Expanding the Substrate Tolerance of Biotin Ligase through Exploration of Enzymes from Diverse Species
Slavoff SA, Chen I, Choi YA, Ting AY. Expanding the Substrate Tolerance of Biotin Ligase through Exploration of Enzymes from Diverse Species. Journal Of The American Chemical Society 2008, 130: 1160-1162. PMID: 18171066, PMCID: PMC3501195, DOI: 10.1021/ja076655i.Peer-Reviewed Original ResearchConceptsLigation reactionProtein labeling applicationsDifferential substrate specificitySite-specific conjugationBiotin acceptor domainPhosphine probesStaudinger ligationDiverse speciesBiotin ligaseChemical probesDifferent organismsMass spectrometry assayPyrococcus horikoshiiSubstrate specificityCell biologyUnnatural analoguesLabeling applicationsAcceptor domainsSubstrate toleranceLigasesDifferent speciesSpectrometry assayReactionSpeciesBiotin