2012
Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport
Du Z, Wan L, Yan Q, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f1507-f1516. PMID: 22952281, PMCID: PMC3532483, DOI: 10.1152/ajprenal.00277.2012.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAngiotensin II Type 1 Receptor BlockersAnimalsBicarbonatesBiological TransportEnzyme InhibitorsFemaleHemostasisIn Vitro TechniquesKidney GlomerulusKidney TubulesLosartanMacrolidesMiceMice, KnockoutModels, AnimalProton-Translocating ATPasesReceptor, Angiotensin, Type 1SodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsNa(+)-H(+) exchanger 3Mouse tubulesImpact of angiotensin IIH-ATPaseMicroperfused in vitroAT(2) receptorsAT(1A) receptorFlow-mediated changesH-ATPase activityParacellular Cl(-Receptor availabilityAngiotensin IIAT(1ARegulate Na(+Increased Na(+Local angiotensinBasolateral transportJ(HCO3Mouse kidneyNet fluidInhibitor bafilomycinProtein levelsMiceReceptorsTubulesRegulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport
Du Z, Yan Q, Wan L, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f386-f395. PMID: 22552936, PMCID: PMC3433864, DOI: 10.1152/ajprenal.00531.2011.Peer-Reviewed Original ResearchMeSH Keywords8-Bromo Cyclic Adenosine MonophosphateAlgorithmsAnimalsBenzazepinesBicarbonatesBiological Transport, ActiveChloridesCyclic AMP-Dependent Protein KinasesDopamineDopamine AntagonistsDopamine D2 Receptor AntagonistsFemaleIsoquinolinesKidney GlomerulusKidney TubulesKidney Tubules, ProximalMacrolidesMiceMicrovilliProton-Translocating ATPasesReceptors, Dopamine D1SodiumSulfonamidesSulpirideConceptsNa/H exchanger 3Impact of dopamineNa(+) transportNa-K-ATPase activityResponse to volume expansionProximal tubules in vitroCl(-) reabsorptionPKA-mediated mechanismMicroperfused in vitroEffects of exogenous dopamineMouse proximal tubuleNa-K-ATPaseProximal tubule reabsorptionStimulation of Cl(-PKA inhibitor H89Luminal flowH-ATPase activityTubules in vitroLuminal flow rateNHE3 activityDA1 receptorsNa/H exchangeTubule reabsorptionProximal tubulesInhibitor H89
2010
Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells
Marin EP, Moeckel G, Al-Lamki R, Bradley J, Yan Q, Wang T, Wright PL, Yu J, Sessa WC. Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells. American Journal Of Pathology 2010, 177: 2765-2773. PMID: 20971739, PMCID: PMC2993268, DOI: 10.2353/ajpath.2010.100199.Peer-Reviewed Original ResearchConceptsUnilateral ureteral obstructionAcute tubular necrosisEpithelial cellsRenal tubular epithelial cellsMurine kidneyIschemia/reperfusionMeasurement of fibrosisDistal nephron segmentsRecruitment of macrophagesWild-type miceInflammatory gene expressionTubular epithelial cellsDe novo expressionHuman biopsy specimensRenal injuryTubular necrosisUreteral obstructionWT miceVascular injuryHistological damageBiopsy specimensCortical tubulesDeficient miceMacrophage recruitmentTissue injury
2006
Use of transgenic mice in acid-base balance studies.
Cantone A, Wang T, Pica A, Simeoni M, Capasso G. Use of transgenic mice in acid-base balance studies. Journal Of Nephrology 2006, 19 Suppl 9: s121-7. PMID: 16736435.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAcid-Base ImbalanceAnimalsCation Transport ProteinsDisease Models, AnimalDNAGene ExpressionKidney TubulesMembrane ProteinsMiceMice, TransgenicNitric Oxide SynthaseSodium-Bicarbonate SymportersSodium-Hydrogen Exchanger 1Sodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsNitric oxide synthaseAcid-base statusNa+/H+ exchangeBody acid-base statusTransgenic miceFunction of pendrinProximal tubule transportTransepithelial HCO3- absorptionPotassium-chloride cotransporterEndothelial isoform of nitric oxide synthaseIsoform of nitric oxide synthaseSubunit expression levelsKnockout animal modelsApical NHE3Basolateral NHE1HCO3- absorptionNa+/HCO3- cotransporterNatriuretic responseRegulation of acid-base balanceTubule transportDistal nephronProximal tubulesRenal diseaseChloride channelsAscending limb
2004
Mechanical stimuli induce cleavage and nuclear translocation of the polycystin-1 C terminus
Chauvet V, Tian X, Husson H, Grimm DH, Wang T, Hieseberger T, Igarashi P, Bennett AM, Ibraghimov-Beskrovnaya O, Somlo S, Caplan MJ. Mechanical stimuli induce cleavage and nuclear translocation of the polycystin-1 C terminus. Journal Of Clinical Investigation 2004, 114: 1433-1443. PMID: 15545994, PMCID: PMC525739, DOI: 10.1172/jci21753.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell LineCell NucleusChlorocebus aethiopsCHO CellsCOS CellsCricetinaeCricetulusDogsEmbryo, MammalianEpithelial CellsKidney TubulesMembrane ProteinsMiceMice, TransgenicPolycystic Kidney, Autosomal DominantProteinsSequence DeletionSignal TransductionStress, MechanicalTranscription Factor AP-1TRPP Cation ChannelsConceptsC-terminal tailAutosomal dominant polycystic kidney diseaseCell-matrix interactionsCiliary signalingSecond genePolycystin-2Polycystin-1C-terminusNovel pathwayProteolytic cleavageNuclear translocationMechanical stimuliGenesDominant polycystic kidney diseasePolycystic kidney diseasePrecise mechanismCleavageTerminusSignalingTranslocationNucleusPathwayMechanosensory function of microvilli of the kidney proximal tubule
Du Z, Duan Y, Yan Q, Weinstein A, Weinbaum S, Wang T. Mechanosensory function of microvilli of the kidney proximal tubule. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 13068-13073. PMID: 15319475, PMCID: PMC516518, DOI: 10.1073/pnas.0405179101.Peer-Reviewed Original ResearchConceptsKidney proximal tubulesProximal tubulesNa+ reabsorptionNa+ transportNa+-H+ exchange activityProximal tubule Na+ reabsorptionNa+-H+ exchangeLuminal cell membraneMechanosensory functionNa+ absorptionTubular diameterLuminal diameterMouse kidneyEpithelial microvilliReabsorptionTubulesKidneyAutoregulatory mechanismNa+ uptakeHormonal systemsMiceActin cytoskeletonCell membraneTerminal webBending moment
2003
Distribution and regulation of expression of serum‐ and glucocorticoid‐induced kinase‐1 in the rat kidney
de la Rosa D, Coric T, Todorovic N, Shao D, Wang T, Canessa C. Distribution and regulation of expression of serum‐ and glucocorticoid‐induced kinase‐1 in the rat kidney. The Journal Of Physiology 2003, 551: 455-466. PMID: 12816971, PMCID: PMC2343216, DOI: 10.1113/jphysiol.2003.042903.Peer-Reviewed Original ResearchMeSH KeywordsAdrenalectomyAldosteroneAnimalsAntibodiesAntibody SpecificityBlotting, NorthernBlotting, WesternCells, CulturedDNA, ComplementaryElectrophoresis, Polyacrylamide GelEpithelial CellsGene Expression Regulation, EnzymologicGlucocorticoidsImmediate-Early ProteinsImmunoblottingIn Vitro TechniquesIsoenzymesKidneyKidney TubulesMicroscopy, FluorescenceNuclear ProteinsProtein Serine-Threonine KinasesRatsRats, Sprague-DawleyRNASubcellular FractionsTransfectionConceptsGlucocorticoid-induced kinase 1Kinase 1Ion channelsRegulation of expressionConstitutive high expressionBasolateral membraneRenal epithelial cellsSubcellular localizationLevel of expressionRegulation of levelsEpithelial ion channelsSGK1 proteinMammalian kidneyApical membraneDirect interactionSGK1Epithelial cellsWestern blottingHigh expressionExpressionExpression of SGK1ProteinRat kidneyTransportersPhysiological changes
1998
Tubule Function in Transgenic Mice
Wang T, Giebisch G. Tubule Function in Transgenic Mice. Nephron 1998, 6: 447-453. PMID: 9730661, DOI: 10.1159/000020554.Peer-Reviewed Original ResearchConceptsNa-HK-ATPaseK-ATPase expressionRenal tubule transportRenal electrolyte transportStrains of miceRenal transport functionsTransport functionColonic isoformH,K-ATPaseIsoforms NHE2Isoforms NHE1Tubule transportTubule functionTubule segmentsMouse modelTransgenic miceTransgenic knockoutTransport lesionKnockout modelsMiceKidney tubulesElectrolyte transportNull mutationTubules
1995
Effects of a novel KATP channel blocker on renal tubule function and K channel activity.
Wang T, Wang W, Klein-Robbenhaar G, Giebisch G. Effects of a novel KATP channel blocker on renal tubule function and K channel activity. Journal Of Pharmacology And Experimental Therapeutics 1995, 273: 1382-9. PMID: 7791111.Peer-Reviewed Original ResearchConceptsCortical collecting tubuleKATP channel blockerChannel blockersPotassium recyclingPotassium secretionAscending limbATP-sensitive potassium channelsActivation of KATPApical potassium conductanceReduced potassium secretionK channel activityRenal tubule functionLoop of HenleModulate NaCl reabsorptionPotassium conductanceSodium reabsorptionK secretionApical membranePrincipal cellsTubule functionPotassium channelsKATPBlockersReabsorption
1989
Neural control of distal tubular bicarbonate and fluid transport
Wang T, Chan Y. Neural control of distal tubular bicarbonate and fluid transport. American Journal Of Physiology 1989, 257: f72-f76. PMID: 2750926, DOI: 10.1152/ajprenal.1989.257.1.f72.Peer-Reviewed Original ResearchConceptsDistal convoluted tubuleConvoluted tubulesDistal tubulesRate of bicarbonate absorptionRenal nerve activityLate distal tubuleEarly distal tubuleTubular reabsorption of bicarbonateEffects of denervationReabsorption of bicarbonateRate of fluid absorptionNerve activityBicarbonate absorptionSham denervationElectrolyte excretionFluid reabsorptionDenervated kidneysReabsorb bicarbonateJHCO3DenervationFluid absorptionNeural regulationVolume markerTubulesMicroperfusion method