Membrane Topography of the Hydrophobic Anchor Sequence of Poliovirus 3A and 3AB Proteins and the Functional Effect of 3A/3AB Membrane Association upon RNA Replication †
Fujita K, Krishnakumar SS, Franco D, Paul AV, London E, Wimmer E. Membrane Topography of the Hydrophobic Anchor Sequence of Poliovirus 3A and 3AB Proteins and the Functional Effect of 3A/3AB Membrane Association upon RNA Replication †. Biochemistry 2007, 46: 5185-5199. PMID: 17417822, PMCID: PMC2519882, DOI: 10.1021/bi6024758.Peer-Reviewed Original ResearchConceptsModel membrane vesiclesAnchor sequenceHydrophobic anchor sequenceMembrane vesiclesViral protein 3ABFull-length proteinN-terminal boundaryTransmembrane topographyMembrane associationSuppressor mutationsTransmembrane segmentsProtein 3ABRNA polymeraseMembranous vesiclesC-terminusMembrane topographyCytoplasmic surfaceHost cellsRNA replicationPoliovirus RNAHeLa cellsSingle tryptophanProteinUridylylation reactionLipid bilayers