2023
Serum Response Factor Reduces Gene Expression Noise and Confers Cell State Stability
Zhang J, Wu Q, Hu X, Wang Y, Lu J, Chakraborty R, Martin K, Guo S. Serum Response Factor Reduces Gene Expression Noise and Confers Cell State Stability. Stem Cells 2023, 41: 907-915. PMID: 37386941, PMCID: PMC11009695, DOI: 10.1093/stmcls/sxad051.Peer-Reviewed Original ResearchConceptsMouse pluripotent stem cellsSerum response factorPluripotent stem cellsCell fate stabilityRole of SRFGene expression noiseHeterogeneous gene expressionResponse factorStem cellsNaïve pluripotencyCell state heterogeneityLineage primingExpression noiseActin dynamicsCellular statesPluripotent cellsSRF functionCell statesMechanical signalingGene expressionFunctional modulationCentral mediatorSerum-containing culturesState heterogeneityCellsCell circuits between leukemic cells and mesenchymal stem cells block lymphopoiesis by activating lymphotoxin beta receptor signaling
Feng X, Sun R, Lee M, Chen X, Guo S, Geng H, Müschen M, Choi J, Pereira J. Cell circuits between leukemic cells and mesenchymal stem cells block lymphopoiesis by activating lymphotoxin beta receptor signaling. ELife 2023, 12: e83533. PMID: 36912771, PMCID: PMC10042536, DOI: 10.7554/elife.83533.Peer-Reviewed Original ResearchConceptsMesenchymal stem cellsLymphotoxin beta receptorLeukemic cellsBeta receptorsLeukemic cell growthBone marrow microenvironmentStem cellsTransplant recipientsAML cellsMyeloblastic leukemiaMouse modelBone marrowLeukemia growthLymphotoxin α1β2Marrow microenvironmentPharmacological disruptionLymphopoiesisReceptorsHematopoietic outputMolecular mechanismsErythropoiesisDNA damage response pathwayCell growthCellsPhysiological mechanisms
2022
Integrating mechanical signals into cellular identity
Carley E, King MC, Guo S. Integrating mechanical signals into cellular identity. Trends In Cell Biology 2022, 32: 669-680. PMID: 35337714, PMCID: PMC9288541, DOI: 10.1016/j.tcb.2022.02.006.Peer-Reviewed Original ResearchConceptsDistinct gene expression programsComplex cellular programsGene expression programsLineage-committed cellsPluripotent stem cellsMulticellular organismsExpression programsCellular identityCellular programsMechanical signalsCell typesStem cellsMechanical inputCellsBiochemical inputsFunction correlationGenomeCytoskeletonOrganismsNumber of studiesImportant determinantComplex axisIdentityLarge arrayVivo
2020
YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells
Hartman AA, Scalf SM, Zhang J, Hu X, Chen X, Eastman AE, Yang C, Guo S. YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells. Stem Cell Reports 2020, 14: 730-743. PMID: 32243844, PMCID: PMC7160372, DOI: 10.1016/j.stemcr.2020.03.006.Peer-Reviewed Original ResearchConceptsPluripotency inductionCell typesMouse somatic cellsMultiple stem cell typesHeterologous cell typesStem cell typesPluripotent stem cellsEarly embryogenesisSomatic cellsDistinct functionsMouse cellsMatricellular proteinYAPRecombinant CYR61Stem cellsAutonomous roleCyr61Specific cellsBystander cellsProteinCellsInductionPluripotencyEmbryogenesisControl mechanisms
2019
Collisions on the Busy DNA Highway Set Up Barriers for Reprogramming
Hu X, Guo S. Collisions on the Busy DNA Highway Set Up Barriers for Reprogramming. Cell Stem Cell 2019, 25: 451-453. PMID: 31585090, DOI: 10.1016/j.stem.2019.09.007.Peer-Reviewed Original Research
2018
Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to Pluripotency
Hernandez C, Wang Z, Ramazanov B, Tang Y, Mehta S, Dambrot C, Lee YW, Tessema K, Kumar I, Astudillo M, Neubert TA, Guo S, Ivanova NB. Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to Pluripotency. Cell Stem Cell 2018, 23: 396-411.e8. PMID: 30146411, PMCID: PMC6128737, DOI: 10.1016/j.stem.2018.08.001.Peer-Reviewed Original ResearchConceptsInduced pluripotent stem cellsDNA damage response pathwayAcquisition of pluripotencyDamage response pathwayDNA methylation patternsStem cellsEmbryonic stem cellsESC enhancersPluripotent stem cellsMyc factorsPluripotent stateSomatic genesChromatin decompactionMolecular machineryEpigenetic remodelingEfficient reprogrammingResponse pathwaysSomatic cellsMethylation patternsPluripotencyHuman cellsEpigenomeEnhancerCellsKey role
2013
Piwi Genes Are Dispensable for Normal Hematopoiesis in Mice
Nolde MJ, Cheng EC, Guo S, Lin H. Piwi Genes Are Dispensable for Normal Hematopoiesis in Mice. PLOS ONE 2013, 8: e71950. PMID: 24058407, PMCID: PMC3751959, DOI: 10.1371/journal.pone.0071950.Peer-Reviewed Original ResearchConceptsPiwi genesHematopoietic stem cellsNormal adult hematopoiesisPIWI protein familyStem cellsStem/progenitor cellsDiverse organismsAdult hematopoiesisProtein familyLong-term hematopoiesisMyeloablative stressCompetitive transplantationTransient expressionHuman leukemia cell linesHSC compartmentLeukemia cell linesGenesProliferative stateNormal hematopoiesisCell typesMIWI2Progenitor cellsLineage reconstitutionHematopoiesisCell proliferation
2010
MicroRNA miR-125a controls hematopoietic stem cell number
Guo S, Lu J, Schlanger R, Zhang H, Wang JY, Fox MC, Purton LE, Fleming HH, Cobb B, Merkenschlager M, Golub TR, Scadden DT. MicroRNA miR-125a controls hematopoietic stem cell number. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 14229-14234. PMID: 20616003, PMCID: PMC2922532, DOI: 10.1073/pnas.0913574107.Peer-Reviewed Original ResearchConceptsHematopoietic stem cellsStem cell pool sizeStem cell stateLong-term hematopoietic stem cellsCell-autonomous mannerStem cellsStem cell populationCell pool sizeMiR-125aStem cell numbersHematopoietic stem cell numbersEnzyme DicerImmature hematopoietic progenitorsHematopoietic differentiationMutant animalsCell statesProgenitor cell apoptosisMicroRNA processing enzyme DicerMicroRNA clusterProapoptotic genesHSPC populationsHematopoietic expansionSpecific microRNAsUnique microRNAsHSPC apoptosisCompartmentalized organization: a common and required feature of stem cell niches?
Greco V, Guo S. Compartmentalized organization: a common and required feature of stem cell niches? Development 2010, 137: 1586-1594. PMID: 20430743, PMCID: PMC2860245, DOI: 10.1242/dev.041103.Peer-Reviewed Original ResearchConceptsStem cell nicheCell nicheHair follicle stem cell nicheFollicle stem cell nicheAdult stem cell nichesStem cellsStem cell fieldOrgan growthNicheHair regenerationSlow cyclingRecent findingsCell fieldNew growthTissue regenerationRecent studiesCellsGrowthLong-term growthRegenerationProgenyCompartmentsKey questionsBone progenitor dysfunction induces myelodysplasia and secondary leukaemia
Raaijmakers MH, Mukherjee S, Guo S, Zhang S, Kobayashi T, Schoonmaker JA, Ebert BL, Al-Shahrour F, Hasserjian RP, Scadden EO, Aung Z, Matza M, Merkenschlager M, Lin C, Rommens JM, Scadden DT. Bone progenitor dysfunction induces myelodysplasia and secondary leukaemia. Nature 2010, 464: 852-857. PMID: 20305640, PMCID: PMC3422863, DOI: 10.1038/nature08851.Peer-Reviewed Original ResearchConceptsSpecific mesenchymal cellsMesenchymal cellsHuman bone marrow failureDeletion of Dicer1Osteolineage cellsTissue homeostasisHeterologous cellsDicer1 deletionGene expressionMature osteoblastsRegulatory nicheBone marrow failureDiamond syndromeMesenchymal subsetsStem cellsOsteoprogenitorsReduced expressionDeletionSecondary neoplastic diseaseStromal cellsMarrow failureDICER1CellsHaematopoiesisGenetic abnormalities