2022
How to correct relative voxel scale factors for calculations of vector-difference Fourier maps in cryo-EM
Wang J, Liu J, Gisriel CJ, Wu S, Maschietto F, Flesher DA, Lolis E, Lisi GP, Brudvig GW, Xiong Y, Batista VS. How to correct relative voxel scale factors for calculations of vector-difference Fourier maps in cryo-EM. Journal Of Structural Biology 2022, 214: 107902. PMID: 36202310, PMCID: PMC10226527, DOI: 10.1016/j.jsb.2022.107902.Peer-Reviewed Original ResearchConceptsCryo-EM mapsAmino acid residuesAcid residuesCryo-electron microscopy mapIndividual amino acid residuesCyanobacteria Synechocystis spPCC 6803Synechocystis spMicroscopy mapsThermosynechococcus elongatusSARS-CoV-2 spike proteinLocal structural changesResiduesSpike proteinAtomic coordinatesElongatusSubunitsSpeciesProteinSpSimilar structureStructural changes
2013
Mechanistic Analysis of HP1 heterochromatin assembly
Narlikar G, Canzio D, Liao M, Naber N, Pate E, Larson A, Wu S, Cooke R, Schuck P, Cheng Y. Mechanistic Analysis of HP1 heterochromatin assembly. The FASEB Journal 2013, 27: 456.3-456.3. DOI: 10.1096/fasebj.27.1_supplement.456.3.Peer-Reviewed Original ResearchHeterochromatin protein 1Form of heterochromatinAuto-inhibited statePost-translation modificationsHP1 proteinsHeterochromatin assemblyHeterochromatin proteinsChromatin complexesLysine 9Adjacent nucleosomesHistone H3Swi6Competent stateMolecular basisChromodomainChromatinEM structuresDiverse interactionsProtein 1NucleosomesBiochemical dataProteinMechanistic analysisTestable hypothesesAssemblyA conformational switch in HP1 releases auto-inhibition to drive heterochromatin assembly
Canzio D, Liao M, Naber N, Pate E, Larson A, Wu S, Marina D, Garcia J, Madhani H, Cooke R, Schuck P, Cheng Y, Narlikar G. A conformational switch in HP1 releases auto-inhibition to drive heterochromatin assembly. Nature 2013, 496: 377-381. PMID: 23485968, PMCID: PMC3907283, DOI: 10.1038/nature12032.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsChromatin Assembly and DisassemblyChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneCryoelectron MicroscopyGene SilencingHeterochromatinHistonesMethylationModels, MolecularMolecular Sequence DataNucleosomesProtein Structure, TertiarySchizosaccharomycesSchizosaccharomyces pombe ProteinsXenopus laevis