2016
Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis
Robson A, Owens ND, Baserga SJ, Khokha MK, Griffin JN. Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis. BMC Developmental Biology 2016, 16: 38. PMID: 27784267, PMCID: PMC5081970, DOI: 10.1186/s12861-016-0138-5.Peer-Reviewed Original ResearchConceptsRibosome biogenesis factorsBiogenesis factorsVentral blood islandsHuman congenital diseasesCranial neural crestRNA-seq dataAntisense mRNA probesBlood islandsEmbryonic developmentDistinct tissuesNeural crestProtein productionDifferential expressionTranscript numbersMachinery componentsSpecific tissuesSpecific phenotypesRibosomopathiesSitu hybridizationMRNA probesRibosomesExpressionTissue localizationPhenotypeFactor expressionThe Contributions of the Ribosome Biogenesis Protein Utp5/WDR43 to Craniofacial Development
Sondalle SB, Baserga SJ, Yelick PC. The Contributions of the Ribosome Biogenesis Protein Utp5/WDR43 to Craniofacial Development. Journal Of Dental Research 2016, 95: 1214-1220. PMID: 27221611, PMCID: PMC5076753, DOI: 10.1177/0022034516651077.Peer-Reviewed Original ResearchConceptsRibosomal proteinsCraniofacial developmentRibosome biogenesis proteinsRibosomal protein functionHuman craniofacial developmentTissue-specific defectsDiverse cell typesHuman ribosomopathiesBiogenesis proteinsTranslational machineryProtein functionDistinct functionsTranslational mechanismsTissue differentiationCell typesWDR43Global defectsProteinExciting researchSurprising similaritiesUnderstanding of rolesRibosomopathiesBiogenesisMachineryMutations
2015
Determinants of mammalian nucleolar architecture
Farley KI, Surovtseva Y, Merkel J, Baserga SJ. Determinants of mammalian nucleolar architecture. Chromosoma 2015, 124: 323-331. PMID: 25670395, PMCID: PMC4534358, DOI: 10.1007/s00412-015-0507-z.Peer-Reviewed Original ResearchConceptsNucleolar formationNucleolar architectureNucleolar structureNucleolar organizer regionsProduction of ribosomesRibosome biogenesisFunctional nucleoliHuman nucleoliCell divisionOrganizer regionsNucleoliEssential machineFunction manifestBiogenesisIntricate relationshipMinimal requirementsCellsRibosomesOrganellesProteinFunctionFormationDivisionDeterminants
2001
Fibrillarin and Other snoRNP Proteins Are Targets of Autoantibodies in Xenobiotic-Induced Autoimmunity
Yang J, Baserga S, Turley S, Pollard K. Fibrillarin and Other snoRNP Proteins Are Targets of Autoantibodies in Xenobiotic-Induced Autoimmunity. Clinical Immunology 2001, 101: 38-50. PMID: 11580225, DOI: 10.1006/clim.2001.5099.Peer-Reviewed Original ResearchConceptsSJL/J miceSmall nucleolar ribonucleoproteinSnoRNP proteinsCajal bodiesNucleolar ribonucleoproteinMetaphase chromosomesAmphibian cellsJ miceFibrillarinInterphase cellsProtein componentsNucleolar immunofluorescence patternProteinAnti-nucleolar antibodiesCell linesTarget of autoantibodiesAutoantibody responseAntibody responsePredominant targetImmunofluorescence patternMurineCellsRibonucleoproteinChromosomesMice
2000
The genes for small nucleolar RNAs in Trypanosoma brucei are organized in clusters and are transcribed as a polycistronic RNA
Dunbar D, Chen A, Wormsley S, Baserga S. The genes for small nucleolar RNAs in Trypanosoma brucei are organized in clusters and are transcribed as a polycistronic RNA. Nucleic Acids Research 2000, 28: 2855-2861. PMID: 10908346, PMCID: PMC102681, DOI: 10.1093/nar/28.15.2855.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacteriophage P1Base SequenceBlotting, SouthernChromosome MappingCloning, MolecularDNA Restriction EnzymesGenesMolecular Sequence DataMultigene FamilyPolymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, ProtozoanRNA, Small NucleolarTandem Repeat SequencesTranscription, GeneticTrypanosoma brucei bruceiFibrillarin-associated Box C/D Small Nucleolar RNAs inTrypanosoma brucei SEQUENCE CONSERVATION AND IMPLICATIONS FOR 2′-O-RIBOSE METHYLATION OF rRNA*
Dunbar D, Wormsley S, Lowe T, Baserga S. Fibrillarin-associated Box C/D Small Nucleolar RNAs inTrypanosoma brucei SEQUENCE CONSERVATION AND IMPLICATIONS FOR 2′-O-RIBOSE METHYLATION OF rRNA*. Journal Of Biological Chemistry 2000, 275: 14767-14776. PMID: 10747997, DOI: 10.1074/jbc.m001180200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceChromosomal Proteins, Non-HistoneCloning, MolecularConserved SequenceDNA PrimersMethylationMolecular Sequence DataPrecipitin TestsRabbitsRiboseRNA, ProtozoanRNA, RibosomalRNA, Small NuclearSequence Homology, Amino AcidSequence Homology, Nucleic AcidTrypanosoma brucei bruceiConceptsRibose methylationBox C/D snoRNAsMethylation guide snoRNAsSmall nucleolar RNAsAncient eukaryotesBrucei extractsComparative genomicsEarly eukaryotesGuide snoRNAsD snoRNAsFibrillarin proteinSequence conservationSnoRNA sequencesNucleolar RNAsNucleotides upstreamBox CBox DSnoRNAsT. bruceiCommon proteinsEukaryotesFibrillarinRNA 1MethylationRRNA
1999
Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins.
Lyman S, Gerace L, Baserga S. Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins. RNA 1999, 5: 1597-604. PMID: 10606270, PMCID: PMC1369881, DOI: 10.1017/s1355838299991288.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsCell NucleolusCloning, MolecularConserved SequenceHeLa CellsHumansMiceMolecular Sequence DataMolecular WeightNuclear ProteinsRatsRecombinant ProteinsRibonucleoproteinsRibonucleoproteins, Small NucleolarSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentSequence Homology, Amino Acid
1998
The U14 snoRNA is required for 2'-O-methylation of the pre-18S rRNA in Xenopus oocytes.
Dunbar D, Baserga S. The U14 snoRNA is required for 2'-O-methylation of the pre-18S rRNA in Xenopus oocytes. RNA 1998, 4: 195-204. PMID: 9570319, PMCID: PMC1369608.Peer-Reviewed Original Research
1997
Mpp10p, a U3 Small Nucleolar Ribonucleoprotein Component Required for Pre-18S rRNA Processing in Yeast
Dunbar D, Wormsley S, Agentis T, Baserga S. Mpp10p, a U3 Small Nucleolar Ribonucleoprotein Component Required for Pre-18S rRNA Processing in Yeast. Molecular And Cellular Biology 1997, 17: 5803-5812. PMID: 9315638, PMCID: PMC232428, DOI: 10.1128/mcb.17.10.5803.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesCloning, MolecularEscherichia coliGenes, FungalHumansMiceMolecular Sequence DataMolecular WeightPhosphoproteinsRecombinant Fusion ProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, RibosomalRNA, Ribosomal, 18SSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidSpores, FungalConceptsYeast proteinsU3 snoRNPProtein componentsU3 small nucleolar ribonucleoproteinNovel protein componentsPre-rRNA processingSmall nucleolar ribonucleoproteinS. cerevisiae cellsSpecific protein componentsPulse-chase analysisMitotic phosphorylationRibosome biogenesisEssential genesMpp10pYeast SaccharomycesNovel proteinU3 snoRNARRNA precursorNucleolar ribonucleoproteinSites A0Tetrad analysisHuman proteinsNull allelesCerevisiae cellsGenBank searchMpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors.
Baserga S, Agentis T, Wormsley S, Dunbar D, Lee S. Mpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors. Nucleic Acids Symposium Series 1997, 64-7. PMID: 9478208.Peer-Reviewed Original ResearchConceptsPre-rRNA processingNew protein componentsU3 snoRNPProtein componentsU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinEssential genesSlow growthConditional promoterU3 snoRNAMpp10pNucleolar ribonucleoproteinRRNA precursorProcessing eventsYeastSnoRNPsMpp10ProteinModel systemDegrees C. AnalysisC. AnalysisSnoRNAsMutantsRibonucleoproteinSaccharomyces
1996
The U18 snRNA is not essential for pre-rRNA processing in Xenopus laevis.
Dunbar D, Ware V, Baserga S. The U18 snRNA is not essential for pre-rRNA processing in Xenopus laevis. RNA 1996, 2: 324-33. PMID: 8634913, PMCID: PMC1369375.Peer-Reviewed Original Research
1992
Distinct molecular signals for nuclear import of the nucleolar snRNA, U3.
Baserga S, Gilmore-Hebert M, Yang X. Distinct molecular signals for nuclear import of the nucleolar snRNA, U3. Genes & Development 1992, 6: 1120-1130. PMID: 1592260, DOI: 10.1101/gad.6.6.1120.Peer-Reviewed Original ResearchConceptsCap trimethylationNuclear importU3 RNADistinct molecular signalsXenopus oocytesRegulation of importU3 geneCytoplasmic phaseNucleolar snRNAOptimal importU3 snRNAMolecular signalsSnRNAU3 snRNPNucleotidesTrimethylationCytoplasmAutoantigenic proteinsRNAArtificial constructsStemImportOocytesBiogenesisSnRNP