2020
Comparative Proteomic Profiling of Unannotated Microproteins and Alternative Proteins in Human Cell Lines
Cao X, Khitun A, Na Z, Dumitrescu DG, Kubica M, Olatunji E, Slavoff SA. Comparative Proteomic Profiling of Unannotated Microproteins and Alternative Proteins in Human Cell Lines. Journal Of Proteome Research 2020, 19: 3418-3426. PMID: 32449352, PMCID: PMC7429271, DOI: 10.1021/acs.jproteome.0c00254.Peer-Reviewed Original ResearchConceptsRibosome profilingHuman cell linesQuantitative mass spectrometry-based proteomicsCell linesMass spectrometry-based proteomicsLabel-free quantitative proteomicsComplex human proteomeLabel-free comparative proteomicsAlternative open reading framesStress-dependent expressionSpectrometry-based proteomicsTissue-specific expressionOpen reading frameComparative proteomic profilingUnannotated ORFsComparative proteomicsUnannotated proteinsMammalian cellsHuman proteomeQuantitative proteomicsDifferential translationReading frameProteomic profilingHuman leukemia cell linesRegulated expression
2013
Chemoproteomic Discovery of Cysteine-Containing Human Short Open Reading Frames
Schwaid AG, Shannon DA, Ma J, Slavoff SA, Levin JZ, Weerapana E, Saghatelian A. Chemoproteomic Discovery of Cysteine-Containing Human Short Open Reading Frames. Journal Of The American Chemical Society 2013, 135: 16750-16753. PMID: 24152191, PMCID: PMC3868496, DOI: 10.1021/ja406606j.Peer-Reviewed Original ResearchConceptsShort open reading framesOpen reading frameReading frameProtein-coding genesProtein-coding potentialGene-finding algorithmsAffinity-based approachRibosome profilingNew genesHuman proteomeMass spectrometry technologyEndogenous RNASpectrometry technologyLabeling approachGenesHuman SEPsPotential importanceInvaluable insightsExperimental approachGenomeProteomeDiscoveryRNABiologyPolypeptide