2015
A protein interaction map of the LSU processome
McCann KL, Charette JM, Vincent NG, Baserga SJ. A protein interaction map of the LSU processome. Genes & Development 2015, 29: 862-875. PMID: 25877921, PMCID: PMC4403261, DOI: 10.1101/gad.256370.114.Peer-Reviewed Original ResearchConceptsLarge ribosomal subunitBiogenesis factorsRibosome biogenesis factorsTwo-hybrid assayProtein interaction mapsProtein-protein interactionsSystems biology approachInteractome mapHub proteinsBiology approachProcessomeProtein pairsRibosomal subunitInteraction mapCoordinated processConcerted actionCurrent knowledgeEukaryotesNOP4SubcomplexSubunitsProteinFourfold increaseMaturationAssembly
2014
Discovering the pre‐60S ribosome biogenesis factor interactome (560.7)
McCann K, Charette J, Vincent N, Baserga S. Discovering the pre‐60S ribosome biogenesis factor interactome (560.7). The FASEB Journal 2014, 28 DOI: 10.1096/fasebj.28.1_supplement.560.7.Peer-Reviewed Original ResearchRibosome assemblyGlycerol gradient sedimentation analysisTwo-hybrid analysisHigh-throughput yeastHigh-confidence interactionsPotential regulatory mechanismsGradient sedimentation analysisRNA helicasesBiogenesis factorsSmaller subcomplexesMDa complexNucleolar proteinsRibosomal subunitRegulatory mechanismsDifferent proteinsSubcomplexHelicasesGlycerol gradientsIndependent validation experimentsInteractomeSedimentation analysisYeastCurrent knowledgeProteinAssembly
2011
The initial U3 snoRNA:pre‐rRNA base‐pairing interaction required for pre‐18S rRNA folding revealed by in vivo chemical probing
Baserga S, Dutca L, Gallagher J. The initial U3 snoRNA:pre‐rRNA base‐pairing interaction required for pre‐18S rRNA folding revealed by in vivo chemical probing. The FASEB Journal 2011, 25: 704.1-704.1. DOI: 10.1096/fasebj.25.1_supplement.704.1.Peer-Reviewed Original ResearchU3 snoRNABase-pairing interactionsSmall subunitRibosomal subunitU3 small nucleolar RNAPre-rRNA foldingVivo chemical probingSmall nucleolar RNAsSmall ribosomal subunitRRNA foldingEukaryotic nucleolusRibosomal proteinsU3 snoRNPCytoplasmic ribosomesNucleolar RNAsGenetic manipulationS. cerevisiaeChemical probingSnoRNAsVivo chemicalRRNAEssential processSubunitsFoldingNucleotide reactivity