2001
Male germ cells and photoreceptors, both dependent on close cell–cell interactions, degenerate upon ClC‐2 Cl− channel disruption
Bösl M, Stein V, Hübner C, Zdebik A, Jordt S, Mukhopadhyay A, Davidoff M, Holstein A, Jentsch T. Male germ cells and photoreceptors, both dependent on close cell–cell interactions, degenerate upon ClC‐2 Cl− channel disruption. The EMBO Journal 2001, 20: 1289-1299. PMID: 11250895, PMCID: PMC145530, DOI: 10.1093/emboj/20.6.1289.Peer-Reviewed Original ResearchConceptsClC-2Abnormal Sertoli cellsBlood-retina barrierGerm cellsRetinal pigment epitheliumCl- channelsSevere degenerationMale infertilityPigment epitheliumMale germ cellsClC-2 Cl(-) channelOvert abnormalitiesSeminiferous tubulesNormal outer segmentsSertoli cellsOuter segmentsRetinaCell typesDeathMassive deathCellsPrimary spermatocytesHuman diseasesTubulesCLC Cl(-) channels
1999
Chloride dependence of hyperpolarization‐activated chloride channel gates
Pusch M, Jordt S, Stein V, Jentsch T. Chloride dependence of hyperpolarization‐activated chloride channel gates. The Journal Of Physiology 1999, 515: 341-353. PMID: 10050002, PMCID: PMC2269146, DOI: 10.1111/j.1469-7793.1999.341ac.x.Peer-Reviewed Original Research
1998
Characterization of the hyperpolarization‐activated chloride current in dissociated rat sympathetic neurons
Clark S, Jordt S, Jentsch T, Mathie A. Characterization of the hyperpolarization‐activated chloride current in dissociated rat sympathetic neurons. The Journal Of Physiology 1998, 506: 665-678. PMID: 9503329, PMCID: PMC2230754, DOI: 10.1111/j.1469-7793.1998.665bv.x.Peer-Reviewed Original ResearchConceptsSCG neuronsRat superior cervical ganglion neuronsChloride currentsSuperior cervical ganglion neuronsConventional whole-cell recordingRat SCG neuronsClC-2Rat cerebellar granule cellsRat sympathetic neuronsPerforated-patch recordingsWhole-cell recordingsHyperpolarization-activated chloride currentCerebellar granule cellsTranscriptase-polymerase chain reaction experimentsPresence of mRNAGanglion neuronsSympathetic neuronsGABAA receptorsMM cesiumGranule cellsEffective blockerExternal potassium concentrationCl- currentInternal chloride concentrationHyperpolarization-activated Cl- current
1997
Molecular dissection of gating in the ClC‐2 chloride channel
Jordt S, Jentsch T. Molecular dissection of gating in the ClC‐2 chloride channel. The EMBO Journal 1997, 16: 1582-1592. PMID: 9130703, PMCID: PMC1169762, DOI: 10.1093/emboj/16.7.1582.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell MembraneChloride ChannelsConsensus SequenceHydrogen-Ion ConcentrationIon Channel GatingMembrane PotentialsModels, StructuralMolecular Sequence DataMutagenesis, Site-DirectedOocytesPatch-Clamp TechniquesPoint MutationProtein Structure, SecondaryRatsRecombinant Fusion ProteinsXenopus laevisConceptsChloride channelsClC-2 chloride channelSite-directed mutagenesisMolecular dissectionEquivalent mutationCytoplasmic loopMode of activationClC-0ClC-2Gating mechanismInactivation domainMutationsCell swellingActivationNeuronal excitabilityMutagenesisTransmembraneCell volumeDeletionRegulationPathwayFinal pathwayMechanismVoltage dependenceOutward rectification