2016
Spatial organization shapes the turnover of a bacterial transcriptome
Moffitt JR, Pandey S, Boettiger AN, Wang S, Zhuang X. Spatial organization shapes the turnover of a bacterial transcriptome. ELife 2016, 5: e13065. PMID: 27198188, PMCID: PMC4874777, DOI: 10.7554/elife.13065.Peer-Reviewed Original ResearchConceptsBacterial transcriptomesSpatial organizationE. coli transcriptomeCo-translational insertionInner membrane proteinProtein mRNASignal recognition particlePost-transcriptional fatePost-transcriptional regulationSuper-resolution microscopyRNA degradosomePeriplasmic proteinsSignal peptideRNA sequencingTranscriptomeMembrane enrichmentSelective destabilizationMRNARNAProteinDegradosomeEukaryotesMembraneProkaryotesPowerful means
2013
Probing Allostery Through DNA
Kim S, Broströmer E, Xing D, Jin J, Chong S, Ge H, Wang S, Gu C, Yang L, Gao YQ, Su XD, Sun Y, Xie XS. Probing Allostery Through DNA. Science 2013, 339: 816-819. PMID: 23413354, PMCID: PMC3586787, DOI: 10.1126/science.1229223.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric RegulationBase SequenceBinding SitesDNA, B-FormDNA-Binding ProteinsDNA-Directed RNA PolymerasesEscherichia coliGene ExpressionGene Expression Regulation, BacterialLac RepressorsMolecular Dynamics SimulationNucleosomesProtein BindingProtein Structure, TertiaryReceptors, GlucocorticoidTranscription FactorsViral ProteinsCell Shape Can Mediate the Spatial Organization of the Bacterial Cytoskeleton
Wang S, Wingreen NS. Cell Shape Can Mediate the Spatial Organization of the Bacterial Cytoskeleton. Biophysical Journal 2013, 104: 541-552. PMID: 23442905, PMCID: PMC3566457, DOI: 10.1016/j.bpj.2012.12.027.Peer-Reviewed Original ResearchConceptsBacterial cytoskeletonCell shapeCytoskeletal filamentsBacterial cytoskeletal proteinsRod-shaped cellsCytoskeletal proteinsCell wallCytoskeletal polymerizationCytoskeletonSpatial patterningMreBConformational transitionSpatial organizationFilament lengthSame membraneFilamentsMembraneFtsZSpatial patternsChemical energyFilament bendingProteinPatterningProper controlMicrofluidic approach
2011
The bacterial actin MreB rotates, and rotation depends on cell-wall assembly
van Teeffelen S, Wang S, Furchtgott L, Huang KC, Wingreen NS, Shaevitz JW, Gitai Z. The bacterial actin MreB rotates, and rotation depends on cell-wall assembly. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 15822-15827. PMID: 21903929, PMCID: PMC3179079, DOI: 10.1073/pnas.1108999108.Peer-Reviewed Original ResearchConceptsCytoskeletal proteinsRod-like cell shapeCell wall synthesis machineryActin homolog MreBPeptidoglycan cell wallCell wall assemblyMultiple cytoskeletal proteinsMreB dynamicsBacterial morphogenesisCytoskeletal dynamicsSynthesis machineryCellular functionsCellular processesMotor proteinsCell shapeCell wallCytoskeletal motorsBacterial cellsMreBPersistent mannerProteinInsertion siteBiophysical simulationsBacteriaRod shape
2010
Actin-like cytoskeleton filaments contribute to cell mechanics in bacteria
Wang S, Arellano-Santoyo H, Combs PA, Shaevitz JW. Actin-like cytoskeleton filaments contribute to cell mechanics in bacteria. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 9182-9185. PMID: 20439764, PMCID: PMC2889055, DOI: 10.1073/pnas.0911517107.Peer-Reviewed Original ResearchConceptsCell wallLive Escherichia coli cellsActin homolog MreBBacterial cell shapePeptidoglycan cell wallEscherichia coli cellsBacterial cytoskeletonEukaryotic cellsCytoskeleton contributesFilamentous cytoskeletonCell shapeColi cellsCytoskeleton filamentsCytoskeletal filamentsCell mechanicsMreBCytoskeletonFirst evidenceProteinCellsBacteriaEukaryotesFilamentsEnzymeMechanical rigidity