2009
The ataxia3 Mutation in the N-Terminal Cytoplasmic Domain of Sodium Channel Nav1.6 Disrupts Intracellular Trafficking
Sharkey LM, Cheng X, Drews V, Buchner DA, Jones JM, Justice MJ, Waxman SG, Dib-Hajj SD, Meisler MH. The ataxia3 Mutation in the N-Terminal Cytoplasmic Domain of Sodium Channel Nav1.6 Disrupts Intracellular Trafficking. Journal Of Neuroscience 2009, 29: 2733-2741. PMID: 19261867, PMCID: PMC2679640, DOI: 10.1523/jneurosci.6026-08.2009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCell LineChromosome MappingCytoplasmData Interpretation, StatisticalDNA, ComplementaryElectrophysiologyEthylnitrosoureaImmunohistochemistryMachado-Joseph DiseaseMiceMice, Inbred C57BLMutagensMutationMutation, MissenseNAV1.6 Voltage-Gated Sodium ChannelNerve Tissue ProteinsPatch-Clamp TechniquesSciatic NerveSodium ChannelsSubcellular FractionsTransfectionConceptsMutant channelsCytoplasmic N-terminal regionN-terminal cytoplasmic domainCytoplasmic N-terminal domainMouse chromosome 15N-terminal domainN-terminal regionAmino acid substitution p.Primary cerebellar granule cellsVoltage-dependent inward sodium currentMutant proteinsCytoplasmic domainJuvenile lethalityCis-GolgiTrafficking defectsPlasma membraneSodium channelsIntracellular traffickingProtein abundanceWild typeN-terminusGolgi complexMutant transcriptsChromosome 15Whole-cell patch-clamp studies
2003
Primary cortical motor neurons undergo apoptosis after axotomizing spinal cord injury
Hains B, Black J, Waxman S. Primary cortical motor neurons undergo apoptosis after axotomizing spinal cord injury. The Journal Of Comparative Neurology 2003, 462: 328-341. PMID: 12794736, DOI: 10.1002/cne.10733.Peer-Reviewed Original ResearchConceptsSpinal cord injuryCortical motor neuronsFluoro-GoldCorticospinal tractCord injuryMotor neuronsTerminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphateAdult male Sprague-Dawley ratsRetrograde tracer Fluoro-GoldMale Sprague-Dawley ratsApoptotic cell deathDorsal corticospinal tractPrimary motor cortexTracer Fluoro-GoldSprague-Dawley ratsVoluntary motor controlTUNEL-positive cellsCell deathEvidence of apoptosisSham surgeryDorsal funiculusMotor cortexPyramidal cellsLesion sitePositive cells
2002
Axotomy does not up-regulate expression of sodium channel Nav1.8 in Purkinje cells
Black J, Dusart I, Sotelo C, Waxman S. Axotomy does not up-regulate expression of sodium channel Nav1.8 in Purkinje cells. Brain Research 2002, 101: 126-131. PMID: 12007840, DOI: 10.1016/s0169-328x(02)00200-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsAxotomyCerebellumDisease Models, AnimalFemaleGanglia, SpinalGene Expression RegulationImmunohistochemistryMultiple SclerosisNAV1.8 Voltage-Gated Sodium ChannelNeurons, AfferentNeuropeptidesPurkinje CellsRatsRats, WistarRNA, MessengerSodium ChannelsUp-RegulationZebrafish ProteinsConceptsMultiple sclerosisPurkinje cellsSensory neuron-specific sodium channelsDorsal root ganglion neuronsAberrant expressionSodium channelsHuman multiple sclerosisPrimary sensory neuronsSodium channel Nav1.8Specific sodium channelsCerebellar Purkinje cellsGanglion neuronsSensory neuronsAxotomySurgical modelSodium channel transcriptsExperimental modelCerebellar functionChannel transcriptsNeuronsSitu hybridizationCellsExpressionNav1.8Sclerosis
2001
Direct Interaction with Contactin Targets Voltage-gated Sodium Channel Nav1.9/NaN to the Cell Membrane*
Liu C, Dib-Hajj S, Black J, Greenwood J, Lian Z, Waxman S. Direct Interaction with Contactin Targets Voltage-gated Sodium Channel Nav1.9/NaN to the Cell Membrane*. Journal Of Biological Chemistry 2001, 276: 46553-46561. PMID: 11581273, DOI: 10.1074/jbc.m108699200.Peer-Reviewed Original ResearchConceptsDorsal root gangliaRoot gangliaSodium channelsSmall sensory neuronsVoltage-gated sodium channelsTrigeminal ganglionNerve endingsC-fibersSensory neuronsNeuron somataChinese hamster ovary cell lineDifferent physiological propertiesGangliaHamster ovary cell lineNeuronal membranesChinese hamster ovary cellsOvary cell lineProtein complexesSurface expressionHamster ovary cellsCell linesAxonsSurface localizationCell membraneOvary cells
2000
Sensory neuron-specific sodium channel SNS is abnormally expressed in the brains of mice with experimental allergic encephalomyelitis and humans with multiple sclerosis
Black J, Dib-Hajj S, Baker D, Newcombe J, Cuzner M, Waxman S. Sensory neuron-specific sodium channel SNS is abnormally expressed in the brains of mice with experimental allergic encephalomyelitis and humans with multiple sclerosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 11598-11602. PMID: 11027357, PMCID: PMC17246, DOI: 10.1073/pnas.97.21.11598.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainEncephalomyelitis, Autoimmune, ExperimentalHumansImmunohistochemistryMiceMultiple SclerosisNeurons, AfferentSodium ChannelsConceptsExperimental allergic encephalomyelitisMultiple sclerosisAllergic encephalomyelitisClinical abnormalitiesChannel expressionPurkinje cellsTrigeminal ganglion neuronsBrains of micePeripheral nervous systemSodium channel expressionIon channel expressionCerebellar Purkinje cellsAbnormal repertoiresAxonal degenerationControl miceGanglion neuronsControl subjectsMouse modelNormal brainAnimal modelsNervous systemNeurological diseasesSodium channelsProtein expressionAbnormal patternsLocalization of the tetrodotoxin-resistant sodium channel NaN in nociceptors
Fjell J, Hjelmström P, Hormuzdiar W, Milenkovic M, Aglieco F, Tyrrell L, Dib-Hajj S, Waxman S, Black J. Localization of the tetrodotoxin-resistant sodium channel NaN in nociceptors. Neuroreport 2000, 11: 199-202. PMID: 10683857, DOI: 10.1097/00001756-200001170-00039.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAxonsCorneaFemaleGanglia, SpinalImage Processing, Computer-AssistedImmunohistochemistryMolecular Sequence DataMyelin SheathNAV1.9 Voltage-Gated Sodium ChannelNerve FibersNeurons, AfferentNeuropeptidesNociceptorsPresynaptic TerminalsRanvier's NodesRatsRats, Sprague-DawleySciatic NerveSodium ChannelsTetrodotoxinConceptsSciatic nerveSmall diameter primary sensory neuronsSodium currentTetrodotoxin-resistant sodium channelsTetrodotoxin-resistant sodium currentDorsal root ganglion neuronsSodium channelsPrimary sensory neuronsAxonal sodium currentsNodes of RanvierNociceptive transmissionChannel immunoreactivityGanglion neuronsUnmyelinated fibersAxon terminalsSensory neuronsNerveImmunoreactivityAxonsNeuronsSpecific peptidesNociceptorsIB4CorneaAntibodies
1999
Changes in expression of voltage‐gated potassium channels in dorsal root ganglion neurons following axotomy
Ishikawa K, Tanaka M, Black J, Waxman S. Changes in expression of voltage‐gated potassium channels in dorsal root ganglion neurons following axotomy. Muscle & Nerve 1999, 22: 502-507. PMID: 10204786, DOI: 10.1002/(sici)1097-4598(199904)22:4<502::aid-mus12>3.0.co;2-k.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxotomyGanglia, SpinalImmunohistochemistryIon Channel GatingMaleMembrane PotentialsNeuronsPotassium ChannelsRatsRats, Sprague-DawleyConceptsDorsal root ganglion neuronsDRG neuronsVoltage-gated potassium channelsAxonal injuryGanglion neuronsPotassium channelsChannel expressionNormal DRG neuronsChronic pain syndromeSodium channel expressionSpectrum of subtypesVoltage-gated sodium channelsSpecific potassium channelsPain syndromeDRG cellsAdult ratsNervous systemAxotomyKv expressionNeuronsImmunocytochemical methodsMolecular correlatesElectrical excitabilitySodium channelsImmunoreactivity
1998
Mechanisms of enhancement of neurite regeneration in vitro following a conditioning sciatic nerve lesion
Lankford K, Waxman S, Kocsis J. Mechanisms of enhancement of neurite regeneration in vitro following a conditioning sciatic nerve lesion. The Journal Of Comparative Neurology 1998, 391: 11-29. PMID: 9527536, PMCID: PMC2605358, DOI: 10.1002/(sici)1096-9861(19980202)391:1<11::aid-cne2>3.0.co;2-u.Peer-Reviewed Original ResearchConceptsDorsal root gangliaConditioning lesionNerve injuryNerve regenerationAffected dorsal root ganglionControl dorsal root gangliaDenervated peripheral nervePrior nerve injurySciatic nerve lesionCultured DRG neuronsSciatic nerve transectionPeripheral target tissuesPeripheral nerve stumpRapid nerve regenerationAbility of neuronsSecond axotomyNerve lesionsDRG neuronsNerve transectionNerve stumpRoot gangliaControl neuronsPeripheral nervesNerve tractsAdult rats
1997
Immunolocalization of the Na+–Ca2+ exchanger in mammalian myelinated axons
Steffensen I, Waxman S, Mills L, Stys P. Immunolocalization of the Na+–Ca2+ exchanger in mammalian myelinated axons. Brain Research 1997, 776: 1-9. PMID: 9439790, DOI: 10.1016/s0006-8993(97)00868-8.Peer-Reviewed Original ResearchConceptsOptic nerveSpinal cordDorsal root axonsSciatic nerve sectionRat optic nerveCentral myelinated axonsCardiac type IFiner processesSimilar staining patternNerve sectionDorsal columnsSciatic nerveFrozen cryostat sectionsAnoxic injuryAxonal profilesImmunofluorescence labeling techniqueMyelinated axonsCell bodiesCryostat sectionsImportant mediatorAxonal localizationMammalian axonsNerveAxonsStaining patternNGF has opposing effects on Na+ channel III and SNS gene expression in spinal sensory neurons
Black J, Langworthy K, Hinson A, Dib-Hajj S, Waxman S. NGF has opposing effects on Na+ channel III and SNS gene expression in spinal sensory neurons. Neuroreport 1997, 8: 2331-2335. PMID: 9243635, DOI: 10.1097/00001756-199707070-00046.Peer-Reviewed Original ResearchConceptsNeurotrophin nerve growth factorSmall DRG neuronsExogenous NGFDRG neuronsDifferent sodium channel genesSmall dorsal root ganglion neuronsDorsal root ganglion neuronsSciatic nerve transectionMRNA levelsSpinal sensory neuronsNerve growth factorNerve transectionDRG culturesGanglion neuronsAlpha-IIIAdult ratsSensory neuronsPeripheral targetsSodium channel transcriptsMRNA expressionMembrane excitabilityNeuronsGrowth factorRetrograde transportChannel transcripts
1996
Manipulation of the delayed rectifier Kv1.5 potassium channel in glial cells by antisense oligodeoxynucleotides
Roy M, Saal D, Perney T, Sontheimer H, Waxman S, Kaczmarek L. Manipulation of the delayed rectifier Kv1.5 potassium channel in glial cells by antisense oligodeoxynucleotides. Glia 1996, 18: 177-184. PMID: 8915650, DOI: 10.1002/(sici)1098-1136(199611)18:3<177::aid-glia2>3.0.co;2-x.Peer-Reviewed Original ResearchConceptsGlial cellsKv1.5 channel proteinSpinal cordKv1.5 proteinCultured spinal cordTEA-insensitive currentSpinal cord astrocytesRectifier current densityPotassium channel typesAntisense oligodeoxynucleotide treatmentKv1.5 potassium channelAdult ratsCerebellar slicesChannel proteinsAstrocytesOligodeoxynucleotide treatmentPotassium channelsRectifier currentEndfoot processesSuch treatmentCurrent activationAntisense oligodeoxynucleotidesCordCellsTreatment
1995
Differential up-regulation of sodium channel α- and β1-subunit mRNAs in cultured embryonic DRG neurons following exposure to NGF
Zur K, Oh Y, Waxman S, Black J. Differential up-regulation of sodium channel α- and β1-subunit mRNAs in cultured embryonic DRG neurons following exposure to NGF. Brain Research 1995, 30: 97-105. PMID: 7609649, DOI: 10.1016/0169-328x(94)00283-k.Peer-Reviewed Original ResearchConceptsSodium channel alphaNerve growth factorChannel alphaDRG neuronsCultured embryonic DRG neuronsΒ1 subunit mRNAPattern of expressionDorsal root ganglion neuronsSodium channel subunitsEmbryonic DRG neuronsPresence of NGFSitu hybridization cytochemistryAbsence of NGFBeta 1 mRNABeta-subunit mRNANAG mRNAChannel subunitsDevelopment proceedsSodium channel αE16 embryosGanglion neuronsMRNAAlpha-IIIChannel αAlpha IIDifferential Na+ channel β1 subunit mRNA expression in stellate and flat astrocytes cultured from rat cortex and cerebellum: A combined in situ hybridization and immunocytochemistry study
Oh Y, Waxman S. Differential Na+ channel β1 subunit mRNA expression in stellate and flat astrocytes cultured from rat cortex and cerebellum: A combined in situ hybridization and immunocytochemistry study. Glia 1995, 13: 166-173. PMID: 7782102, DOI: 10.1002/glia.440130303.Peer-Reviewed Original ResearchConceptsGFAP-positive astrocytesRat brainChannel beta 1 subunit (Na beta 1) mRNAMRNA expressionBeta 1 mRNA expressionSubunit mRNA expressionBeta 1 mRNABeta 1 subunit mRNARat cortexGranule cellsStellate astrocytesRat astrocytesAstrocytesChannel mRNAImmunocytochemistry methodSubunit mRNAsImmunocytochemistry studiesCerebellumBrainMRNASitu hybridizationRecent studiesDifferential expressionExpressionCells
1994
Type II sodium channels in spinal cord astrocytes in situ: Immunocytochemical observations
Black J, Westenbroek R, Ransom B, Catterall W, Waxman S. Type II sodium channels in spinal cord astrocytes in situ: Immunocytochemical observations. Glia 1994, 12: 219-227. PMID: 7851989, DOI: 10.1002/glia.440120307.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAstrocytesFemaleImmunohistochemistryIsomerismOptic NerveRatsRats, Sprague-DawleySodium ChannelsSpinal CordConceptsAdult rat spinal cordRat spinal cordOptic nerveSubtype-specific sequencesSpinal cordVentral funiculusSpinal cord white matter tractsSpinal cord white matterSodium channelsSpinal cord astrocytesCord white matterWhite matter tractsType ISodium channel alphaWhite matterAstrocytesNerveImmunocytochemical methodsCordChannel alphaSodium channel IIIsoform expressionDetectable labelingType II sodium channelsImmunocytochemical observations