2024
Photosensitive Nanoprobes for Rapid High Purity Isolation and Size‐Specific Enrichment of Synthetic and Extracellular Vesicle Subpopulations
Weerakkody J, Tseng T, Topper M, Thoduvayil S, Radhakrishnan A, Pincet F, Kyriakides T, Gunasekara R, Ramakrishnan S. Photosensitive Nanoprobes for Rapid High Purity Isolation and Size‐Specific Enrichment of Synthetic and Extracellular Vesicle Subpopulations. Advanced Functional Materials 2024, 34 PMID: 39372670, PMCID: PMC11452007, DOI: 10.1002/adfm.202400390.Peer-Reviewed Original ResearchExtracellular vesicle subpopulationsVesicle subpopulationsIsolation of vesiclesPurity extracellular vesiclesRelease of vesiclesAnalysis of nucleic acidsNear-native formLarge-scale isolationLipid nanoprobesDownstream analysisPurity isolationEfficient isolationVesiclesSynthetic vesiclesNucleic acidsExtracellular vesiclesIsolation methodIsolatesBiomarker discoveryExposure to lightSubpopulationsEnrichmentProteinComplex biological mediaCleavage
2020
The role of extracellular matrix in the pathophysiology of diabetic wounds
Huang Y, Kyriakides TR. The role of extracellular matrix in the pathophysiology of diabetic wounds. Matrix Biology Plus 2020, 6: 100037. PMID: 33543031, PMCID: PMC7852307, DOI: 10.1016/j.mbplus.2020.100037.Peer-Reviewed Original ResearchECM-modifying enzymesExtracellular matrix productionDevelopment of ECMDiabetic woundsStructural proteinsECM moleculesKey ECM moleculesExtracellular matrixMatricellular proteinDiabetes-induced changesMatrix productionImpaired wound healingUlcerated woundsNew insightsProteinImpaired healingTissue dysfunctionTherapeutic strategiesWound healingWoundsHealingEnzyme
2019
Thrombospondin-2 regulates extracellular matrix production, LOX levels, and cross-linking via downregulation of miR-29
Calabro NE, Barrett A, Chamorro-Jorganes A, Tam S, Kristofik NJ, Xing H, Loye AM, Sessa WC, Hansen K, Kyriakides TR. Thrombospondin-2 regulates extracellular matrix production, LOX levels, and cross-linking via downregulation of miR-29. Matrix Biology 2019, 82: 71-85. PMID: 30876926, PMCID: PMC6710120, DOI: 10.1016/j.matbio.2019.03.002.Peer-Reviewed Original ResearchConceptsECM protein productionProtein productionLysyl oxidaseThrombospondin-2MiR-29Matricellular proteinQuantitative proteomics approachLevels of LOXExtracellular matrix productionProteomic approachECM proteinsMajor regulatorECM homeostasisExtracellular matrixMatrix productionProteinCollagen fibrillogenesisMiR-29 expressionFibrillar collagenImportant modulatorDependent processesLOX levelsRegulatorProductionHomeostasis
2015
Chapter 5 Molecular Events at Tissue–Biomaterial Interface
Kyriakides T. Chapter 5 Molecular Events at Tissue–Biomaterial Interface. 2015, 81-116. DOI: 10.1016/b978-0-12-800196-7.00005-0.Peer-Reviewed Original ResearchMolecular eventsIntracellular molecular pathwaysInteraction of proteinsKey inflammatory cell typeProduction of signalsAssociated proteinsProtein altersAltered conformationTissue-biomaterial interfaceConformational changesCell adhesionMolecular pathwaysCell typesProteinProteinaceous coatingsCell functionMolecular interactionsCell receptorCurrent understandingBiomaterial interactionsMatrix depositionActivationEventual encapsulationResident cellsCells