2020
Trophoblast-secreted soluble-PD-L1 modulates macrophage polarization and function
Zhang Y, Aldo P, You Y, Ding J, Kaislasuo J, Petersen JF, Lokkegaard E, Peng G, Paidas MJ, Simpson S, Pal L, Guller S, Liu H, Liao AH, Mor G. Trophoblast-secreted soluble-PD-L1 modulates macrophage polarization and function. Journal Of Leukocyte Biology 2020, 108: 983-998. PMID: 32386458, PMCID: PMC8190653, DOI: 10.1002/jlb.1a0420-012rr.Peer-Reviewed Original ResearchConceptsSoluble PD-L1Macrophage polarizationTrophoblast cellsCell death ligand 1PD-L1/PDPD-1 blockageDeath ligand 1Macrophage differentiationModulates macrophage polarizationExpression/secretionDecidual macrophagesHealthy pregnancyPD-L1Pregnant womenTLR4 ligationM2 phenotypeExpression of typeFetal developmentLPS activationMacrophagesIFNPlacenta developmentLigand 1Constitutive productionGestation
2013
Decreased Levels of Folate Receptor‐β and Reduced Numbers of Fetal Macrophages (Hofbauer Cells) in Placentas from Pregnancies with Severe Pre‐Eclampsia
Tang Z, Buhimschi IA, Buhimschi CS, Tadesse S, Norwitz E, Niven‐Fairchild T, Huang S, Guller S. Decreased Levels of Folate Receptor‐β and Reduced Numbers of Fetal Macrophages (Hofbauer Cells) in Placentas from Pregnancies with Severe Pre‐Eclampsia. American Journal Of Reproductive Immunology 2013, 70: 104-115. PMID: 23480364, PMCID: PMC3686834, DOI: 10.1111/aji.12112.Peer-Reviewed Original ResearchConceptsSpontaneous preterm birthPre-eclampsiaAge-matched normotensive controlsPreterm pre-eclampsiaSevere pre eclampsiaPathophysiology of PEPregnancy complicationsPreterm birthNormotensive controlsHofbauer cellsFetal mortalityUnknown etiologyPlacental expressionSPTB groupMacrophage functionPlacentaSevere preFetal macrophagesWestern blottingMajor causeProtein expressionPlacental genesReverse transcription-PCR analysisMarker mRNAsTranscription-PCR analysis
2012
Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells
Tang Z, Niven-Fairchild T, Tadesse S, Norwitz ER, Buhimschi CS, Buhimschi IA, Guller S. Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells. Endocrinology 2012, 154: 471-482. PMID: 23142809, PMCID: PMC3529384, DOI: 10.1210/en.2012-1575.Peer-Reviewed Original ResearchConceptsHofbauer cellsGC treatmentGC administrationCD163 expressionDEX treatmentCD68 mRNASpontaneous preterm birthMacrophage marker genesPlacental explant culturesAntiinflammatory M2 macrophagesHuman term placentaExpression of FRPreterm deliveryPreterm birthCD163 mRNAM2 macrophagesReceptor mRNAGC actionSynthetic GCFetal capillariesTerm placentaPlacental fibroblastsFlow cytometryCD163 proteinFetal macrophages
2009
The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71
Straszewski-Chavez SL, Abrahams VM, Alvero AB, Aldo PB, Ma Y, Guller S, Romero R, Mor G. The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71. Placenta 2009, 30: 939-948. PMID: 19766308, PMCID: PMC2784169, DOI: 10.1016/j.placenta.2009.08.007.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDAntigens, Differentiation, MyelomonocyticApoptosisCell LineChorionic GonadotropinCytokinesFemaleFibronectinsHistocompatibility Antigens Class IHLA AntigensHLA-G AntigensHumansIntercellular Signaling Peptides and ProteinsKeratin-7Leukocyte Common AntigensPregnancyPregnancy Trimester, FirstTelomeraseThy-1 AntigensTrophoblastsVimentinConceptsFirst trimester trophoblast cell lineSwan 71 cellsTrophoblast cell lineFirst trimester trophoblast cellsFibroblast-specific antigenHuman chorionic gonadotrophinPrimary trophoblast cellsTrophoblast cellsHuman telomerase reverse transcriptaseFetal fibronectinCell linesCD90/ThySwan 71Growth factor profileTNF-alpha-induced apoptosisChorionic gonadotrophinCytokeratin 7Specific antigenTelomerase reverse transcriptaseNormal placentaFactor profileKaryotypic abnormalitiesPatient samplesPrimary human cellsAdequate cell numbers