2008
A relaxed active site after exon ligation by the group I intron
Lipchock SV, Strobel SA. A relaxed active site after exon ligation by the group I intron. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 5699-5704. PMID: 18408159, PMCID: PMC2311373, DOI: 10.1073/pnas.0712016105.Peer-Reviewed Original ResearchConceptsActive siteMetal ionsRelaxed active siteActive site metal ionScissile phosphateDirect metal coordinationHydrogen bonding contactsMetal coordinationBonding contactsTransition stateCrystal structureThermodynamic measurementsIonsGround stateStructureCoordinationStructural observationsTertiary interactionsIntron releaseExon ligationGroup I intronPhosphateComplexesReactionSecond step
1999
A hydrogen-bonding triad stabilizes the chemical transition state of a group I ribozyme
Strobel S, Ortoleva-Donnelly L. A hydrogen-bonding triad stabilizes the chemical transition state of a group I ribozyme. Cell Chemical Biology 1999, 6: 153-165. PMID: 10074469, DOI: 10.1016/s1074-5521(99)89007-3.Peer-Reviewed Original Research
1998
N2-Methylguanosine is iso-energetic with guanosine in RNA duplexes and GNRA tetraloops
Rife J, Cheng C, Moore P, Strobel S. N2-Methylguanosine is iso-energetic with guanosine in RNA duplexes and GNRA tetraloops. Nucleic Acids Research 1998, 26: 3640-3644. PMID: 9685477, PMCID: PMC147776, DOI: 10.1093/nar/26.16.3640.Peer-Reviewed Original ResearchConceptsM2GGNRA tetraloopRNA duplexesG substitutionSecondary structural stabilityHypermethylated regionsRNA structureNatural RNAPossible functionsRNAWatson-Crick pairsTetraloopNucleotidesN2-methylguanosineRibosomesRRNADuplexMethylationThermodynamic consequencesSubstitutionWide varietyDuplex stabilityPairsFunctionGuanosine
1996
Exocyclic Amine of the Conserved G·U Pair at the Cleavage Site of the Tetrahymena Ribozyme Contributes to 5‘-Splice Site Selection and Transition State Stabilization †
Strobel S, Cech T. Exocyclic Amine of the Conserved G·U Pair at the Cleavage Site of the Tetrahymena Ribozyme Contributes to 5‘-Splice Site Selection and Transition State Stabilization †. Biochemistry 1996, 35: 1201-1211. PMID: 8573575, DOI: 10.1021/bi952244f.Peer-Reviewed Original Research
1994
The 2,6-diaminopurine riboside.5-methylisocytidine wobble base pair: an isoenergetic substitution for the study of G.U pairs in RNA.
Strobel S, Cech T, Usman N, Beigelman L. The 2,6-diaminopurine riboside.5-methylisocytidine wobble base pair: an isoenergetic substitution for the study of G.U pairs in RNA. Biochemistry 1994, 33: 13824-35. PMID: 7524665, DOI: 10.1021/bi00250a037.Peer-Reviewed Original Research
1993
Tertiary interactions with the internal guide sequence mediate docking of the P1 helix into the catalytic core of the Tetrahymena ribozyme.
Strobel S, Cech T. Tertiary interactions with the internal guide sequence mediate docking of the P1 helix into the catalytic core of the Tetrahymena ribozyme. Biochemistry 1993, 32: 13593-604. PMID: 7504953, DOI: 10.1021/bi00212a027.Peer-Reviewed Original Research