2024
Lysosomal TBK1 responds to amino acid availability to relieve Rab7-dependent mTORC1 inhibition
Talaia G, Bentley-DeSousa A, Ferguson S. Lysosomal TBK1 responds to amino acid availability to relieve Rab7-dependent mTORC1 inhibition. The EMBO Journal 2024, 43: 3948-3967. PMID: 39103493, PMCID: PMC11405869, DOI: 10.1038/s44318-024-00180-8.Peer-Reviewed Original ResearchTANK-binding kinase 1MTORC1 activityAmino acid-dependent mTORC1 activationOrganelle quality controlRegulate cell growthElevated amino acid levelsAmino acid levelsAssociated with amyotrophic lateral sclerosisIncreased mTORC1 activityCellular demandSerine 72Amino acid availabilityLysosomal homeostasisSignaling proteinsMacromolecule degradationSites of amino acidsCell growthLysosomal poolMTORC1 inhibitionKinase 1Lysosomal functionAmino acidsInnate immunityLysosomesAmyotrophic lateral sclerosis
2019
PQLC2 recruits the C9orf72 complex to lysosomes in response to cationic amino acid starvation
Amick J, Tharkeshwar AK, Talaia G, Ferguson SM. PQLC2 recruits the C9orf72 complex to lysosomes in response to cationic amino acid starvation. Journal Of Cell Biology 2019, 219: e201906076. PMID: 31851326, PMCID: PMC7039192, DOI: 10.1083/jcb.201906076.Peer-Reviewed Original ResearchConceptsC9orf72 complexAmino acidsNormal lysosome functionAmino acid-sensing mechanismAmino acid starvationMembrane of lysosomesRegulated recruitmentComplex recruitmentCationic amino acidsHeterotrimeric complexPQLC2Acid-sensing mechanismsBinding partnerC9orf72 proteinComplex abundanceLysosome functionNovel mechanismLysosomesWDR41New roleComplexesRecruitmentSMCR8AcidProtein
2000
Molecular Cloning of a Human, Hemicholinium-3-Sensitive Choline Transporter
Apparsundaram S, Ferguson S, George A, Blakely R. Molecular Cloning of a Human, Hemicholinium-3-Sensitive Choline Transporter. Biochemical And Biophysical Research Communications 2000, 276: 862-867. PMID: 11027560, DOI: 10.1006/bbrc.2000.3561.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesBiological TransportCarrier ProteinsCholineChromosomes, Human, Pair 2Cloning, MolecularCOS CellsHemicholinium 3HumansLigandsMembrane Transport ProteinsMolecular Sequence DataOocytesProtein BindingRadiation Hybrid MappingRNA, MessengerSequence Homology, Amino AcidTransfectionXenopus laevisConceptsRadiation hybrid mapping techniquesCOS-7 cells resultsTransporter gene familyGlucose transporter gene familyHuman Genome ProjectNorthern blot analysisChromosomal localizationGene familyMolecular cloningGenome ProjectKb transcriptSequence informationMembrane vesiclesPrimary structureDependent choline uptakeMembrane fractionAmino acidsCDNAIodide transporterCells resultsBlot analysisChromosome 2q12Biosynthesis of acetylcholineMechanistic analysisCHO-1