2020
ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization
Hu M, Yang B, Cheng Y, Radda JSD, Chen Y, Liu M, Wang S. ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization. Scientific Reports 2020, 10: 22031. PMID: 33328483, PMCID: PMC7745008, DOI: 10.1038/s41598-020-76439-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA ProbesGenome, HumanHumansIn Situ Hybridization, FluorescenceMiceTime FactorsUser-Computer InterfaceConceptsSingle-molecule RNA FISHSpecific genomic lociSitu hybridizationNucleome architecturesRNA FISHGenomic lociRNA speciesMultiplexed fluorescenceFISH techniqueFishRecent technological advancesMultiplexed imagingHybridizationMultiplexed mannerLociSpeciesFluorescencePowerful methodNotable exampleProbeProbe designTechnological advancesLamina-Dependent Stretching and Unconventional Chromosome Compartments in Early C. elegans Embryos
Sawh AN, Shafer MER, Su JH, Zhuang X, Wang S, Mango SE. Lamina-Dependent Stretching and Unconventional Chromosome Compartments in Early C. elegans Embryos. Molecular Cell 2020, 78: 96-111.e6. PMID: 32105612, PMCID: PMC7263362, DOI: 10.1016/j.molcel.2020.02.006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansChromosomesEmbryo, NonmammalianGastrulationIn Situ Hybridization, FluorescenceMolecular ConformationNuclear LaminaConceptsB compartmentsEmbryonic chromosomesChromosome compartmentsInactive compartmentsChromosome domainsNuclear laminaEntire autosomesChromosomesCompartment boundariesUnappreciated roleDiverse conformationsDevelopmental contextChromosome stretchingCompartmentsConformationAutosomesChromatinGastrulationEmbryogenesisVariable positioningLaminaEmbryos
2015
Spatially resolved, highly multiplexed RNA profiling in single cells
Chen KH, Boettiger AN, Moffitt JR, Wang S, Zhuang X. Spatially resolved, highly multiplexed RNA profiling in single cells. Science 2015, 348: aaa6090. PMID: 25858977, PMCID: PMC4662681, DOI: 10.1126/science.aaa6090.Peer-Reviewed Original ResearchMeSH KeywordsFibroblastsGene Expression ProfilingHigh-Throughput Screening AssaysHumansIn Situ Hybridization, FluorescenceMolecular ImagingRNA ProbesRNA, MessengerSingle-Cell AnalysisTranscriptomeConceptsRNA speciesMultiplexed error-robust fluorescenceSingle-molecule imaging approachesDistinct RNA speciesIndividual cellsGene regulatory networksPairs of genesSingle cellsDistinct spatial distribution patternsUnannotated genesSingle-molecule labelingRegulatory networksNovel functionRNA profilingExpression profilesCellular behaviorCopy numberSitu hybridizationRich repertoireSpatial distribution patternsGenesDistribution patternsSpeciesCellsSpatial landscape