2022
How to correct relative voxel scale factors for calculations of vector-difference Fourier maps in cryo-EM
Wang J, Liu J, Gisriel CJ, Wu S, Maschietto F, Flesher DA, Lolis E, Lisi GP, Brudvig GW, Xiong Y, Batista VS. How to correct relative voxel scale factors for calculations of vector-difference Fourier maps in cryo-EM. Journal Of Structural Biology 2022, 214: 107902. PMID: 36202310, PMCID: PMC10226527, DOI: 10.1016/j.jsb.2022.107902.Peer-Reviewed Original ResearchConceptsCryo-EM mapsAmino acid residuesAcid residuesCryo-electron microscopy mapIndividual amino acid residuesCyanobacteria Synechocystis spPCC 6803Synechocystis spMicroscopy mapsThermosynechococcus elongatusSARS-CoV-2 spike proteinLocal structural changesResiduesSpike proteinAtomic coordinatesElongatusSubunitsSpeciesProteinSpSimilar structureStructural changes
2019
Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome
Armache J, Gamarra N, Johnson S, Leonard J, Wu S, Narlikar G, Cheng Y. Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome. ELife 2019, 8: e46057. PMID: 31210637, PMCID: PMC6611695, DOI: 10.7554/elife.46057.Peer-Reviewed Original Research
2014
Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter
Kim J, Wu S, Tomasiak T, Mergel C, Winter M, Stiller S, Robles-Colmanares Y, Stroud R, Tampé R, Craik C, Cheng Y. Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter. Nature 2014, 517: 396-400. PMID: 25363761, PMCID: PMC4372080, DOI: 10.1038/nature13872.Peer-Reviewed Original Research
2013
A conformational switch in HP1 releases auto-inhibition to drive heterochromatin assembly
Canzio D, Liao M, Naber N, Pate E, Larson A, Wu S, Marina D, Garcia J, Madhani H, Cooke R, Schuck P, Cheng Y, Narlikar G. A conformational switch in HP1 releases auto-inhibition to drive heterochromatin assembly. Nature 2013, 496: 377-381. PMID: 23485968, PMCID: PMC3907283, DOI: 10.1038/nature12032.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsChromatin Assembly and DisassemblyChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneCryoelectron MicroscopyGene SilencingHeterochromatinHistonesMethylationModels, MolecularMolecular Sequence DataNucleosomesProtein Structure, TertiarySchizosaccharomycesSchizosaccharomyces pombe ProteinsXenopus laevis
2012
Electron Tomography of Paracrystalline 2D Arrays
Winkler H, Wu S, Taylor K. Electron Tomography of Paracrystalline 2D Arrays. Methods In Molecular Biology 2012, 955: 427-460. PMID: 23132074, PMCID: PMC7032944, DOI: 10.1007/978-1-62703-176-9_23.Peer-Reviewed Original ResearchAnimalsCryoelectron MicroscopyElectron Microscope TomographyImage Processing, Computer-AssistedInsectaMyosin Type VFabs Enable Single Particle cryoEM Studies of Small Proteins
Wu S, Avila-Sakar A, Kim J, Booth D, Greenberg C, Rossi A, Liao M, Li X, Alian A, Griner S, Juge N, Yu Y, Mergel C, Chaparro-Riggers J, Strop P, Tampé R, Edwards R, Stroud R, Craik C, Cheng Y. Fabs Enable Single Particle cryoEM Studies of Small Proteins. Structure 2012, 20: 582-592. PMID: 22483106, PMCID: PMC3322386, DOI: 10.1016/j.str.2012.02.017.Peer-Reviewed Original ResearchCryoelectron MicroscopyEscherichia coliEscherichia coli ProteinsHumansImage Processing, Computer-AssistedImmunoglobulin Fab FragmentsModels, MolecularMolecular WeightPeptide LibraryProprotein Convertase 9Proprotein ConvertasesProtein ConformationRecombinant ProteinsSerine EndopeptidasesVesicular Glutamate Transport Proteins
2007
Imaging Actomyosin In Situ
Taylor K, Wu S, Reedy M, Reedy M. Imaging Actomyosin In Situ. Methods In Cell Biology 2007, 79: 321-368. PMID: 17327163, DOI: 10.1016/s0091-679x(06)79013-6.Peer-Reviewed Original ResearchActomyosinAnimalsCryoelectron MicroscopyFreeze SubstitutionHeteropteraInsect ProteinsModels, ChemicalMuscles