAST syndromeHypokalemiaMiceReduced expressionRegulation of renal Na transporters in response to dietary K
Yang L, Xu S, Guo X, Uchida S, Weinstein AM, Wang T, Palmer LG. Regulation of renal Na transporters in response to dietary K. American Journal Of Physiology. Renal Physiology 2018, 315: f1032-f1041. PMID: 29923764, PMCID: PMC6230734, DOI: 10.1152/ajprenal.00117.2018.Peer-Reviewed Original Research
2017
Urinary bladder hypertrophy characteristic of male ROMK Bartter’s mice does not occur in female mice
Kim JM, Xu S, Guo X, Hu H, Dong K, Wang T. Urinary bladder hypertrophy characteristic of male ROMK Bartter’s mice does not occur in female mice. AJP Regulatory Integrative And Comparative Physiology 2017, 314: r334-r341. PMID: 29092859, PMCID: PMC5899254, DOI: 10.1152/ajpregu.00315.2017.Peer-Reviewed Original ResearchConceptsKO miceBladder hypertrophyBladder weightUrinary bladder hypertrophyRenal outer medullary potassium channelSeverity of hydronephrosisWild-type miceROMK knockout miceBladder capacityDetrusor muscleWT miceUrinary tractBartter's syndromeFemale miceSalt wastingHydronephrosisKnockout miceROMK expressionMiceBladderHypertrophyPotassium channelsMRNA levelsSignificant enlargementSyndromeGender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice
Li J, Hatano R, Xu S, Wan L, Yang L, Weinstein AM, Palmer L, Wang T. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice. American Journal Of Physiology. Renal Physiology 2017, 313: f505-f513. PMID: 28566500, PMCID: PMC5582908, DOI: 10.1152/ajprenal.00087.2017.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsDiuresisFemaleHydrochlorothiazideKidneyMaleMice, KnockoutNatriuresisPhenotypeProtein Serine-Threonine KinasesReceptor, Angiotensin, Type 1Receptors, DrugSex CharacteristicsSodium Chloride SymportersSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSolute Carrier Family 12, Member 3ConceptsUrine volumeKO miceCotransporter activityRenal clearance experimentsReceptor knockout miceGlomerular filtration rateBolus intravenous injectionDelivery of NaClNCC protein expressionGender differencesFiltration rateFemale miceAT1A receptorClearance experimentsKO animalsIntravenous injectionNCC expressionDistal nephronFractional NaKnockout miceMale KOProximal tubulesHCTZMiceNHE3 expressionIntroduction to Bayesian variable selection methods in high-dimensional omics data analysis
Dong X, Xu S, Tao R, Wang T. Introduction to Bayesian variable selection methods in high-dimensional omics data analysis. Chinese Journal Of Epidemiology 2017, 38: 679-683. PMID: 28651411, DOI: 10.3760/cma.j.issn.0254-6450.2017.05.025.Peer-Reviewed Original ResearchConceptsBayesian variable selection methodOmics dataBayesian variable selectionAnalysis of high-dimensional dataDevelopment of genome sequencing technologyVariable selection methodsHigh-dimensional omics data analysisCase nGenome sequencing technologiesOmics data analysisVariable selectionVariable PHigh-dimensional dataSequencing technologiesStatistical challengesMeasure thousandsOmicsProgression of diseaseBioinformatics