2008
A relaxed active site after exon ligation by the group I intron
Lipchock SV, Strobel SA. A relaxed active site after exon ligation by the group I intron. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 5699-5704. PMID: 18408159, PMCID: PMC2311373, DOI: 10.1073/pnas.0712016105.Peer-Reviewed Original ResearchMeSH KeywordsBinding SitesCrystallography, X-RayExonsHydrogen BondingIntronsKineticsMetalsPolynucleotide LigasesRNARNA SplicingThermodynamicsConceptsActive siteMetal ionsRelaxed active siteActive site metal ionScissile phosphateDirect metal coordinationHydrogen bonding contactsMetal coordinationBonding contactsTransition stateCrystal structureThermodynamic measurementsIonsGround stateStructureCoordinationStructural observationsTertiary interactionsIntron releaseExon ligationGroup I intronPhosphateComplexesReactionSecond step
2000
A Single Adenosine with a Neutral pKa in the Ribosomal Peptidyl Transferase Center
Muth G, Ortoleva-Donnelly L, Strobel S. A Single Adenosine with a Neutral pKa in the Ribosomal Peptidyl Transferase Center. Science 2000, 289: 947-950. PMID: 10937997, DOI: 10.1126/science.289.5481.947.Peer-Reviewed Original Research
1999
A hydrogen-bonding triad stabilizes the chemical transition state of a group I ribozyme
Strobel S, Ortoleva-Donnelly L. A hydrogen-bonding triad stabilizes the chemical transition state of a group I ribozyme. Cell Chemical Biology 1999, 6: 153-165. PMID: 10074469, DOI: 10.1016/s1074-5521(99)89007-3.Peer-Reviewed Original Research
1998
Complementary sets of noncanonical base pairs mediate RNA helix packing in the group I intron active site
Strobel S, Ortoleva-Donnelly L, Ryder S, Cate J, Moncoeur E. Complementary sets of noncanonical base pairs mediate RNA helix packing in the group I intron active site. Nature Structural & Molecular Biology 1998, 5: 60-66. PMID: 9437431, DOI: 10.1038/nsb0198-60.Peer-Reviewed Original Research
1996
Exocyclic Amine of the Conserved G·U Pair at the Cleavage Site of the Tetrahymena Ribozyme Contributes to 5‘-Splice Site Selection and Transition State Stabilization †
Strobel S, Cech T. Exocyclic Amine of the Conserved G·U Pair at the Cleavage Site of the Tetrahymena Ribozyme Contributes to 5‘-Splice Site Selection and Transition State Stabilization †. Biochemistry 1996, 35: 1201-1211. PMID: 8573575, DOI: 10.1021/bi952244f.Peer-Reviewed Original Research
1995
Minor Groove Recognition of the Conserved G⋅U Pair at the Tetrahymena ribozyme Reaction Site
Strobel S, Cech T. Minor Groove Recognition of the Conserved G⋅U Pair at the Tetrahymena ribozyme Reaction Site. Science 1995, 267: 675-679. PMID: 7839142, DOI: 10.1126/science.7839142.Peer-Reviewed Original Research
1993
Tertiary interactions with the internal guide sequence mediate docking of the P1 helix into the catalytic core of the Tetrahymena ribozyme.
Strobel S, Cech T. Tertiary interactions with the internal guide sequence mediate docking of the P1 helix into the catalytic core of the Tetrahymena ribozyme. Biochemistry 1993, 32: 13593-604. PMID: 7504953, DOI: 10.1021/bi00212a027.Peer-Reviewed Original Research
1991
Site-Specific Cleavage of Human Chromosome 4 Mediated by Triple-Helix Formation
Strobel S, Doucette-Stamm L, Riba L, Housman D, Dervan P. Site-Specific Cleavage of Human Chromosome 4 Mediated by Triple-Helix Formation. Science 1991, 254: 1639-1642. PMID: 1836279, DOI: 10.1126/science.1836279.Peer-Reviewed Original ResearchConceptsHuman chromosome 4Human chromosomesChromosome 4Disease genesTriple helix formationEntire candidate regionSpecific DNA segmentsCandidate gene lociSite-specific cleavageGigabase pairsHuman genomeEnzymatic cleavageRecognition motifDNA segmentsGene locusGenomic DNAHuntington's disease geneHuman geneticsTarget siteChromosomesSized fragmentsCandidate regionsCleavageGenomePhysical isolation