2021
Chromatin tracing and multiplexed imaging of nucleome architectures (MINA) and RNAs in single mammalian cells and tissue
Liu M, Yang B, Hu M, Radda JSD, Chen Y, Jin S, Cheng Y, Wang S. Chromatin tracing and multiplexed imaging of nucleome architectures (MINA) and RNAs in single mammalian cells and tissue. Nature Protocols 2021, 16: 2667-2697. PMID: 33903756, PMCID: PMC9007104, DOI: 10.1038/s41596-021-00518-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChromatinGene Expression ProfilingHumansMolecular ImagingRNASingle-Cell AnalysisConceptsSame single cellNucleome architecturesGene expressionMammalian tissuesChromatin foldingNuclear laminaSingle cellsNumerous RNA speciesDifferent biological processesSingle mammalian cellsDifferent cell typesMultiplexed imagingGenomic organizationGenomic architectureChromatin loopsGenomic regionsRNA speciesIndividual chromosomesMammalian cellsGenomic techniquesBiological processesDetailed protocolCopy numberCell typesNormal development
2016
Spatial organization of chromatin domains and compartments in single chromosomes
Wang S, Su JH, Beliveau BJ, Bintu B, Moffitt JR, Wu CT, Zhuang X. Spatial organization of chromatin domains and compartments in single chromosomes. Science 2016, 353: 598-602. PMID: 27445307, PMCID: PMC4991974, DOI: 10.1126/science.aaf8084.Peer-Reviewed Original ResearchConceptsIndividual chromosomesChromatin domainsX chromosomeNumerous genomic regionsSpatial organizationInactive X chromosomeGenome functionChromatin organizationGenomic regionsFractal globule modelSingle chromosomePolarized mannerChromosomesDifferent foldingTADCompartmentsAutosomesChromatinDomainTADsFoldingRegulationCapture studies
2015
Spatially resolved, highly multiplexed RNA profiling in single cells
Chen KH, Boettiger AN, Moffitt JR, Wang S, Zhuang X. Spatially resolved, highly multiplexed RNA profiling in single cells. Science 2015, 348: aaa6090. PMID: 25858977, PMCID: PMC4662681, DOI: 10.1126/science.aaa6090.Peer-Reviewed Original ResearchConceptsRNA speciesMultiplexed error-robust fluorescenceSingle-molecule imaging approachesDistinct RNA speciesIndividual cellsGene regulatory networksPairs of genesSingle cellsDistinct spatial distribution patternsUnannotated genesSingle-molecule labelingRegulatory networksNovel functionRNA profilingExpression profilesCellular behaviorCopy numberSitu hybridizationRich repertoireSpatial distribution patternsGenesDistribution patternsSpeciesCellsSpatial landscape