The Impact of Second-Shell Nucleotides on Ligand Specificity in Cyclic Dinucleotide Riboswitches
Barth K, Hiller D, Strobel S. The Impact of Second-Shell Nucleotides on Ligand Specificity in Cyclic Dinucleotide Riboswitches. Biochemistry 2024, 63: 487-497. PMID: 38329042, PMCID: PMC11306416, DOI: 10.1021/acs.biochem.3c00586.Peer-Reviewed Original ResearchCyclic di-GMPLigand specificityHigh-throughput mutational analysisLigand contactsRegulate downstream gene expressionDownstream gene expressionDistal nucleotidesTerminal helixSequence differencesChemically similar ligandsRiboswitchNucleotideLigand-binding pocketMutation analysisVariant familiesGene expressionSmall molecule ligandsBinding sitesBinding pocketStructural motifsCrystal structureMolecule ligandsLigandSequenceMotif