1998
Glial cells have heart: rH1 Na+ channel mRNA and protein in spinal cord astrocytes
Black JA, Dib‐Hajj S, Cohen S, Hinson AW, Waxman SG. Glial cells have heart: rH1 Na+ channel mRNA and protein in spinal cord astrocytes. Glia 1998, 23: 200-208. PMID: 9633805, DOI: 10.1002/(sici)1098-1136(199807)23:3<200::aid-glia3>3.0.co;2-8.Peer-Reviewed Original ResearchConceptsTTX-resistant currentStellate astrocytesSpinal cordCultured spinal cord astrocytesVoltage-sensitive sodium currentsChannel mRNASodium currentDorsal root ganglion neuronsSodium channel immunoreactivityRT-PCRSpinal cord astrocytesTTX-sensitive currentsIntact spinal cordSpinal cord culturesReverse transcription-polymerase chain reactionSodium channel mRNASodium channel expressionTranscription-polymerase chain reactionTTX-resistant channelsPolyclonal antibodiesChannel immunoreactivityP7 ratsGanglion neuronsPolymerase chain reactionChannel antibodies
1997
NaG: A sodium channel‐like mRNA shared by Schwann cells and other neural crest derivatives
Felts PA, Black JA, Dib‐Hajj S, Waxman SG. NaG: A sodium channel‐like mRNA shared by Schwann cells and other neural crest derivatives. Glia 1997, 21: 269-276. PMID: 9383036, DOI: 10.1002/(sici)1098-1136(199711)21:3<269::aid-glia2>3.0.co;2-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGanglia, SpinalIn Situ HybridizationNeural CrestPolymerase Chain ReactionRatsRats, Sprague-DawleyRNA, MessengerSchwann CellsSodium ChannelsConceptsNeural crest originSchwann cellsGanglion neuronsNeural crest derivativesRat dorsal root ganglion neuronsAdrenal medulla chromaffin cellsDorsal root ganglion neuronsSuperior cervical ganglion neuronsSodium channelsCrest derivativesNeural crestNeuronal cell typesAfferent neuronsAfferent functionSitu hybridizationSensory neuronsNeural elementsSensory functionChromaffin cellsNAG mRNANeuronsRT-PCRNAGCell typesPresent studyTTX-Sensitive and -Resistant Na+ Currents, and mRNA for the TTX-Resistant rH1 Channel, Are Expressed in B104 Neuroblastoma Cells
Gu X, Dib-Hajj S, Rizzo M, Waxman S. TTX-Sensitive and -Resistant Na+ Currents, and mRNA for the TTX-Resistant rH1 Channel, Are Expressed in B104 Neuroblastoma Cells. Journal Of Neurophysiology 1997, 77: 236-246. PMID: 9120565, DOI: 10.1152/jn.1997.77.1.236.Peer-Reviewed Original ResearchConceptsB104 neuroblastoma cellsTTX-resistant channelsB104 cellsNeuroblastoma cellsWhole-cell patch-clamp methodAbsence of TTXTTX-resistant currentTTX-sensitive currentsPresence of TTXPA/pFTranscription-polymerase chain reactionLong QT syndromeCell linesSteady-state inactivationNeuroblastoma cell linesAlpha-subunit mRNAPatch-clamp methodTTX-sensitiveHalf-maximal inhibitionInactivation time constantsChannel mRNATTXMembrane excitabilitySubunit mRNAsRT-PCR