2024
Targeting hypoxia and thrombospondin‐2 in diabetic wound healing
Huang Y, Xing H, Naud S, Kyriakides T. Targeting hypoxia and thrombospondin‐2 in diabetic wound healing. The FASEB Journal 2024, 38: e70091. PMID: 39383062, PMCID: PMC11486302, DOI: 10.1096/fj.202302429rrr.Peer-Reviewed Original ResearchConceptsThrombospondin-2Diabetic miceWound healingHIF-1aMatricellular protein thrombospondin-2Diabetic woundsImpaired wound healingWounds of diabetic miceDimethyloxalylglycine treatmentTargeting hypoxiaSustained hypoxiaDiabetic patientsTSP2 expressionCell dysfunctionIncreased neovascularizationDiabetic wound healingGenetic ablationDiabetic fibroblastsElevated glucoseReduced hypoxiaImprove healingImmunofluorescence analysisHIF-1a activationHypoxiaWestern blottingPhotosensitive Nanoprobes for Rapid High Purity Isolation and Size‐Specific Enrichment of Synthetic and Extracellular Vesicle Subpopulations (Adv. Funct. Mater. 34/2024)
Weerakkody J, Tseng T, Topper M, Thoduvayil S, Radhakrishnan A, Pincet F, Kyriakides T, Gunasekara R, Ramakrishnan S. Photosensitive Nanoprobes for Rapid High Purity Isolation and Size‐Specific Enrichment of Synthetic and Extracellular Vesicle Subpopulations (Adv. Funct. Mater. 34/2024). Advanced Functional Materials 2024, 34 DOI: 10.1002/adfm.202470191.Peer-Reviewed Original ResearchVesicle biogenesisExtracellular vesicle subpopulationsLipid nanoprobesVesicle subpopulationsNative extracellular vesiclesVesicle populationsSeparate vesiclesPurity isolationExtracellular vesiclesVesiclesCellular originHydrophobic interactionsBiogenesisSize variabilitySubpopulationsIsolatesExtracellularBone-derived extracellular matrix hydrogel from thrombospondin-2 knock-out mice for bone repair
Chen Z, Zhang J, Lee F, Kyriakides T. Bone-derived extracellular matrix hydrogel from thrombospondin-2 knock-out mice for bone repair. Acta Biomaterialia 2024, 186: 85-94. PMID: 39134130, PMCID: PMC11500023, DOI: 10.1016/j.actbio.2024.08.011.Peer-Reviewed Original ResearchECM hydrogelsWild typeBone repairLack of mechanical integrityExtracellular matrixRepair of bone defectsExtracellular matrix hydrogelDecellularized extracellular matrixMurine calvarial defect modelCalvarial defect modelPromote bone repairHuman umbilical vein endothelial cellsMesenchymal stem cellsThrombospondin-2Fabricated hydrogelsBone extracellular matrixNovel hydrogelsMechanical propertiesCollagen fibril assemblyMatrix hydrogelRepair damaged boneDecellularized ECMMechanical integrityHydrogelsInvasion in vitroPhotosensitive Nanoprobes for Rapid High Purity Isolation and Size‐Specific Enrichment of Synthetic and Extracellular Vesicle Subpopulations
Weerakkody J, Tseng T, Topper M, Thoduvayil S, Radhakrishnan A, Pincet F, Kyriakides T, Gunasekara R, Ramakrishnan S. Photosensitive Nanoprobes for Rapid High Purity Isolation and Size‐Specific Enrichment of Synthetic and Extracellular Vesicle Subpopulations. Advanced Functional Materials 2024, 34 PMID: 39372670, PMCID: PMC11452007, DOI: 10.1002/adfm.202400390.Peer-Reviewed Original ResearchExtracellular vesicle subpopulationsVesicle subpopulationsIsolation of vesiclesPurity extracellular vesiclesRelease of vesiclesAnalysis of nucleic acidsNear-native formLarge-scale isolationLipid nanoprobesDownstream analysisPurity isolationEfficient isolationVesiclesSynthetic vesiclesNucleic acidsExtracellular vesiclesIsolation methodIsolatesBiomarker discoveryExposure to lightSubpopulationsEnrichmentProteinComplex biological mediaCleavageEditorial: Modulation of the Immune System to Improve Tissue Regeneration Strategies
Bracaglia L, Kyriakides T. Editorial: Modulation of the Immune System to Improve Tissue Regeneration Strategies. Tissue Engineering Part A 2024, 30: 257-258. PMID: 38451538, DOI: 10.1089/ten.tea.2024.29055.editorial.Peer-Reviewed Original ResearchNovel muscle-derived extracellular matrix hydrogel promotes angiogenesis and neurogenesis in volumetric muscle loss
Chen Z, Huang Y, Xing H, Tseng T, Edelman H, Perry R, Kyriakides T. Novel muscle-derived extracellular matrix hydrogel promotes angiogenesis and neurogenesis in volumetric muscle loss. Matrix Biology 2024, 127: 38-47. PMID: 38325441, PMCID: PMC10958762, DOI: 10.1016/j.matbio.2024.02.001.Peer-Reviewed Original ResearchConceptsDecellularized extracellular matrixVolumetric muscle lossCharacterization of hydrogelsExtracellular matrix hydrogelTreat VML injuriesMuscle lossEnhancement of tissue repairRegenerative capacity of skeletal muscleMatrix hydrogelPromote muscle repairRegenerative capacityHydrogelsVolumetric muscle loss modelNative biomaterialsScar tissue formationEnhanced cell invasionCapacity of skeletal muscleMuscle repairTibialis anterior muscleClinical challengeBlood vessel formationReduce inflammationVML injuryLoss of functionSubcutaneous implantation
2023
Call for Papers: Special Issue on Modulation of the Immune System to Improve Tissue Regeneration Strategies
Bracaglia L, Kyriakides T. Call for Papers: Special Issue on Modulation of the Immune System to Improve Tissue Regeneration Strategies. Tissue Engineering Part B Reviews 2023, 29: 189-189. DOI: 10.1089/ten.teb.2023.29018.cfp.Peer-Reviewed Original ResearchCall for Papers: Special Issue on Modulation of the Immune System to Improve Tissue Regeneration Strategies
Bracaglia L, Kyriakides T. Call for Papers: Special Issue on Modulation of the Immune System to Improve Tissue Regeneration Strategies. Tissue Engineering Part C Methods 2023 PMID: 37222726, DOI: 10.1089/tec.2023.29038.cfp.Peer-Reviewed Original ResearchAuthor Correction: Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction
Xing H, Huang Y, Kunkemoeller B, Dahl P, Muraleetharan O, Malvankar N, Murrell M, Kyriakides T. Author Correction: Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction. Scientific Reports 2023, 13: 4253. PMID: 36918662, PMCID: PMC10015071, DOI: 10.1038/s41598-023-31191-w.Peer-Reviewed Original Research
2022
Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction
Xing H, Huang Y, Kunkemoeller B, Dahl P, Muraleetharan O, Malvankar N, Murrell M, Kyriakides T. Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction. Scientific Reports 2022, 12: 22474. PMID: 36577792, PMCID: PMC9797577, DOI: 10.1038/s41598-022-26337-1.Peer-Reviewed Original ResearchConceptsCell-derived matricesCell stiffnessFamily verprolin-homologous protein 2Active Rac1Thrombospondin-2Homologous protein 2Less traction forceCytoskeleton organizationExtracellular matrix productionMajor cell populationF-actinCytoskeletal disorganizationRegulatory roleProtein 2Matrix productionCritical functionsECM productionArt microscopy techniquesNew functionsCell populationsSpindle-like shapeRac1Normal fibroblastsFibroblastsWound spaceTreating ‘Septic’ With Enhanced Antibiotics and ‘Arthritis’ by Mitigation of Excessive Inflammation
Kwon HK, Dussik CM, Kim SH, Kyriakides TR, Oh I, Lee FY. Treating ‘Septic’ With Enhanced Antibiotics and ‘Arthritis’ by Mitigation of Excessive Inflammation. Frontiers In Cellular And Infection Microbiology 2022, 12: 897291. PMID: 35755835, PMCID: PMC9218192, DOI: 10.3389/fcimb.2022.897291.Peer-Reviewed Original ResearchConceptsSeptic arthritisBacterial burdenAntibiotic treatmentMurine modelTherapeutic goalsConcurrent antimicrobial therapyDistinct therapeutic goalsGeneration of inflammationMRSA septic arthritisSeptic knee arthritisInflammatory joint conditionsArticular cartilageMitigation of inflammationPost-antibiotic treatmentNovel therapeutic strategiesSeptic arthritis modelArticular cartilage damageEx vivo modelArticular cartilage integrityInflammatory arthritisInhibitors of ERKInflammatory profileMRSA infectionSynovial tissueExcessive inflammationForeign body response to synthetic polymer biomaterials and the role of adaptive immunity
Kyriakides TR, Kim HJ, Zheng C, Harkins L, Tao W, Deschenes E. Foreign body response to synthetic polymer biomaterials and the role of adaptive immunity. Biomedical Materials 2022, 17: 022007. PMID: 35168213, PMCID: PMC9159526, DOI: 10.1088/1748-605x/ac5574.Peer-Reviewed Original ResearchConceptsAdaptive immune cellsForeign body reactionImmune cellsAdaptive immunityImmune-inflammatory cellsSpecific macrophage subsetsSpecific antibody responseForeign body giant cellsWound repair cellsInflammatory cellsAntibody responseFavorable outcomeMacrophage subtypesDistinct immuneMacrophage subsetsB cellsGiant cellsRegenerative responseBody reactionRepair cellsImmunityForeign body responseExact participationBody responseContradictory studies
2021
Integrin β3 targeting biomaterial preferentially promotes secretion of bFGF and viability of iPSC-derived vascular smooth muscle cells
Dash BC, Duan K, Kyriakides TR, Hsia HC. Integrin β3 targeting biomaterial preferentially promotes secretion of bFGF and viability of iPSC-derived vascular smooth muscle cells. Biomaterials Science 2021, 9: 5319-5329. PMID: 34190227, DOI: 10.1039/d1bm00162k.Peer-Reviewed Original ResearchConceptsBasic fibroblast growth factorVascular smooth muscle cellsSmooth muscle cellsIntegrin β3Paracrine secretionMuscle cellsGrowth factorSecretion of bFGFMatrix metalloproteinase-2Proangiogenic growth factorsCell viabilityFibroblast growth factorHuman-induced pluripotent stemMetalloproteinase-2Ligand-integrin interactionExtracellular matrix microenvironmentSecretionPossible rolePositive feedback loopPaucity of researchMatrix microenvironmentIntegrin interactionPluripotent stemFibrillar collagenΒ3Dual therapeutic targeting of intra-articular inflammation and intracellular bacteria enhances chondroprotection in septic arthritis
Kwon HK, Lee I, Yu KE, Cahill SV, Alder KD, Lee S, Dussik CM, Back J, Choi J, Song L, Kyriakides TR, Lee FY. Dual therapeutic targeting of intra-articular inflammation and intracellular bacteria enhances chondroprotection in septic arthritis. Science Advances 2021, 7: eabf2665. PMID: 34172438, PMCID: PMC8232912, DOI: 10.1126/sciadv.abf2665.Peer-Reviewed Original ResearchConceptsIntra-articular inflammationSeptic arthritisBacterial infectionsDual therapeutic targetingKnee septic arthritisPromising new therapeutic strategyArticular cartilageChallenging clinical problemNovel treatment modalitiesIntracellular bacteriaNew therapeutic strategiesAdjuvant targetingTreatment modalitiesBacterial eradicationSuccessful treatmentHost inflammationCausative bacteriaMurine modelTherapeutic strategiesArthritisTherapeutic goalsClinical problemTherapeutic targetingInflammationVital organsBiocompatibility of platinum-based bulk metallic glass in orthopedic applications
Loye AM, Kwon HK, Dellal D, Ojeda R, Lee S, Davis R, Nagle N, Doukas PG, Schroers J, Lee FY, Kyriakides TR. Biocompatibility of platinum-based bulk metallic glass in orthopedic applications. Biomedical Materials 2021, 16: 045018. PMID: 33873168, DOI: 10.1088/1748-605x/abf981.Peer-Reviewed Original ResearchConceptsBulk metallic glassPt-BMGPlatinum-based bulk metallic glassMetallic glassesConventional metallic implantsCorrosion resistanceHigh strengthMechanical testingOrthopedic applicationsBone applicationsMetallic implantsMicro-computed tomographyAmorphous metalsTitaniumWearBiocompatibilitySimilar biocompatibilityFurther processingGlassDuctilityNanopatternsNanoscaleApplicationsMesenchymal stem cellsStrengthBiocompatibility of nanomaterials and their immunological properties
Kyriakides TR, Raj A, Tseng TH, Xiao H, Nguyen R, Mohammed FS, Halder S, Xu M, Wu MJ, Bao S, Sheu WC. Biocompatibility of nanomaterials and their immunological properties. Biomedical Materials 2021, 16: 042005. PMID: 33578402, PMCID: PMC8357854, DOI: 10.1088/1748-605x/abe5fa.Peer-Reviewed Original ResearchConceptsMetal/metal oxideBiocompatibility of nanomaterialsNanomaterialsNovel sensingMetal oxidesBiocompatibilityOverall biocompatibilityBulk materialClinical trialsOccupational exposureTherapeutic approachesSystemic effectsNanotoxicityClinical useFabricationPutative negative effectsImmunological propertiesSensingCell typesOxideLiposomesHost proteinsReciprocal interactionsApplicationsCarbonLoss of endothelial glucocorticoid receptor promotes angiogenesis via upregulation of Wnt/β-catenin pathway
Liu B, Zhou H, Zhang T, Gao X, Tao B, Xing H, Zhuang Z, Dardik A, Kyriakides TR, Goodwin JE. Loss of endothelial glucocorticoid receptor promotes angiogenesis via upregulation of Wnt/β-catenin pathway. Angiogenesis 2021, 24: 631-645. PMID: 33650028, PMCID: PMC8292305, DOI: 10.1007/s10456-021-09773-x.Peer-Reviewed Original ResearchConceptsWnt/β-catenin signalingWnt/β-catenin pathwayΒ-catenin signalingΒ-catenin pathwayAutophagy fluxKey biological processesGlucocorticoid receptorNuclear receptor familyTube formation assaysEndothelial cellsBiological processesCanonical WntP62 degradationReceptor familyFormation assaysAbsence of GRCell viability assaysProcess of angiogenesisWntGR regulationSignalingVivo assaysQuantitative PCRKey receptorPathwayLocally delivered adjuvant biofilm‐penetrating antibiotics rescue impaired endochondral fracture healing caused by MRSA infection
Cahill SV, Kwon H, Back J, Lee I, Lee S, Alder KD, Hao Z, Yu KE, Dussik CM, Kyriakides T, Lee F. Locally delivered adjuvant biofilm‐penetrating antibiotics rescue impaired endochondral fracture healing caused by MRSA infection. Journal Of Orthopaedic Research® 2021, 39: 402-414. PMID: 33336805, DOI: 10.1002/jor.24965.Peer-Reviewed Original ResearchConceptsHigh-dose rifampinFracture healingMRSA infectionMethicillin-resistant Staphylococcus aureus osteomyelitisColony-forming unitsTissue bacterial loadStaphylococcus aureus osteomyelitisReactive bone formationRadiographic union scaleEndochondral fracture healingBacterial CFU countsPoor callus formationSoft callus formationTibia scoresRifampin therapyDevastating complicationMRSA colonizationClinical efficacyInfected fracturesTibial radiographsTreatment responseMurine modelIntramedullary pinOpen fracturesO stain
2020
An in situ collagen‐HA hydrogel system promotes survival and preserves the proangiogenic secretion of hiPSC‐derived vascular smooth muscle cells
Dash BC, Duan K, Xing H, Kyriakides TR, Hsia HC. An in situ collagen‐HA hydrogel system promotes survival and preserves the proangiogenic secretion of hiPSC‐derived vascular smooth muscle cells. Biotechnology And Bioengineering 2020, 117: 3912-3923. PMID: 32770746, DOI: 10.1002/bit.27530.Peer-Reviewed Original ResearchThe role of extracellular matrix in the pathophysiology of diabetic wounds
Huang Y, Kyriakides TR. The role of extracellular matrix in the pathophysiology of diabetic wounds. Matrix Biology Plus 2020, 6: 100037. PMID: 33543031, PMCID: PMC7852307, DOI: 10.1016/j.mbplus.2020.100037.Peer-Reviewed Original ResearchECM-modifying enzymesExtracellular matrix productionDevelopment of ECMDiabetic woundsStructural proteinsECM moleculesKey ECM moleculesExtracellular matrixMatricellular proteinDiabetes-induced changesMatrix productionImpaired wound healingUlcerated woundsNew insightsProteinImpaired healingTissue dysfunctionTherapeutic strategiesWound healingWoundsHealingEnzyme