2024
The cytidine deaminase APOBEC3A regulates nucleolar function to promote cell growth and ribosome biogenesis
McCool M, Bryant C, Abriola L, Surovtseva Y, Baserga S. The cytidine deaminase APOBEC3A regulates nucleolar function to promote cell growth and ribosome biogenesis. PLOS Biology 2024, 22: e3002718. PMID: 38976757, PMCID: PMC11257408, DOI: 10.1371/journal.pbio.3002718.Peer-Reviewed Original ResearchRibosome biogenesis factorsRibosome biogenesisBiogenesis factorsCell growthNucleolar functionRegulation of nucleolar functionHuman ribosome biogenesisProtein synthesisProduction of ribosomesFamily of proteinsSource of mutagenesisLevel of protein synthesisCytidine deaminase familyIncreased cell growthPromote cell growthPre-rRNAPotential direct rolePre-mRNATransient overexpressionRibosomeGenomic mutationsBiogenesisMCF10A cellsMaturation stepsAPOBEC3A
2022
Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing
McCool M, Buhagiar A, Bryant C, Ogawa L, Abriola L, Surovtseva Y, Baserga S. Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing. RNA 2022, 29: rna.079149.122. PMID: 36323459, PMCID: PMC9808572, DOI: 10.1261/rna.079149.122.Peer-Reviewed Original ResearchRRNA transcriptionRRNA processingRibosomal subunit biogenesisRNA polymerase IRibosome biosynthesisSubunit biogenesisRibosome biogenesisRibosome assemblyAssembly factorsTranscription controlBiogenesis factorsRRNA productionSteady-state levelsRNA transcriptionPolymerase IComplex membersHuman cellsProtein synthesisP53 stabilizationTranscriptionEssential processBiogenesisCell proliferationDual roleRegulatory details
2016
Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis
Robson A, Owens ND, Baserga SJ, Khokha MK, Griffin JN. Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis. BMC Developmental Biology 2016, 16: 38. PMID: 27784267, PMCID: PMC5081970, DOI: 10.1186/s12861-016-0138-5.Peer-Reviewed Original ResearchConceptsRibosome biogenesis factorsBiogenesis factorsVentral blood islandsHuman congenital diseasesCranial neural crestRNA-seq dataAntisense mRNA probesBlood islandsEmbryonic developmentDistinct tissuesNeural crestProtein productionDifferential expressionTranscript numbersMachinery componentsSpecific tissuesSpecific phenotypesRibosomopathiesSitu hybridizationMRNA probesRibosomesExpressionTissue localizationPhenotypeFactor expressionNop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA
Zhang J, McCann KL, Qiu C, Gonzalez LE, Baserga SJ, Hall TM. Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA. Nature Communications 2016, 7: 13085. PMID: 27725644, PMCID: PMC5062617, DOI: 10.1038/ncomms13085.Peer-Reviewed Original ResearchConceptsEukaryotic ribosome biogenesisCorrect subcellular locationRibosome assembly factorsPre-ribosomal RNAPumilio repeatsRibosome biogenesisHuman ribosomopathiesAssembly factorsBiogenesis factorsRepeat proteinsMature rRNASubcellular locationNop9RNA complexCleavage siteRRNATimely cleavageProteinStructural featuresFinal processing stepRibosomopathiesBiogenesisCleavageYeastNuclease
2015
A protein interaction map of the LSU processome
McCann KL, Charette JM, Vincent NG, Baserga SJ. A protein interaction map of the LSU processome. Genes & Development 2015, 29: 862-875. PMID: 25877921, PMCID: PMC4403261, DOI: 10.1101/gad.256370.114.Peer-Reviewed Original ResearchConceptsLarge ribosomal subunitBiogenesis factorsRibosome biogenesis factorsTwo-hybrid assayProtein interaction mapsProtein-protein interactionsSystems biology approachInteractome mapHub proteinsBiology approachProcessomeProtein pairsRibosomal subunitInteraction mapCoordinated processConcerted actionCurrent knowledgeEukaryotesNOP4SubcomplexSubunitsProteinFourfold increaseMaturationAssembly
2014
Discovering the pre‐60S ribosome biogenesis factor interactome (560.7)
McCann K, Charette J, Vincent N, Baserga S. Discovering the pre‐60S ribosome biogenesis factor interactome (560.7). The FASEB Journal 2014, 28 DOI: 10.1096/fasebj.28.1_supplement.560.7.Peer-Reviewed Original ResearchRibosome assemblyGlycerol gradient sedimentation analysisTwo-hybrid analysisHigh-throughput yeastHigh-confidence interactionsPotential regulatory mechanismsGradient sedimentation analysisRNA helicasesBiogenesis factorsSmaller subcomplexesMDa complexNucleolar proteinsRibosomal subunitRegulatory mechanismsDifferent proteinsSubcomplexHelicasesGlycerol gradientsIndependent validation experimentsInteractomeSedimentation analysisYeastCurrent knowledgeProteinAssembly
2013
NOL11, implicated in the pathogenesis of North American Indian Childhood Cirrhosis, is required for pre‐rRNA transcription and processing
Baserga S, Freed E, Prieto J, McCann K, McStay B. NOL11, implicated in the pathogenesis of North American Indian Childhood Cirrhosis, is required for pre‐rRNA transcription and processing. The FASEB Journal 2013, 27: 552.1-552.1. DOI: 10.1096/fasebj.27.1_supplement.552.1.Peer-Reviewed Original ResearchNorth American Indian childhood cirrhosisRibosome biogenesisSmall subunitC-terminusInteraction partnersDefective protein-protein interactionsTwo-hybrid cDNA libraryUncharacterized nucleolar proteinRibosome biogenesis factorsRibosome biogenesis defectsTwo-hybrid analysisPre-rRNA transcriptionRibosomal small subunitProtein-protein interactionsCo-immunoprecipitation experimentsRDNA transcriptionBiogenesis defectsBiogenesis factorsNucleolar proteinsNovel proteinCDNA libraryCirhinAffinity purificationDisease mutationsSiRNA knockdown