2022
Integrating mechanical signals into cellular identity
Carley E, King MC, Guo S. Integrating mechanical signals into cellular identity. Trends In Cell Biology 2022, 32: 669-680. PMID: 35337714, PMCID: PMC9288541, DOI: 10.1016/j.tcb.2022.02.006.Peer-Reviewed Original ResearchConceptsDistinct gene expression programsComplex cellular programsGene expression programsLineage-committed cellsPluripotent stem cellsMulticellular organismsExpression programsCellular identityCellular programsMechanical signalsCell typesStem cellsMechanical inputCellsBiochemical inputsFunction correlationGenomeCytoskeletonOrganismsNumber of studiesImportant determinantComplex axisIdentityLarge arrayVivo
2021
EpoR stimulates rapid cycling and larger red cells during mouse and human erythropoiesis
Hidalgo D, Bejder J, Pop R, Gellatly K, Hwang Y, Maxwell Scalf S, Eastman AE, Chen JJ, Zhu LJ, Heuberger JAAC, Guo S, Koury MJ, Nordsborg NB, Socolovsky M. EpoR stimulates rapid cycling and larger red cells during mouse and human erythropoiesis. Nature Communications 2021, 12: 7334. PMID: 34921133, PMCID: PMC8683474, DOI: 10.1038/s41467-021-27562-4.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntigens, CDBcl-X ProteinCD4 AntigensCell CycleCell DifferentiationCell NucleusCell SizeCell SurvivalCyclin-Dependent Kinase Inhibitor p27Embryo, MammalianErythroblastsErythrocytesErythropoiesisErythropoietinFemaleFetusHealthy VolunteersHumansIronLiverMaleMice, Inbred C57BLModels, BiologicalProtein Serine-Threonine KinasesReceptors, ErythropoietinReceptors, TransferrinReticulocytesSignal TransductionConceptsCell size regulationCell sizeSequential cell divisionsEpoR functionErythroblast survivalMouse erythroblastsCell divisionSize regulationHuman erythropoiesisErythropoietin receptorCell cycleEpoRHypoxic stressRed cell sizeHigh erythropoietinLarger red cellsWild-type miceCyclingErythroblastsRegulationHigher EPO levelsMiceRed cellsSurvivalErythropoiesis
2019
MLL-AF9 initiates transformation from fast-proliferating myeloid progenitors
Chen X, Burkhardt DB, Hartman AA, Hu X, Eastman AE, Sun C, Wang X, Zhong M, Krishnaswamy S, Guo S. MLL-AF9 initiates transformation from fast-proliferating myeloid progenitors. Nature Communications 2019, 10: 5767. PMID: 31852898, PMCID: PMC6920141, DOI: 10.1038/s41467-019-13666-5.Peer-Reviewed Original ResearchAnimalsCell CycleCell DifferentiationCell ProliferationCell Transformation, NeoplasticCyclin D1Disease Models, AnimalFemaleGene Expression Regulation, LeukemicGene Knock-In TechniquesHumansKaplan-Meier EstimateLeukemia, Myeloid, AcuteMaleMice, TransgenicMyeloid Progenitor CellsMyeloid-Lymphoid Leukemia ProteinOncogene Proteins, FusionPiperazinesPrimary Cell CulturePrognosisPyridinesCell cycle dynamics in the reprogramming of cellular identity
Hu X, Eastman AE, Guo S. Cell cycle dynamics in the reprogramming of cellular identity. FEBS Letters 2019, 593: 2840-2852. PMID: 31562821, DOI: 10.1002/1873-3468.13625.Peer-Reviewed Original ResearchConceptsCell fate reprogrammingCell cycle dynamicsCellular identityDaughter cellsGenome replicationCell cycleSpecific cell cycle phasesCell fate regulationCell cycle controlRapid cell cyclesCell cycle phasesCycle dynamicsFate regulationEpigenomic changesCycle controlFate controlReprogrammingCell typesBiochemical processesReplicationComplex mechanismsCycle phaseGenomeCellsProminent exampleTargeting Fibrotic Signaling: A Review of Current Literature and Identification of Future Therapeutic Targets to Improve Wound Healing.
Hetzler PT, Dash BC, Guo S, Hsia HC. Targeting Fibrotic Signaling: A Review of Current Literature and Identification of Future Therapeutic Targets to Improve Wound Healing. Annals Of Plastic Surgery 2019, 83: e92-e95. PMID: 31246672, PMCID: PMC6851445, DOI: 10.1097/sap.0000000000001955.Peer-Reviewed Original ResearchConceptsTherapeutic targetAberrant wound healing processAppropriate physiologic responseMorbid disease processSurvival of myofibroblastsWound healingFibrotic signaling pathwaysTranscription factor/serum response factor (MRTF/SRF) pathwayFuture therapeutic targetsSmooth muscle actinFuture translational researchCurrent literatureFibrotic signalingTherapeutic optionsFibrotic lesionsTissue injuryWound healing processDisease processPhysiologic responsesSerum response factor pathwayMuscle actinFactor pathwayExcessive responseFibrosisTranslational research
2018
Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to Pluripotency
Hernandez C, Wang Z, Ramazanov B, Tang Y, Mehta S, Dambrot C, Lee YW, Tessema K, Kumar I, Astudillo M, Neubert TA, Guo S, Ivanova NB. Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to Pluripotency. Cell Stem Cell 2018, 23: 396-411.e8. PMID: 30146411, PMCID: PMC6128737, DOI: 10.1016/j.stem.2018.08.001.Peer-Reviewed Original ResearchConceptsInduced pluripotent stem cellsDNA damage response pathwayAcquisition of pluripotencyDamage response pathwayDNA methylation patternsStem cellsEmbryonic stem cellsESC enhancersPluripotent stem cellsMyc factorsPluripotent stateSomatic genesChromatin decompactionMolecular machineryEpigenetic remodelingEfficient reprogrammingResponse pathwaysSomatic cellsMethylation patternsPluripotencyHuman cellsEpigenomeEnhancerCellsKey role
2016
A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
Cheng J, Roden CA, Pan W, Zhu S, Baccei A, Pan X, Jiang T, Kluger Y, Weissman SM, Guo S, Flavell RA, Ding Y, Lu J. A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions. Nature Communications 2016, 7: 11178. PMID: 27025950, PMCID: PMC4820989, DOI: 10.1038/ncomms11178.Peer-Reviewed Original ResearchAnimalsBacterial ProteinsCell LineChromosome MappingCloning, MolecularClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Associated Protein 9DNADNA Restriction EnzymesEndonucleasesGene LibraryGenomeHumansMiceMicroRNAsOligonucleotide Array Sequence AnalysisRNA, Guide, CRISPR-Cas SystemsUntranslated Regions
2013
An Extensive Network of TET2-Targeting MicroRNAs Regulates Malignant Hematopoiesis
Cheng J, Guo S, Chen S, Mastriano SJ, Liu C, D’Alessio A, Hysolli E, Guo Y, Yao H, Megyola CM, Li D, Liu J, Pan W, Roden CA, Zhou XL, Heydari K, Chen J, Park IH, Ding Y, Zhang Y, Lu J. An Extensive Network of TET2-Targeting MicroRNAs Regulates Malignant Hematopoiesis. Cell Reports 2013, 5: 471-481. PMID: 24120864, PMCID: PMC3834864, DOI: 10.1016/j.celrep.2013.08.050.Peer-Reviewed Original ResearchConceptsKey tumor suppressorMyeloid differentiation biasTET2 expressionTranslocation 2 (TET2) geneMolecular regulationDifferentiation biasHematopoietic malignanciesTen-ElevenMalignant hematopoiesisTumor suppressorHematopoietic expansionActivity screenMiR-7MiRNAsExpression of TET2Normal hematopoiesisOncogenic potentialTET2Important pathogenic mechanismMiR-101Extensive roleMiR-29cHematopoiesisExpressionRegulationPiwi Genes Are Dispensable for Normal Hematopoiesis in Mice
Nolde MJ, Cheng EC, Guo S, Lin H. Piwi Genes Are Dispensable for Normal Hematopoiesis in Mice. PLOS ONE 2013, 8: e71950. PMID: 24058407, PMCID: PMC3751959, DOI: 10.1371/journal.pone.0071950.Peer-Reviewed Original ResearchConceptsPiwi genesHematopoietic stem cellsNormal adult hematopoiesisPIWI protein familyStem cellsStem/progenitor cellsDiverse organismsAdult hematopoiesisProtein familyLong-term hematopoiesisMyeloablative stressCompetitive transplantationTransient expressionHuman leukemia cell linesHSC compartmentLeukemia cell linesGenesProliferative stateNormal hematopoiesisCell typesMIWI2Progenitor cellsLineage reconstitutionHematopoiesisCell proliferation
2010
Compartmentalized organization: a common and required feature of stem cell niches?
Greco V, Guo S. Compartmentalized organization: a common and required feature of stem cell niches? Development 2010, 137: 1586-1594. PMID: 20430743, PMCID: PMC2860245, DOI: 10.1242/dev.041103.Peer-Reviewed Original ResearchConceptsStem cell nicheCell nicheHair follicle stem cell nicheFollicle stem cell nicheAdult stem cell nichesStem cellsStem cell fieldOrgan growthNicheHair regenerationSlow cyclingRecent findingsCell fieldNew growthTissue regenerationRecent studiesCellsGrowthLong-term growthRegenerationProgenyCompartmentsKey questions
2008
MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors
Lu J, Guo S, Ebert BL, Zhang H, Peng X, Bosco J, Pretz J, Schlanger R, Wang JY, Mak RH, Dombkowski DM, Preffer FI, Scadden DT, Golub TR. MicroRNA-Mediated Control of Cell Fate in Megakaryocyte-Erythrocyte Progenitors. Developmental Cell 2008, 14: 843-853. PMID: 18539114, PMCID: PMC2688789, DOI: 10.1016/j.devcel.2008.03.012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CD34Bone Marrow CellsCell DifferentiationCell LineageCells, CulturedErythroid CellsErythropoietinGene Expression RegulationGenes, ReporterHematopoietic Stem CellsHumansIntegrin beta3K562 CellsMegakaryocytesMiceMice, Inbred C57BLMicroRNAsModels, BiologicalPlatelet Membrane Glycoprotein IIbProto-Oncogene Proteins c-mybThrombopoietinConceptsMegakaryocyte-erythrocyte progenitorsLineage specificationTranscription factor MYBMiR-150Cell fateLineage fateRegenerative biologyErythroid cellsFunction experimentsMultipotent cellsMegakaryocytic lineageMiRNA expressionPrimary cellsCritical targetModel systemMicroRNAsProgenitorsFateRegulationCellsImportant participantsMYBLineagesMiRNAsBiology