2011
Analysis of (R)- and (S)-[11C]rolipram Kinetics in Canine Myocardium for the Evaluation of Phosphodiesterase-4 with PET
Lortie M, DaSilva JN, Kenk M, Thorn S, Davis D, Birnie D, Beanlands RS, deKemp RA. Analysis of (R)- and (S)-[11C]rolipram Kinetics in Canine Myocardium for the Evaluation of Phosphodiesterase-4 with PET. Molecular Imaging And Biology 2011, 14: 225-236. PMID: 21424298, DOI: 10.1007/s11307-011-0482-6.Peer-Reviewed Original ResearchAnimalsCarbon RadioisotopesCyclic Nucleotide Phosphodiesterases, Type 4DogsModels, BiologicalMyocardiumPositron-Emission TomographyRolipramStereoisomerismTime Factors
2009
Reduced in vivo phosphodiesterase-4 response to acute noradrenaline challenge in diet-induced obese rats
Greene M, Thackeray JT, Kenk M, Thorn SL, Bevilacqua L, Harper ME, Beanlands RS, DaSilva JN. Reduced in vivo phosphodiesterase-4 response to acute noradrenaline challenge in diet-induced obese rats. Canadian Journal Of Physiology And Pharmacology 2009, 87: 196-202. PMID: 19295660, DOI: 10.1139/y09-001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalorimetry, IndirectCyclic Nucleotide Phosphodiesterases, Type 4DesipramineDietary FatsMaleNorepinephrineObesityRatsRats, Sprague-DawleyRolipramSignal TransductionSympathetic Nervous SystemConceptsDiet-induced obeseDR ratsDiet-induced obese ratsObese animal modelsSympathetic nervous activityPersistence of obesityHigh-fat dietPhosphodiesterase 4 inhibitorWeeks of feedingEnergy expenditure measuresDIO animalsNoradrenaline challengeDesipramine pretreatmentObese ratsCaloric intakeNoradrenaline stimulationNervous activityAnimal modelsPDE4 levelsNormal increaseWeight gainH pretreatmentSkeletal muscleRatsProgressive alteration
2006
In vivo selective binding of (R)-[11C]rolipram to phosphodiesterase-4 provides the basis for studying intracellular cAMP signaling in the myocardium and other peripheral tissues
Kenk M, Greene M, Thackeray J, deKemp RA, Lortie M, Thorn S, Beanlands RS, DaSilva JN. In vivo selective binding of (R)-[11C]rolipram to phosphodiesterase-4 provides the basis for studying intracellular cAMP signaling in the myocardium and other peripheral tissues. Nuclear Medicine And Biology 2006, 34: 71-77. PMID: 17210463, DOI: 10.1016/j.nucmedbio.2006.10.002.Peer-Reviewed Original ResearchMeSH Keywords3',5'-Cyclic-AMP PhosphodiesterasesAnimalsBrainCarbon RadioisotopesCyclic AMPCyclic Nucleotide Phosphodiesterases, Type 1Cyclic Nucleotide Phosphodiesterases, Type 4HeartMaleMetabolic Clearance RateMyocardiumOrgan SpecificityPhosphodiesterase InhibitorsProtein BindingRadionuclide ImagingRadiopharmaceuticalsRatsRats, Sprague-DawleyRolipramSensitivity and SpecificityTissue DistributionConceptsPhosphodiesterase 4BAY 60Ro 20Male Sprague-Dawley ratsIntracellular cAMPSprague-Dawley ratsNeurohormonal modulationPeripheral tissuesAutoradiography studiesAdipose tissuePDE4 levelsTracer uptakeVivo findingsCAMP-mediated signalingBiodistribution studiesPDE4 activityRolipramSkeletal muscleCAMP levelsTracer retentionCardiac regionCilostazolMyocardiumZaprinastTissue