1999
Plasticity of sodium channel expression in DRG neurons in the chronic constriction injury model of neuropathic pain
Dib-Hajj S, Fjell J, Cummins TR, Zheng Z, Fried K, LaMotte R, Black JA, Waxman S. Plasticity of sodium channel expression in DRG neurons in the chronic constriction injury model of neuropathic pain. Pain 1999, 83: 591-600. PMID: 10568868, DOI: 10.1016/s0304-3959(99)00169-4.Peer-Reviewed Original ResearchConceptsTTX-R sodium channelsChronic constriction injury modelDRG neuronsSodium currentSodium channelsNeuropathic painInjury modelAxotomized dorsal root ganglion (DRG) neuronsSmall-diameter DRG neuronsTTX-R sodium currentsDorsal root ganglion neuronsTTX-S currentsSodium channel expressionGanglion neuronsSciatic nerveChannel expressionSodium channel transcriptsNeuronsNa currentPainChannel transcriptsSignificant changesLevels of transcriptsHyperalgesiaPrevious studiesIn Vivo NGF Deprivation Reduces SNS Expression and TTX-R Sodium Currents in IB4-Negative DRG Neurons
Fjell J, Cummins T, Fried K, Black J, Waxman S. In Vivo NGF Deprivation Reduces SNS Expression and TTX-R Sodium Currents in IB4-Negative DRG Neurons. Journal Of Neurophysiology 1999, 81: 803-810. PMID: 10036280, DOI: 10.1152/jn.1999.81.2.803.Peer-Reviewed Original ResearchConceptsTTX-R sodium currentsNerve growth factorPA/pFDRG neuronsHigh antibody titersSodium current densityNGF-deprived neuronsSodium currentAntibody titersAdult ratsSmall dorsal root ganglion neuronsTetrodotoxin-resistant sodium channelsDorsal root ganglion neuronsTTX-R currentsSodium channel expressionMRNA hybridization signalsPathological painThermal hypoalgesiaGanglion neuronsControl neuronsIsolectin IB4Channel expressionNGF deprivationMRNA expressionNeurons
1998
SNS Na+ channel expression increases in dorsal root ganglion neurons in the carrageenan inflammatory pain model
Tanaka M, Cummins T, Ishikawa K, Dib-Hajj S, Black J, Waxman S. SNS Na+ channel expression increases in dorsal root ganglion neurons in the carrageenan inflammatory pain model. Neuroreport 1998, 9: 967-972. PMID: 9601651, DOI: 10.1097/00001756-199804200-00003.Peer-Reviewed Original ResearchConceptsSmall DRG neuronsDorsal root ganglion neuronsInjection of carrageenanDRG neuronsInflamed limbGanglion neuronsSodium currentTTX-R sodium currentsTetrodotoxin-resistant sodium currentInflammatory pain modelDevelopment of hyperexcitabilitySodium channel expressionPatch-clamp recordingsInflammatory painPain modelChronic painCarrageenan injectionNociceptive cellsContralateral sideNaive ratsChannel expressionProjection fieldsMRNA expressionNeuronsSodium channels
1996
Expression of mRNA for a sodium channel in subfamily 2 in spinal sensory neurons
Waxman S, Black J. Expression of mRNA for a sodium channel in subfamily 2 in spinal sensory neurons. Neurochemical Research 1996, 21: 395-401. PMID: 8734431, DOI: 10.1007/bf02527702.Peer-Reviewed Original ResearchConceptsDorsal root gangliaSpinal sensory neuronsSchwann cellsDRG neuronsSensory neuronsRat dorsal root gangliaSodium channelsΒ1 subunitExpression of mRNARoot gangliaSpinal cordSitu hybridization cytochemistryNeuronsΑ-subunitAntisense riboprobesBlot analysisType IMRNACellsExpressionHigh levelsGangliaRNA blot analysisHippocampusCord
1995
Na+ channel β1 subunit mRNA expression in developing rat central nervous system
Sashihara S, Oh Y, Black J, Waxman S. Na+ channel β1 subunit mRNA expression in developing rat central nervous system. Brain Research 1995, 34: 239-250. PMID: 8750827, DOI: 10.1016/0169-328x(95)00168-r.Peer-Reviewed Original ResearchConceptsGranule cell layerCell layerRat central nervous systemBeta 1Channel beta 1 subunitRexed's lamina IXDentate granule cellsPyramidal cell layerPostnatal day 2Beta 1 expressionSpecific neuronal populationsVentral-dorsal gradientCentral nervous systemSubunit mRNA expressionVoltage-dependent sodium channelsExternal granule cell layerInternal granule cell layerDorsal hornVentral hornLamina IXSpinal cordBeta 1 subunitAdult ratsIntensity of labelingGranule cellsAn orphan nuclear receptor, mROR α, and its spatial expression in adult mouse brain
Matsui T, Sashihara S, Oh Y, Waxman S. An orphan nuclear receptor, mROR α, and its spatial expression in adult mouse brain. Brain Research 1995, 33: 217-226. PMID: 8750880, DOI: 10.1016/0169-328x(95)00126-d.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesBlotting, NorthernBrainCell DifferentiationCell LineGene ExpressionHumansIn Situ HybridizationMiceMolecular Sequence DataNeuronsNuclear Receptor Subfamily 1, Group F, Member 1Olfactory BulbOrgan SpecificityReceptors, Cytoplasmic and NuclearReceptors, Retinoic AcidSequence Homology, Amino AcidThalamusTrans-ActivatorsTransfectionTumor Cells, CulturedConceptsResponsive elementActivation of transcriptionThyroid hormone responsive elementRetinoic acid responsive elementNeural cell lineagesAcid-responsive elementLaminin B1 geneOrphan nuclear receptorHormone-responsive elementsRAR beta geneSitu hybridization analysisRetinoic acid receptor-related orphan receptorTranscription factorsAdult mouse brainCotransfection experimentsP19 cellsCell lineagesReceptor-related orphan receptorNorthern hybridizationAcid receptor-related orphan receptorHybridization analysisSpatial expressionOrphan receptorBeta geneNuclear receptorsExpression of sodium channel α- and β-subunits in the nervous system of themyelin-deficient rat
Felts P, Black J, Waxman S. Expression of sodium channel α- and β-subunits in the nervous system of themyelin-deficient rat. Brain Cell Biology 1995, 24: 654-666. PMID: 7500121, DOI: 10.1007/bf01179816.Peer-Reviewed Original ResearchConceptsSodium channel αMale littermatesSubtype-specific riboprobesDorsal root gangliaChannel αNormal male littermatesSodium channel subunitsUnaffected male littermatesDays of ageAdult expression patternRoot gangliaSpinal cordExpression patternsAdult ratsNervous systemUnaffected littermatesSodium channelsLittermatesPattern of expressionChannel subunitsRatsGreater expressionSitu hybridization techniqueUnaffected animalsPrevious reportsNa+ channel β1 subunit mRNA: differential expression in rat spinal sensory neurons
Oh Y, Sashihara S, Black J, Waxman S. Na+ channel β1 subunit mRNA: differential expression in rat spinal sensory neurons. Brain Research 1995, 30: 357-361. PMID: 7637585, DOI: 10.1016/0169-328x(95)00052-t.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGanglia, SpinalGene ExpressionIn Situ HybridizationNeurons, AfferentRatsRNA, MessengerSodium ChannelsSpinal CordConceptsDRG neuronsNervous systemChannel beta 1 subunit (Na beta 1) mRNARat dorsal root ganglion neuronsCell bodiesDorsal root ganglion neuronsSubunit mRNAsBeta 1 mRNA expressionRat central nervous systemSmall DRG neuronsLarge DRG neuronsSpinal sensory neuronsPeripheral nervous systemPostnatal day 4Central nervous systemBeta 1 mRNABeta 1 subunit mRNASitu hybridization histochemistryAdult DRGGanglion neuronsSensory neuronsDay 4Hybridization histochemistryMRNA expressionNeuronsDifferential up-regulation of sodium channel α- and β1-subunit mRNAs in cultured embryonic DRG neurons following exposure to NGF
Zur K, Oh Y, Waxman S, Black J. Differential up-regulation of sodium channel α- and β1-subunit mRNAs in cultured embryonic DRG neurons following exposure to NGF. Brain Research 1995, 30: 97-105. PMID: 7609649, DOI: 10.1016/0169-328x(94)00283-k.Peer-Reviewed Original ResearchConceptsSodium channel alphaNerve growth factorChannel alphaDRG neuronsCultured embryonic DRG neuronsΒ1 subunit mRNAPattern of expressionDorsal root ganglion neuronsSodium channel subunitsEmbryonic DRG neuronsPresence of NGFSitu hybridization cytochemistryAbsence of NGFBeta 1 mRNABeta-subunit mRNANAG mRNAChannel subunitsDevelopment proceedsSodium channel αE16 embryosGanglion neuronsMRNAAlpha-IIIChannel αAlpha IIDifferential Na+ channel β1 subunit mRNA expression in stellate and flat astrocytes cultured from rat cortex and cerebellum: A combined in situ hybridization and immunocytochemistry study
Oh Y, Waxman S. Differential Na+ channel β1 subunit mRNA expression in stellate and flat astrocytes cultured from rat cortex and cerebellum: A combined in situ hybridization and immunocytochemistry study. Glia 1995, 13: 166-173. PMID: 7782102, DOI: 10.1002/glia.440130303.Peer-Reviewed Original ResearchConceptsGFAP-positive astrocytesRat brainChannel beta 1 subunit (Na beta 1) mRNAMRNA expressionBeta 1 mRNA expressionSubunit mRNA expressionBeta 1 mRNABeta 1 subunit mRNARat cortexGranule cellsStellate astrocytesRat astrocytesAstrocytesChannel mRNAImmunocytochemistry methodSubunit mRNAsImmunocytochemistry studiesCerebellumBrainMRNASitu hybridizationRecent studiesDifferential expressionExpressionCells
1994
Rat brain Na+ channel mRNAs in non‐excitable Schwann cells
Oh Y, Black J, Waxman S. Rat brain Na+ channel mRNAs in non‐excitable Schwann cells. FEBS Letters 1994, 350: 342-346. PMID: 8070590, DOI: 10.1016/0014-5793(94)00807-8.Peer-Reviewed Original ResearchIn situ hybridization localization of the Na+ channel β1 subunit mRNA in rat CNS neurons
Oh Y, Sashihara S, Waxman S. In situ hybridization localization of the Na+ channel β1 subunit mRNA in rat CNS neurons. Neuroscience Letters 1994, 176: 119-122. PMID: 7970226, DOI: 10.1016/0304-3940(94)90885-0.Peer-Reviewed Original ResearchConceptsBeta 1 mRNASpinal cordGranule cellsChannel beta 1 subunit (Na beta 1) mRNABeta 1 mRNA expressionDentate granule cellsAdult rat hippocampusCA1 pyramidal cellsRat CNS neuronsBeta 1 subunit mRNASitu hybridization histochemistryCNS neuronsPyramidal cellsRat hippocampusSitu hybridization localizationChannel β1Gray matterHybridization histochemistryPurkinje cellsMRNA expressionSubunit mRNAsModerate expressionDifferent neuronsStrong expressionNeurons