2015
The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome–mediated inflammatory disease
Youm YH, Nguyen KY, Grant RW, Goldberg EL, Bodogai M, Kim D, D'Agostino D, Planavsky N, Lupfer C, Kanneganti TD, Kang S, Horvath TL, Fahmy TM, Crawford PA, Biragyn A, Alnemri E, Dixit VD. The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome–mediated inflammatory disease. Nature Medicine 2015, 21: 263-269. PMID: 25686106, PMCID: PMC4352123, DOI: 10.1038/nm.3804.Peer-Reviewed Original Research
2006
Uncoupling protein 2/3 immunoreactivity and the ascending dopaminergic and noradrenergic neuronal systems: Relevance for volume transmission
Rivera A, Agnati LF, Horvath TL, Valderrama JJ, de La Calle A, Fuxe K. Uncoupling protein 2/3 immunoreactivity and the ascending dopaminergic and noradrenergic neuronal systems: Relevance for volume transmission. Neuroscience 2006, 137: 1447-1461. PMID: 16387447, DOI: 10.1016/j.neuroscience.2005.05.051.Peer-Reviewed Original ResearchConceptsConfocal laser microscopy analysisReactive oxygen species productionLaser microscopy analysisProtein 2/3Oxygen species productionUncouple oxidative phosphorylationOxidative phosphorylationATP synthesisProteinSpecies productionDouble immunolabelingImportant roleMicroscopy analysisPhosphorylationMitochondriaRegulationCell groupsPlastic changesLocalizationIslandsAnimal modelsMagnaTyrosine hydroxylaseNeuronal systems
2005
Mitochondrial uncoupling proteins in the cns: in support of function and survival
Andrews ZB, Diano S, Horvath TL. Mitochondrial uncoupling proteins in the cns: in support of function and survival. Nature Reviews Neuroscience 2005, 6: 829-840. PMID: 16224498, DOI: 10.1038/nrn1767.Peer-Reviewed Original ResearchConceptsNeuronal functionNeurological disordersTraumatic brain injuryAmyotrophic lateral sclerosisClinical treatment strategiesMitochondrial calcium influxModels of neurodegenerationMitochondrial uncouplingFree radical productionReactive oxygen species productionNeuronal deteriorationNeuronal deathSubstantia nigraBrain injurySpinal cordVentral tegmentumTreatment strategiesOxygen species productionNeuronal microenvironmentSynaptic transmissionCalcium influxLimbic systemNeurological conditionsLateral sclerosisParkinson's disease
2003
Fasting Activates the Nonhuman Primate Hypocretin (Orexin) System and Its Postsynaptic Targets
Diano S, Horvath B, Urbanski HF, Sotonyi P, Horvath TL. Fasting Activates the Nonhuman Primate Hypocretin (Orexin) System and Its Postsynaptic Targets. Endocrinology 2003, 144: 3774-3778. PMID: 12933647, DOI: 10.1210/en.2003-0274.Peer-Reviewed Original ResearchConceptsPerifornical regionArcuate nucleusHypothalamic regulationC-Fos-expressing cellsLateral hypothalamic cellsC-fosMedial preoptic areaVariety of endocrineC-fos expressionDiverse brain regionsHCRT axonsLeptin levelsNuclear c-FosDorsomedial hypothalamusAsymmetric synapsesBasal forebrainPresent studyPostsynaptic targetsDorsomedial nucleusNeuropeptide Y.Preoptic areaThalamic nucleiHcrt neuronsHcrt systemAxon terminalsMitochondrial uncoupling protein 2 in the central nervous system: neuromodulator and neuroprotector
Horvath TL, Diano S, Barnstable C. Mitochondrial uncoupling protein 2 in the central nervous system: neuromodulator and neuroprotector. Biochemical Pharmacology 2003, 65: 1917-1921. PMID: 12787871, DOI: 10.1016/s0006-2952(03)00143-6.Peer-Reviewed Original ResearchConceptsMitochondrial membrane potentialInner membraneRole of UCP2Normal neuronal functionDiverse tissuesUnknown functionProtein 2Functional significanceImportant playersCentral nervous systemNeuronal functionProteinMembrane potentialNervous systemUCP2Disease statesUCPBrown adipose tissueNew avenuesMitochondriaTissueAdipose tissueUCP1Better understandingUncouplerUncoupling proteins-2 and 3 influence obesity and inflammation in transgenic mice
Horvath TL, Diano S, Miyamoto S, Barry S, Gatti S, Alberati D, Livak F, Lombardi A, Moreno M, Goglia F, Mor G, Hamilton J, Kachinskas D, Horwitz B, Warden CH. Uncoupling proteins-2 and 3 influence obesity and inflammation in transgenic mice. International Journal Of Obesity 2003, 27: 433-442. PMID: 12664076, DOI: 10.1038/sj.ijo.0802257.Peer-Reviewed Original ResearchMeSH KeywordsAdipose TissueAnimalsBasal MetabolismBlotting, NorthernBlotting, WesternBody TemperatureCarrier ProteinsCholesterol, LDLEnergy IntakeGene Expression RegulationHeart RateInflammationIon ChannelsMaleMembrane Transport ProteinsMiceMice, Inbred C57BLMice, TransgenicMitochondriaMitochondrial ProteinsObesityProteinsUncoupling Protein 2Uncoupling Protein 3ConceptsTransgenic miceFat massLow-density lipoprotein cholesterol levelsHeterozygous miceAgouti obese miceHypothalamic neuropeptide levelsSpontaneous physical activityLipoprotein cholesterol levelsNontransgenic littermate controlsFat pad weightEndotoxin-induced feverWild-type littermatesHuman UCP2Significant differencesMechanism of actionLDL cholesterolControl miceFemale transgenicsNontransgenic littermatesObese miceEndotoxin injectionCholesterol levelsPad weightNeuropeptide levelsFood intakeMitochondrial Uncoupling Proteins: Regulators of Retinal Cell Death
Barnstable CJ, Li M, Reddy R, Horvath TL. Mitochondrial Uncoupling Proteins: Regulators of Retinal Cell Death. Advances In Experimental Medicine And Biology 2003, 533: 269-275. PMID: 15180273, DOI: 10.1007/978-1-4615-0067-4_33.Peer-Reviewed Original ResearchConceptsOxidative stressElevated levelsOptic nerve disordersGanglion cell deathCell deathRetinal cell deathSuch elevated levelsNerve disordersInner retinaNeuronal degenerationReactive oxygen radicalsMetabolic conditionsNeural componentsApoptotic cell deathOxygen radicalsIntracellular metabolismDeathSpecific mutationsCell typesExtensive evidenceIrreversible cascadeGlaucomaDegenerationRetina
2000
Evidence of NPY Y5 receptor involvement in food intake elicited by orexin A in sated rats
Dube M, Horvath T, Kalra P, Kalra S. Evidence of NPY Y5 receptor involvement in food intake elicited by orexin A in sated rats. Peptides 2000, 21: 1557-1560. PMID: 11068104, DOI: 10.1016/s0196-9781(00)00311-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAppetite DepressantsAppetite RegulationCarrier ProteinsEatingFeeding BehaviorInjections, IntraventricularIntracellular Signaling Peptides and ProteinsMaleNaphthalenesNerve Tissue ProteinsNeuronsNeuropeptidesOrexin ReceptorsOrexinsPyrimidinesRatsRats, Sprague-DawleyReceptors, G-Protein-CoupledReceptors, NeuropeptideReceptors, Neuropeptide YSignal TransductionWeight GainConceptsOrexigenic peptideReceptor antagonistSated ratsNPY Y1 receptor antagonistNPY-producing neuronsPotent orexigenic peptideNPY Y5 receptor antagonistsY1 receptor antagonistY5 receptor antagonistsOrexin AIntracerebroventricular injectionY5 receptorsNeuropeptide YReceptor involvementFood intakeStimulate feedingNeuronsAntagonistRatsFeedingOrexinCurrent resultsFunctional linkHypothalamusNPYEvidence that NPY Y1 receptors are involved in stimulation of feeding by orexins (hypocretins) in sated rats
Jain M, Horvath T, Kalra P, Kalra S. Evidence that NPY Y1 receptors are involved in stimulation of feeding by orexins (hypocretins) in sated rats. Peptides 2000, 87: 19-24. PMID: 10710284, DOI: 10.1016/s0167-0115(99)00102-0.Peer-Reviewed Original ResearchConceptsNeuropeptide YLateral hypothalamic areaArcuate nucleusY1 receptorParaventricular nucleusSelective NPY Y1 receptor antagonistNPY Y1 receptor antagonistNPY Y1 receptorY1 receptor antagonistStimulation of feedingAdult male ratsDose-dependent mannerOrexigenic peptideOrexin ANPY receptorsExcitatory effectsHypothalamic areaHypothalamic appetiteReceptor antagonistMale ratsSated ratsOrexinNeural sitesReceptorsNPYergic
1999
Synaptic Interaction between Hypocretin (Orexin) and Neuropeptide Y Cells in the Rodent and Primate Hypothalamus: A Novel Circuit Implicated in Metabolic and Endocrine Regulations
Horvath T, Diano S, van den Pol A. Synaptic Interaction between Hypocretin (Orexin) and Neuropeptide Y Cells in the Rodent and Primate Hypothalamus: A Novel Circuit Implicated in Metabolic and Endocrine Regulations. Journal Of Neuroscience 1999, 19: 1072-1087. PMID: 9920670, PMCID: PMC6782143, DOI: 10.1523/jneurosci.19-03-01072.1999.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsChlorocebus aethiopsEndocrine GlandsFemaleHypothalamusIntracellular Signaling Peptides and ProteinsMaleMetabolismNeural PathwaysNeuronsNeuropeptide YNeuropeptidesOrexin ReceptorsOrexinsRatsRats, Sprague-DawleyReceptors, Cell SurfaceReceptors, G-Protein-CoupledReceptors, LeptinReceptors, NeuropeptideSynapsesConceptsHypothalamic functionCentral regulationHypocretin-containing neuronsLateral hypothalamic cellsLeptin receptor immunoreactivityNeuropeptide Y cellsDirect synaptic contactsNeuropeptide Y systemEndocrine regulationEndocrine processesNPY releaseReceptor immunoreactivityExcitatory actionHypocretin cellsSynaptic contactsArcuate nucleusLateral hypothalamusPrimate hypothalamusLeptin receptorSame neuronsHypothalamic cellsSynaptic regulationAdipose tissueHypocretinNPY
1998
Leptin receptors in estrogen receptor-containing neurons of the female rat hypothalamus
Diano S, Kalra S, Sakamoto H, Horvath T. Leptin receptors in estrogen receptor-containing neurons of the female rat hypothalamus. Brain Research 1998, 812: 256-259. PMID: 9813356, DOI: 10.1016/s0006-8993(98)00936-6.Peer-Reviewed Original ResearchConceptsLeptin receptorEstrogen receptorNeuronal perikaryaPeripheral signalsEstrogen receptor-containing neuronsParvicellular paraventricular nucleusReceptor-containing neuronsMedial preoptic areaVentromedial hypothalamic nucleusFemale rat hypothalamusArcuate nucleusHypothalamic nucleiFemale ratsParaventricular nucleusPreoptic areaGonadal functionHypothalamic sectionsPeriventricular regionRat hypothalamusNeuroendocrine mechanismsReceptorsExtensive colocalizationLeptinHypothalamusPerikaryaLeptin Receptor Immunoreactivity is Associated with the Golgi Apparatus of Hypothalamic Neurones and Glial Cells
Diano S, Kalra S, Horvath T. Leptin Receptor Immunoreactivity is Associated with the Golgi Apparatus of Hypothalamic Neurones and Glial Cells. Journal Of Neuroendocrinology 1998, 10: 647-650. PMID: 9744481, DOI: 10.1046/j.1365-2826.1998.00261.x.Peer-Reviewed Original ResearchConceptsLeptin receptor immunoreactivityReceptor immunoreactivityPerikaryal membraneGlial cellsGolgi apparatusHypothalamic neuronesCentral nervous systemDifferent second messenger systemsHypothalamic neuronalPeripheral hormonesDentate gyrusSecond messenger systemsEndocrine functionLeptin receptorHypothalamic cellsNervous systemThyroid axisImmunoreactivityCentral mechanismsLeptinIntracellular mechanismsTrans cisternaeNeuronesMessenger systemsPredominant localization