2013
Development of modified siRNA molecules incorporating 5-fluoro-2′-deoxyuridine residues to enhance cytotoxicity
Wu SY, Chen TM, Gmeiner WH, Chu E, Schmitz JC. Development of modified siRNA molecules incorporating 5-fluoro-2′-deoxyuridine residues to enhance cytotoxicity. Nucleic Acids Research 2013, 41: 4650-4659. PMID: 23449220, PMCID: PMC3632118, DOI: 10.1093/nar/gkt120.Peer-Reviewed Original ResearchConceptsTS proteinMultiple DNA damage repairCovalent inhibitory ternary complexNovel drug development approachDNA damage repairInhibitory ternary complexRNA stabilityDamage repairApoptotic pathwayHuman diseasesWatson-Crick base pairingPrecise fateSiRNAsControl siRNAsBase pairingTernary complexRNA expressionThymidylate synthaseMessenger RNA expressionDrug development approachesCytotoxic nucleosidesInhibitor compoundsNucleotidesSiRNAProtein
2004
Small Interfering Double-Stranded RNAs as Therapeutic Molecules to Restore Chemosensitivity to Thymidylate Synthase Inhibitor Compounds
Schmitz JC, Chen TM, Chu E. Small Interfering Double-Stranded RNAs as Therapeutic Molecules to Restore Chemosensitivity to Thymidylate Synthase Inhibitor Compounds. Cancer Research 2004, 64: 1431-1435. PMID: 14973067, DOI: 10.1158/0008-5472.can-03-1203.Peer-Reviewed Original ResearchConceptsTS expressionRKO cellsDose-dependent inhibitionCellular drug resistanceHuman colon cancer RKO cellsSynthase inhibitorColon cancer RKO cellsTherapeutic potentialDrug resistanceRNA interferenceMRNA levelsCell linesI. InhibitionProtein inductionSynthase mRNAChemosensitivityRaltitrexedDouble-stranded RNAInhibitor compoundsTherapeutic moleculesThymidylate synthase mRNATS mRNAPost-transcriptional mechanismsSiRNAEffective inhibitor